Erschienen in:
01.06.2013 | Translational Neurosciences - Original Article
Do MAO A and MAO B utilize the same mechanism for the C–H bond cleavage step in catalysis? Evidence suggesting differing mechanisms
verfasst von:
R. Orru, M. Aldeco, D. E. Edmondson
Erschienen in:
Journal of Neural Transmission
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Ausgabe 6/2013
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Abstract
The detailed molecular mechanism proposed for the MAO-catalyzed oxidation of amines has been controversial with the basic assumption that both MAO A and MAO B follow the same pathway for the C–H bond cleavage step. Using the mechanistic approach of investigation of electronic effects of various benzylamine ring substituents in experiments at pH 9.0, human MAO A exhibits a kinetic behavior characteristic of an H+ abstraction, while human MAO B exhibits kinetic properties characteristic of a H− abstraction. These results lead to the conclusion that the assumption that MAO A and MAO B follow identical mechanisms is incorrect.