Erschienen in:
01.02.2013 | Original Article
Nuclear factor κB activity correlates with the progression and prognosis of pancreatic cancer in a mouse model
verfasst von:
Kenei Furukawa, Tadashi Uwagawa, Koichiro Haruki, Yuki Fujiwara, Tomonori Iida, Hiroaki Shiba, Takeyuki Misawa, Toya Ohashi, Katsuhiko Yanaga
Erschienen in:
Surgery Today
|
Ausgabe 2/2013
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Abstract
Introduction
Constitutive NF-κB activation is considered to play a key role in the
aggressive behavior of pancreatic cancer. Although NF-κB in tumors may contribute to
aggressive characteristic features via transcription of angiogenesis and invasion-related
factors, there is no definitive evidence showing a correlation between quantitated NF-κB
activity and prognosis. In this study, we quantitated NF-κB activity of various human
pancreatic cancer cell lines and evaluated whether NF-κB activity was related to tumor
progression and prognosis for pancreatic cancer in mice.
Materials and methods
We quantitated NF-κB activity in six pancreatic cancer cell lines
(AsPC-1, BxPC-3, Capan-2, MIAPaCa-2, Panc-1 and PL45) and evaluated downstream
target genes of NF-κB such as VEGF, IL-8 and MMP-9 in vitro. Next, we evaluated tumor
progression and prognosis using subcutaneous tumor model in vivo between cell lines with
the highest and lowest NF-κB activity.
Results
BxPC-3 had the highest and AsPC-1 had the lowest NF-κB activity in the 6 cell
lines. Expression of VEGF, IL-8 and MMP-9 in BxPC-3 was significantly higher than those
in AsPC-1 cells in vitro (p < 0.001) and tumor growth in BxPC-3 was faster than that in
AsPC-1 group (p < 0.001) resulting in worse survival in vivo (p = 0.0339).
Conclusion
These results suggested that NF-κB activity is related to expression of its
downstream target genes, tumor progression and prognosis in experimental pancreatic
cancer model.