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Homolog of allograft inflammatory factor-1 induces macrophage migration during innate immune response in leech

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Abstract

Allograft inflammatory factor-1 (AIF-1) is a 17-kDa cytokine-inducible calcium-binding protein that, in vertebrates, plays an important role in the allograft immune response. Its expression is mostly limited to the monocyte/macrophage lineage. Until recently, AIF-1 was assumed to be a novel molecule involved in inflammatory responses. To clarify this aspect, we have investigated the expression of AIF-1 after bacterial challenge and its potential role in regulating the innate immune response in an invertebrate model, the medicinal leech (Hirudo medicinalis). Analysis of an expressed sequence tag library from the central nervous system of Hirudo revealed the presence of the gene Hmaif-1/alias Hmiba1, showing high homology with vertebrate aif-1. Immunohistochemistry with an anti-HmAIF-1 polyclonal antibody revealed the constitutive presence of this protein in spread CD68+ macrophage-like cells. A few hours after pathogen (bacterial) injection into the body wall, the amount of these immunopositive cells co-expressing HmAIF-1 and the common leucocyte marker CD45 increased at the injected site. Moreover, the recombinant protein HmAIF-1 induced massive angiogenesis and was a potent chemoattractant for macrophages. Following rHmAIF-1 stimulation, macrophage-like cells co-expressed the macrophage marker CD68 and the surface glycoprotein CD45, which, in vertebrates, seems to have a role in the integrin-mediated adhesion of macrophages and in the regulation of the functional responsiveness of cells to chemoattractants. CD45 is therefore probably involved in leech macrophage-like cell activation and migration towards an inflammation site. We have also examined its potential effect on HmAIF-1-induced signalling.

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Acknowledgments

The authors wish to thank the Centro Grandi Attrezzature (CGA) of the University of Insubria and Dr. Laura Pulze for her technical assistance in immunoblot analysis.

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Authors and Affiliations

  1. Department of Biotechnology and Life Sciences, University of Insubria, Via J.H. Dunant 3, 21100, Varese, Italy

    Tilo Schorn, Gianluca Tettamanti, Roberto Valvassori, Magda de Eguileor & Annalisa Grimaldi

  2. Laboratoire PRISM (Protéomique, Réponse Inflammatoire, Spectrométrie de Masse), U 1192 INSERM Université Lille 1, Bât. SN3, Cité Scientifique, 59650, Villeneuve d’Ascq, France

    Francesco Drago & Jacopo Vizioli

Authors
  1. Tilo Schorn
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  2. Francesco Drago
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  3. Gianluca Tettamanti
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  4. Roberto Valvassori
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  5. Magda de Eguileor
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  6. Jacopo Vizioli
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  7. Annalisa Grimaldi
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Corresponding author

Correspondence to Annalisa Grimaldi.

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Tilo Schorn and Francesco Drago contributed equally to this work.

This work was supported by FAR 2011–2012 (Fondi dell’Ateneo per la Ricerca, University of Insubria) to A.G.

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Schorn, T., Drago, F., Tettamanti, G. et al. Homolog of allograft inflammatory factor-1 induces macrophage migration during innate immune response in leech. Cell Tissue Res 359, 853–864 (2015). https://doi.org/10.1007/s00441-014-2058-7

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  • DOI: https://doi.org/10.1007/s00441-014-2058-7

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