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Archives of Virology

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01.09.2010 | Brief Report

Identification of the 3C-protease-mediated 2A/2B and 2B/2C cleavage sites in the nonstructural polyprotein precursor of a dicistrovirus lacking the NPGP motif

verfasst von: Nobuhiko Nakashima, Jun Ishibashi

Erschienen in: Archives of Virology | Ausgabe 9/2010

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Abstract

Dicistroviruses have motifs for picornavirus 2C, 3C, and 3D proteins in their nonstructural polyprotein C-terminal region. The proteins from the nonstructural, N-terminal region of the polyprotein remain to be characterized. We have identified 3C-mediated cleavage sites in the N-terminal region of the nonstructural polyprotein of the dicistrovirus Plautia stali intestine virus (PSIV). The 2B/2C cleavage site mapped to amino acids (aa) 408–409 (QD). 2B/2C cleavage sites were suggested to be conserved in dicistroviruses. The most N-terminal PSIV cleavage site was aa 286–287 (QS). Including previous results, the polyprotein contains nine proteins arranged as follows: 2A, 2B, 2C, 3A, 3B1, 3B2, 3B3, 3C, and 3D.

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Titel: Identification of the 3C-protease-mediated 2A/2B and 2B/2C cleavage sites in the nonstructural polyprotein precursor of a dicistrovirus lacking the NPGP motif
verfasst von: Nobuhiko Nakashima
Jun Ishibashi
Publikationsdatum: 01.09.2010
Verlag: Springer Vienna
Erschienen in: Archives of Virology / Ausgabe 9/2010
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-010-0723-z

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Identification of the 3C-protease-mediated 2A/2B and 2B/2C cleavage sites in the nonstructural polyprotein precursor of a dicistrovirus lacking the NPGP motif

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