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Archives of Virology

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01.02.2013 | Brief Report

Development of a full-length infectious clone of sunflower chlorotic mottle virus (SuCMoV)

verfasst von: N. Bejerman, F. Giolitti, S. de Breuil, S. Lenardon

Erschienen in: Archives of Virology | Ausgabe 2/2013

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Abstract

A full-length cDNA clone (p35SuCMoV) of the sunflower chlorotic mottle virus common strain (SuCMoV-C) genomic RNA was constructed. Three cDNA fragments covering the whole genome of SuCMoV-C were cloned between a cauliflower mosaic virus 35S promoter and a nopaline synthase terminator. Mechanical inoculation of sunflower and Nicotiana occidentalis seedlings with p35SuCMoV DNA led to systemic infection. Symptoms induced by p35SuCMoV were similar to those caused by the wild-type SuCMoV-C but appeared four days later. Infection was confirmed by a western blot test, electron microscopy, RT-PCR and inoculation of progeny virions to sunflower seedlings. This is the first report about the construction of a biologically active, full-length cDNA copy of the SuCMoV-C RNA genome.

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Titel: Development of a full-length infectious clone of sunflower chlorotic mottle virus (SuCMoV)
verfasst von: N. Bejerman
F. Giolitti
S. de Breuil
S. Lenardon
Publikationsdatum: 01.02.2013
Verlag: Springer Vienna
Erschienen in: Archives of Virology / Ausgabe 2/2013
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-012-1497-2

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