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Erschienen in: Archives of Virology 10/2010

01.10.2010 | Brief Report

Rapid detection of tobacco mosaic virus using the reverse transcription loop-mediated isothermal amplification method

verfasst von: Yanhua Liu, Zhide Wang, Yumei Qian, Jianmin Mu, Lili Shen, Fenglong Wang, Jinguang Yang

Erschienen in: Archives of Virology | Ausgabe 10/2010

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Abstract

Tobacco mosaic virus (TMV) can cause a severe disease that is capable of greatly reducing tobacco quality and yield. In this study, a one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for detection of TMV. The concentration of Mg2+, reaction temperature and reaction time of the RT-LAMP were optimized to 5 mM, 65°C, and 60 min, respectively. The detection limit of the method was 100 times higher than that of RT-PCR. Visual inspection of RT-LAMP amplification demonstrated that positive and negative reactions exhibit distinctly different colours in daylight. Our results demonstrate that the method is stable, sensitive and specific.
Literatur
1.
Zurück zum Zitat Zhu XC, Wang YT, Wang ZF (2002) Tobacco diseases of China. China Agricultural Press, Beijing Zhu XC, Wang YT, Wang ZF (2002) Tobacco diseases of China. China Agricultural Press, Beijing
2.
Zurück zum Zitat Wang JH, Wang YQ, Ji XC (2005) Advances on techniques for detection of plant viruses. Chin J Trop Agric 25(3):71–75 Wang JH, Wang YQ, Ji XC (2005) Advances on techniques for detection of plant viruses. Chin J Trop Agric 25(3):71–75
3.
Zurück zum Zitat Sun ZF, Hu CQ, Ren CH, Shen Q (2006) Sensitive and rapid detection of infectious hypodermal and hematopoietic necrosis virus (IHHNV) in shrimps by loop-mediated isothermal amplification. J Virol Methods 131(1):41–46CrossRefPubMed Sun ZF, Hu CQ, Ren CH, Shen Q (2006) Sensitive and rapid detection of infectious hypodermal and hematopoietic necrosis virus (IHHNV) in shrimps by loop-mediated isothermal amplification. J Virol Methods 131(1):41–46CrossRefPubMed
4.
Zurück zum Zitat Notomi T, Okayama H, Masubuchi H, Yonekawa T, Watanabe K, Amino N, Hase T (2000) Loop-mediated isothermal amplification of DNA. Nucleic Acids Res 28(12):e63CrossRefPubMed Notomi T, Okayama H, Masubuchi H, Yonekawa T, Watanabe K, Amino N, Hase T (2000) Loop-mediated isothermal amplification of DNA. Nucleic Acids Res 28(12):e63CrossRefPubMed
5.
Zurück zum Zitat Nagamine K, Hase T, Notomi T (2002) Accelerated reaction by loop-mediated isothermal amplification using loop primers. Mol Cell Probes 16:223–229CrossRefPubMed Nagamine K, Hase T, Notomi T (2002) Accelerated reaction by loop-mediated isothermal amplification using loop primers. Mol Cell Probes 16:223–229CrossRefPubMed
6.
Zurück zum Zitat Fukuta S, Iida T, Mizukami Y, Ishida A, Ueda J, Kanbe M (2003) Detection of Japanese yam mosaic virus by RT-LAMP. Arch Virol 148:1713–1720CrossRefPubMed Fukuta S, Iida T, Mizukami Y, Ishida A, Ueda J, Kanbe M (2003) Detection of Japanese yam mosaic virus by RT-LAMP. Arch Virol 148:1713–1720CrossRefPubMed
7.
Zurück zum Zitat Wang Y, Lan QK, Zhao X (2009) Development and application of loop-mediated isothermal amplification for detection of genetically modified crops. Sci Agric Sin 42(4):1473–1477 Wang Y, Lan QK, Zhao X (2009) Development and application of loop-mediated isothermal amplification for detection of genetically modified crops. Sci Agric Sin 42(4):1473–1477
8.
Zurück zum Zitat Yang JG, Zhang S, Shen LL, Qian YM, Chen DX, Wang CS, Huang J, Wang FL (2010) Establish and application of multiple RT-PCR for detection of TMV, CMV and PVY in tobacco. Acta Tabacaria Sin 16(4):80–84 Yang JG, Zhang S, Shen LL, Qian YM, Chen DX, Wang CS, Huang J, Wang FL (2010) Establish and application of multiple RT-PCR for detection of TMV, CMV and PVY in tobacco. Acta Tabacaria Sin 16(4):80–84
9.
Zurück zum Zitat Blomström AL, Hakhverdyan M, Reid SM, Dukes JP, King DP, Belák S, Berg M (2008) A one-step reverse transcriptase loop-mediated isothermal amplification assay for simple and rapid detection of swine vesicular disease virus. J Virol Methods 147(1):188–193CrossRefPubMed Blomström AL, Hakhverdyan M, Reid SM, Dukes JP, King DP, Belák S, Berg M (2008) A one-step reverse transcriptase loop-mediated isothermal amplification assay for simple and rapid detection of swine vesicular disease virus. J Virol Methods 147(1):188–193CrossRefPubMed
10.
Zurück zum Zitat Soliman H, El-Matbouli M (2006) Reverse transcription loop-mediated isothermal amplification (RT-LAMP) for rapid detection of viral hemorrhagic septicaemia virus (VHS). Vet Microbiol 114(3–4):205–213CrossRefPubMed Soliman H, El-Matbouli M (2006) Reverse transcription loop-mediated isothermal amplification (RT-LAMP) for rapid detection of viral hemorrhagic septicaemia virus (VHS). Vet Microbiol 114(3–4):205–213CrossRefPubMed
11.
Zurück zum Zitat Li Q, Cy Xue, Qin JP, Zhou QF, Chen F, Bi YZ, Cao YC (2009) An improved reverse transcription loop-mediated isothermal amplification assay for sensitive and specific detection of Newcastle disease virus. Arch Virol 154:1433–1440CrossRefPubMed Li Q, Cy Xue, Qin JP, Zhou QF, Chen F, Bi YZ, Cao YC (2009) An improved reverse transcription loop-mediated isothermal amplification assay for sensitive and specific detection of Newcastle disease virus. Arch Virol 154:1433–1440CrossRefPubMed
12.
Zurück zum Zitat Gunimaladevi I, Kono T, Lapatra SE, Sakai M (2005) A loop mediated isothermal amplification (LAMP) method for detection of infectious hematopoietic necrosis virus (IHNV) in rainbow trout (Oncorhynchus mykiss). Arch Virol 150(5):899–909CrossRefPubMed Gunimaladevi I, Kono T, Lapatra SE, Sakai M (2005) A loop mediated isothermal amplification (LAMP) method for detection of infectious hematopoietic necrosis virus (IHNV) in rainbow trout (Oncorhynchus mykiss). Arch Virol 150(5):899–909CrossRefPubMed
13.
Zurück zum Zitat Kono T, Savan R, Sakai M, Itami T (2004) Detection of white spot syndrome virus in shrimp by loop-mediated isothermal amplification. J Virol Methods 115:59–65CrossRefPubMed Kono T, Savan R, Sakai M, Itami T (2004) Detection of white spot syndrome virus in shrimp by loop-mediated isothermal amplification. J Virol Methods 115:59–65CrossRefPubMed
14.
Zurück zum Zitat Teng PH, Chen CL, Sung PF, Lee FC, Ou BR, Lee PY (2007) Specific detection of reverse transcription-loop-mediated isothermal amplification amplicons for Taura syndrome virus by colorimetric dot-blot hybridization. J Virol Methods 146(1–2):317–326CrossRefPubMed Teng PH, Chen CL, Sung PF, Lee FC, Ou BR, Lee PY (2007) Specific detection of reverse transcription-loop-mediated isothermal amplification amplicons for Taura syndrome virus by colorimetric dot-blot hybridization. J Virol Methods 146(1–2):317–326CrossRefPubMed
15.
Zurück zum Zitat Mori Y, Nagamine K, Tomita N, Notomi T (2001) Detection of loop-mediated isothermal amplification reaction by turbidity derived from magnesium pyrophosphate formation. Biochem Biophys Res Commun 289:150–154CrossRefPubMed Mori Y, Nagamine K, Tomita N, Notomi T (2001) Detection of loop-mediated isothermal amplification reaction by turbidity derived from magnesium pyrophosphate formation. Biochem Biophys Res Commun 289:150–154CrossRefPubMed
Metadaten
Titel
Rapid detection of tobacco mosaic virus using the reverse transcription loop-mediated isothermal amplification method
verfasst von
Yanhua Liu
Zhide Wang
Yumei Qian
Jianmin Mu
Lili Shen
Fenglong Wang
Jinguang Yang
Publikationsdatum
01.10.2010
Verlag
Springer Vienna
Erschienen in
Archives of Virology / Ausgabe 10/2010
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-010-0746-5

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