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Journal of Cancer Research and Clinical Oncology

Erschienen in:

01.05.2014 | Original Article - Cancer Research

Resveratrol plays dual roles in pancreatic cancer cells

verfasst von: Lei Yang, Liang Yang, Wencong Tian, Jing Li, Jie Liu, Mengmeng Zhu, Yan Zhang, Yinan Yang, Fei Liu, Qiong Zhang, Qianqian Liu, Yanna Shen, Zhi Qi

Erschienen in: Journal of Cancer Research and Clinical Oncology | Ausgabe 5/2014

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Abstract

Purpose

Although the potential anticancer effect of resveratrol (RSV) on pancreatic cancer has been reported, its mechanism was not fully understood. The role of vascular endothelial growth factor B (VEGF-B) in cancer remains controversial. Herein, we aimed to examine whether the anticancer effect of RSV was related to the VEGF-B.

Methods

The effect of RSV on pancreatic cancer cell line (capan-2 cells) was evaluated by CCK-8 assay, Hoechst 33342 staining, and flow cytometry. The mRNA level of VEGF-B was measured by real-time PCR. VEGF-B expression was knockdown by small interfering RNA (siRNA).The protein levels of VEGF-B, glycogen synthase kinase-3 beta (GSK-3β), and Bax were measured by Western blot.

Results

Resveratrol treatment inhibited tumor growth, induced apoptosis, and up-regulated Bax expression in capan-2 cells. The mRNA and protein levels of VEGF-B were up-regulated after RSV treatment. However, VEGF-B siRNA treatment increased the apoptotic rate, and inhibited tumor activator GSK-3β, while Bax expression was not affected. The combination of RSV and VEGF-B siRNA showed significantly higher apoptotic rate in comparison with RSV or VEGF-B siRNA mono-treatment group.

Conclusions

Resveratrol plays dual roles in pancreatic cancer: as a tumor suppressor via the up-regulation of Bax; as a tumor activator via the up-regulation of VEGF-B; and the anticancer effect of RSV is much stronger than the cancer promotion effect. The combination of RSV with pharmacological inhibitor of VEGF-B might, therefore, be a promising modality for clinical pancreatic cancer therapy.

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Titel: Resveratrol plays dual roles in pancreatic cancer cells
verfasst von: Lei Yang
Liang Yang
Wencong Tian
Jing Li
Jie Liu
Mengmeng Zhu
Yan Zhang
Yinan Yang
Fei Liu
Qiong Zhang
Qianqian Liu
Yanna Shen
Zhi Qi
Publikationsdatum: 01.05.2014
Verlag: Springer Berlin Heidelberg
Erschienen in: Journal of Cancer Research and Clinical Oncology / Ausgabe 5/2014
Print ISSN: 0171-5216
Elektronische ISSN: 1432-1335
DOI: https://doi.org/10.1007/s00432-014-1624-4

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Resveratrol plays dual roles in pancreatic cancer cells

Abstract

Purpose

Methods

Results

Conclusions

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Springer Medizin

Abstract

Purpose

Methods

Results

Conclusions

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