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01.01.2016 | Original Article

Osteoclast activators are elevated in intervertebral disks with Modic changes among patients operated for herniated nucleus pulposus

verfasst von: Matias Torkki, Marja-Leena Majuri, Henrik Wolff, Tatu Koskelainen, Marianne Haapea, Jaakko Niinimäki, Harri Alenius, Jeffrey Lotz, Jaro Karppinen

Erschienen in: European Spine Journal | Ausgabe 1/2016

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Abstract

Purpose

Modic changes (MC) are associated with low back pain (LBP). Inflammation is considered as a key factor that triggers symptoms in especially type I MC, but so far of the potential inflammatory candidates only TNFα has been linked to MC. The objective of the study was to analyze a set of inflammatory mediators in human surgical disk samples and quantify their association with MC in the adjacent vertebral bodies.

Methods

The study sample consisted of 51 intervertebral disk tissue specimens; 20 ‘No MC’ disks, 19 ‘Type I MC’ disks, and 12 ‘Type II MC’ disks. mRNA expression of 46 cytokines was quantified from isolated RNA. Tissue samples were stained using hematoxylin and eosin, toluidine blue, Herovici, CD68 and CD163.

Results

No significant differences were found in the amount of macrophages or presence of chondrocyte conglomerates between the MC groups. Of the multiple genes tested, statistically significant associations were observed for M-CSF1 (p = 0.028), RANKL (p = 0.035), RUNX1 (p = 0.032), and RUNX2 (p = 0.047) that were increased in ‘Type II MC,’ while OSCAR (p = 0.042) was increased in ‘Type I MC’ group compared to ‘No MC.’

Conclusions

Since these cytokines are related to differentiation and proliferation of osteoclasts, our data suggest that the stimulation of vertebral osteoclasts by factors secreted by disk tissue is involved in the pathophysiology of MC.

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Titel: Osteoclast activators are elevated in intervertebral disks with Modic changes among patients operated for herniated nucleus pulposus
verfasst von: Matias Torkki
Marja-Leena Majuri
Henrik Wolff
Tatu Koskelainen
Marianne Haapea
Jaakko Niinimäki
Harri Alenius
Jeffrey Lotz
Jaro Karppinen
Publikationsdatum: 01.01.2016
Verlag: Springer Berlin Heidelberg
Erschienen in: European Spine Journal / Ausgabe 1/2016
Print ISSN: 0940-6719
Elektronische ISSN: 1432-0932
DOI: https://doi.org/10.1007/s00586-015-3897-y

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Osteoclast activators are elevated in intervertebral disks with Modic changes among patients operated for herniated nucleus pulposus

Abstract

Purpose

Methods

Results

Conclusions

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