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Cytokine secretion profile associated with periportal fibrosis in S. mansoni-infected Egyptian patients

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Abstract

Periportal fibrosis (PPF) is a major pathological consequence of S. mansoni infection. Ultrasonography is a well-established tool for diagnosis and grading of schistosomiasis-related pathology. This work is performed to study the effect of schistosomiasis mansoni infection on the cytokine secretion profile in S. mansoni-infected patients at various grades of fibrosis, as determined by ultrasonography using Cairo Working Group classification. The levels of IL-2, IL-4, IL5, IL-10, IFN-γ, and TNF-α were measured in the absence of in vitro antigen stimulation and after stimulation with worm and egg antigens. Simple intestinal (INT) patients are characterized by strong proliferation to worm antigen and high levels of IL-10 and TNF-α compared to patients at various grades of infection. GradeII (GdII)-infected patients are characterized by higher IL-5 production than are patients with other clinical forms of the disease. Sharp reduction of almost all cytokines in response to both worm and egg antigens was detected in GdIII-infected patients. These results stressed the role of both IL-10 and TNF-α in the early stages of hepatic fibrosis, while IL-5 could be employed as a potential predictive marker for advanced stages. In conclusion, PPF is associated with cytokine production profiles that vary with the magnitude of the fibrosis.

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Acknowledgments

We thank Mr. Yasser Mamdoh for statistical analysis of the results. This research was supported by the Ministry of Health/USAID-funded Schistosomiasis Research Project. The study was done in accordance with the Ministry of Health and Human Service guidelines for Clinical Research and Treatment under a protocol approved by the Schistosomiasis Research Project, VACSERA, Egypt.

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  1. Molecular Biology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), Minofiya University, Sadat City, Egypt

    Roba M. Talaat

  2. Immunology Department, Theodor Bilharz Research Institute, (TBRI), Giza, Egypt

    Azza I. El-Bassiouny

  3. Faculty of Science, Zoology Department, Cairo University, Cairo, Egypt

    Ahmed M. Osman, Ragia Charmy & Maged M. Al-Sherbiny

  4. Tropical Medicine Department, Theodor Bilharz Research Institute, (TBRI), Giza, Egypt

    Magdy Yossif

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  1. Roba M. Talaat
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Correspondence to Roba M. Talaat.

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Talaat, R.M., El-Bassiouny, A.I., Osman, A.M. et al. Cytokine secretion profile associated with periportal fibrosis in S. mansoni-infected Egyptian patients. Parasitol Res 101, 289–299 (2007). https://doi.org/10.1007/s00436-007-0478-y

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