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Ex vivo and in vivo biological behavior of Leishmania (Viannia) shawi

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Abstract

Since the first description of Leishmania (Viannia) shawi, few studies were performed with this parasite. In the present work, the in vivo and ex vivo behavior of L. (Viannia) shawi infection was studied using murine model. Peritoneal macrophages from BALB/c and C57BL/6 mice were infected with promastigotes in the stationary phase of growth; after 24 h, the infection index and nitric oxide (NO) levels in the supernatant of the cultures were analyzed. BALB/c and C57BL/6 mice were infected into the hind footpad, and at each 2 weeks, mice were sacrificed, and the histological changes of the skin inoculation site, parasitism, and humoral immune responses were evaluated during 8 weeks. Ex vivo experiments showed that macrophages of BALB/c presented higher infection index and lesser NO levels than macrophages of C57BL/6. In vivo experiments showed that BALB/c presented higher lesion size than C57BL/6 mice; similarly, the histopathological changes and the parasitism in skin were more exacerbate in BALB/c mice. In draining lymph nodes, the main change was increase of germinative centers, and parasites were detected from 6 weeks pi onwards in both mice strain. IgG was detected in BALB/c mice from 4 weeks, while in C57BL/6, from 6 weeks pi onwards. Taken together, these results indicate that BALB/c showed a classical behavior of susceptibility when compared to C57BL/6 mice.

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Acknowledgements

This work was supported by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) and HCFMUSP-LIM50. It is part of the doctoral thesis of Luiz Felipe D. Passero under a FAPESP fellowship. The authors of this paper would like to thank Profa. Dra. Gabriela Santos-Gomes from Unidade de Leishmanioses, CMDT-LA, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Portugal, for the critical reading of the manuscript.

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Authors and Affiliations

  1. Laboratório de Patologia de Moléstias Infecciosas (LIM-50), Depto. de Patologia, Universidade de São Paulo, São Paulo, SP, Brazil

    Luiz Felipe Domingues Passero, Thaíse Yumie Tomokane, Carlos Eduardo Pereira Corbett & Márcia Dalastra Laurenti

  2. Laboratório de Patologia de Moléstias Infecciosas (LIM-50), Departamento de Patologia, Universidade de São Paulo, Avenida Dr. Arnaldo, 455, 01246-903, São Paulo, Brazil

    Juliano V. Sacomori

  3. Núcleo de Medicina Tropical da Universidade Federal do Pará/Laboratório de Leishmanioses do Instituto Evandro Chagas, Belém, PA, Brazil

    Fernando Tobias da Silveira

  4. Department of Pathology, São Paulo University Medical School, Av. Dr. Arnaldo, 455, 01246-903, São Paulo, SP, Brazil

    Luiz Felipe Domingues Passero

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  1. Luiz Felipe Domingues Passero
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  2. Juliano V. Sacomori
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  3. Thaíse Yumie Tomokane
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  4. Carlos Eduardo Pereira Corbett
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  5. Fernando Tobias da Silveira
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  6. Márcia Dalastra Laurenti
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Correspondence to Luiz Felipe Domingues Passero.

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Passero, L.F.D., Sacomori, J.V., Tomokane, T.Y. et al. Ex vivo and in vivo biological behavior of Leishmania (Viannia) shawi . Parasitol Res 105, 1741–1747 (2009). https://doi.org/10.1007/s00436-009-1614-7

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  • DOI: https://doi.org/10.1007/s00436-009-1614-7

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