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Archives of Virology

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01.07.2008 | Brief Report

Construction of an infectious full-length cDNA clone of potato virus M

verfasst von: S. Flatken, V. Ungewickell, W. Menzel, E. Maiss

Erschienen in: Archives of Virology | Ausgabe 7/2008

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Abstract

An infectious full-length cDNA clone of potato virus M (PVM) was produced. Total RNA was extracted from PVM-infected Nicotiana hesperis plants and used for cDNA synthesis. Subsequent RT-PCR produced two DNA fragments of about 5.5 and 3.2 kbp, which were ligated downstream of an enhanced 35S cauliflower mosaic virus promoter. After cloning of the enhanced 35S promoter with the PVM sequence into a modified pBIN19 plasmid and electroporation of Agrobacterium tumefaciens, the agroinoculated PVM full-length clone (pPVM-flc) led to systemic PVM infections in different host plants, causing symptoms indistinguishable from those caused by wild-type PVM.

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Titel: Construction of an infectious full-length cDNA clone of potato virus M
verfasst von: S. Flatken
V. Ungewickell
W. Menzel
E. Maiss
Publikationsdatum: 01.07.2008
Verlag: Springer Vienna
Erschienen in: Archives of Virology / Ausgabe 7/2008
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-008-0127-5

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Construction of an infectious full-length cDNA clone of potato virus M

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