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Erschienen in: Journal of Gastroenterology 1/2017

31.03.2016 | Original Article—Alimentary Tract

SCHLAFEN 5 expression correlates with intestinal metaplasia that progresses to gastric cancer

verfasst von: Osmel Companioni Nápoles, Amy C. Tsao, José Miguel Sanz-Anquela, Núria Sala, Catalina Bonet, María Luisa Pardo, Lin Ding, Ornella Simo, Milena Saqui-Salces, Verónica Parra Blanco, Carlos A. Gonzalez, Juanita L. Merchant

Erschienen in: Journal of Gastroenterology | Ausgabe 1/2017

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Abstract

Background

Intestinal metaplasia (IM) is a gastric cancer precursor lesion (GCPL) and an extremely high risk factor for progression to gastric cancer (GC). Clinical guidelines recommend that patients with extensive IM undergo a gastroscopy every 3 years. However, protein biomarkers that indicate a transition from IM to GC are lacking. Our group recently identified an interferon-alpha (IFNα)-responsive gene, Schlafen 4 (Slfn4), in immune cells that correlates with metaplastic changes in Helicobacter-infected mice. We therefore tested the hypothesis that a human homolog of Slfn4, namely, Schlafen 5 (SLFN5), correlates with progression of GCPL to GC.

Methods

Jurkat T-lymphoid and HL-60 myeloid cell lines were treated with IFNα, and SLFN5 mRNA was quantified by quantitative PCR. SLFN5 protein expression in the inflamed gastric mucosa was co-localized to specific immune cell types by immunohistochemistry using CD20, CD2, and MAC2 antibodies. SLFN5 expression was also determined by immunohistochemistry in formalin-fixed paraffin-embedded samples from individuals with non-atrophic gastritis, atrophic gastritis, complete IM, incomplete IM, and GC, respectively.

Results

The IFNα treatment of Jurkat and HL-60 cells induced SLFN5 mRNA. SLFN5 protein was expressed mainly by T lymphocytes in inflamed gastric mucosa. The highest level of SLFN5 expression was observed in patients with IM that progressed to GC. Receiver operating characteristic curves demonstrated that correlating SLFN5 expression with the histologic diagnosis of IM significantly increased the probability of identifying patients who may progress to GC.

Conclusion

In this study population, elevated SLFN5 protein expression in patients with IM correlated with progression to GC.
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Metadaten
Titel
SCHLAFEN 5 expression correlates with intestinal metaplasia that progresses to gastric cancer
verfasst von
Osmel Companioni Nápoles
Amy C. Tsao
José Miguel Sanz-Anquela
Núria Sala
Catalina Bonet
María Luisa Pardo
Lin Ding
Ornella Simo
Milena Saqui-Salces
Verónica Parra Blanco
Carlos A. Gonzalez
Juanita L. Merchant
Publikationsdatum
31.03.2016
Verlag
Springer Japan
Erschienen in
Journal of Gastroenterology / Ausgabe 1/2017
Print ISSN: 0944-1174
Elektronische ISSN: 1435-5922
DOI
https://doi.org/10.1007/s00535-016-1202-4

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