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Acta Neurochirurgica

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01.05.2013 | Experimental research - Spine

Enhanced neuroregenerative effects by scaffold for the treatment of a rat spinal cord injury with Wnt3a-secreting fibroblasts

verfasst von: Jin Hoon Park, Joongkee Min, Se Rim Baek, Seong Who Kim, Il Keun Kwon, Sang Ryong Jeon

Erschienen in: Acta Neurochirurgica | Ausgabe 5/2013

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Abstract

Background

Wnt proteins are bifunctional axon guidance molecules, several of which appear to mediate guidance of corticospinal tract axons along the spinal cord. Here, we studied increasing effect on regeneration by Wnt-containing alginate scaffolds on spinal cord injury (SCI).

Methods

A total of 32 rats were injured at the T7–8 level with an NYU impactor. According to transplantation materials, rats were classified into four groups: a Wnt3a-secreting fibroblast transplantation group (Wnt group, n = 8), a Wnt3a-secreting fibroblast with alginate transplantation group (Wnt + alginate group, n = 8), an alginate transplantation group (alginate group, n = 8), and a contusion-only group (sham group, n = 8). Behavioral tests were performed on the first, second, and third days after injury, and then weekly for 8 weeks. Five of the eight rats from each group were selected for manganese-enhanced magnetic resonance imaging (ME-MRI). Two rats from each group were examined for GAP43 and MAP2 expression using monoclonal and polyclonal primary antibodies, respectively.

Results

Seven weeks after transplantation (8 weeks after SCI), Wnt + alginate group rats achieved an average Basso–Beattie–Bresnahan locomotor score of 19.0, which was significantly higher than that of other groups. ME-MRI at 8 weeks after SCI revealed significantly higher relative signal intensities in the Wnt + alginate group. Gap43 and Map2 immunostaining, showed strong positive in the Wnt + alginate group.

Conclusion

The Wnt + alginate complex exerted significantly enhanced recovery in a rat SCI model compared to alginate or Wnt3a alone. These results suggest that alginate scaffolds facilitate the regeneration of axon working with Wnt3a protein that promotes regeneration of the injured spinal cord.

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Titel: Enhanced neuroregenerative effects by scaffold for the treatment of a rat spinal cord injury with Wnt3a-secreting fibroblasts
verfasst von: Jin Hoon Park
Joongkee Min
Se Rim Baek
Seong Who Kim
Il Keun Kwon
Sang Ryong Jeon
Publikationsdatum: 01.05.2013
Verlag: Springer Vienna
Erschienen in: Acta Neurochirurgica / Ausgabe 5/2013
Print ISSN: 0001-6268
Elektronische ISSN: 0942-0940
DOI: https://doi.org/10.1007/s00701-013-1663-7

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Enhanced neuroregenerative effects by scaffold for the treatment of a rat spinal cord injury with Wnt3a-secreting fibroblasts

Abstract

Background

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Results

Conclusion

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Abstract

Background

Methods

Results

Conclusion

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