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Archives of Virology

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01.12.2013 | Original Article

Isolation and characterization of two H5N1 influenza viruses from swine in Jiangsu Province of China

verfasst von: Liang He, Guo Zhao, Lei Zhong, Qingtao Liu, Zhiqiang Duan, Min Gu, Xiaoquan Wang, Xiaowen Liu, Xiufan Liu

Erschienen in: Archives of Virology | Ausgabe 12/2013

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Abstract

Pigs are susceptible to infection with both human and avian influenza A viruses and are considered intermediate hosts that facilitate virus reassortment. Although H5N1 virus has spread to a wide range of avian and mammalian species, data about swine H5N1 isolates are scarce. To determine whether Asian H5N1 influenza viruses had been transmitted to pigs, a total of 1,107 nasal swab samples from healthy swine were collected from 2008 to 2009 in Jiangsu province of eastern China. In this survey, two H5N1 viruses A/swine/Jiangsu/1/2008 (JS/08) and A/swine/Jiangsu/2/2009 (JS/09) were isolated and identified. Phylogenetic analysis showed that JS/08 and JS/09 belonged to clade 7 and clade 2.3.4, respectively, and shared over 99.0 % sequence identity with poultry H5N1 isolates of the same clade in China. Receptor specificity analysis also showed that both of the swine H5N1 isolates bound preferentially to avian-type receptors. However, experiments in mammals indicated that JS/09 was moderately pathogenic to mice without prior adaption, whereas JS/08 had limited ability to replicate. Our findings suggest that pigs are naturally infected with avian H5N1 virus and highlight the potential threat to public health due to adaption or reassortment of H5N1 virus in this species.

Auewarakul P, Suptawiwat O, Kongchanagul A, Sangma C, Suzuki Y, Ungchusak K, Louisirirotchanakul S, Lerdsamran H, Pooruk P, Thitithanyanont A, Pittayawonganon C, Guo CT, Hiramatsu H, Jampangern W, Chunsutthiwat S, Puthavathana P (2007) An avian influenza H5N1 virus that binds to a human-type receptor. J Virol 81:9950–9955PubMedCrossRef

Bi YH, Fu GH, Chen J, Peng JS, Sun YP, Wang JJ, Pu JA, Zhang Y, Gao HJ, Ma GP, Tian FL, Brown IH, Liu JH (2010) Novel swine influenza virus reassortants in pigs, China. Emerg Inf Dis 16:1162–1164CrossRef

Castrucci MR, Donatelli I, Sidoli L, Barigazzi G, Kawaoka Y, Webster RG (1993) Genetic reassortment between avian and human influenza A viruses in Italian pigs. Virology 193:503–506PubMedCrossRef

Chen LM, Davis CT, Zhou H, Cox NJ, Donis RO (2008) Genetic compatibility and virulence of reassortants derived from contemporary avian H5N1 and human H3N2 influenza A viruses. PloS Path 4:e1000072CrossRef

Choi YK, Nguyen TD, Ozaki H, Webby RJ, Puthavathana P, Buranathal C, Chaisingh A, Auewarakul P, Hanh NTH, Ma SK, Hui PY, Guan Y, Peiris JSM, Webster RG (2005) Studies of H5N1 influenza virus infection of pigs by using viruses isolated in Vietnam and Thailand in 2004. J Virol 79:10821–10825PubMedCrossRef

Claas EC, Osterhaus AD, van Beek R, De Jong JC, Rimmelzwaan GF, Senne DA, Krauss S, Shortridge KF, Webster RG (1998) Human influenza A H5N1 virus related to a highly pathogenic avian influenza virus. Lancet 351:472–477PubMedCrossRef

Cong YL, Wang GM, Guan ZH, Chang SA, Zhang QP, Yang GL, Wang WL, Meng QF, Ren WM, Wang CF, Ding ZA (2010) Reassortant between human-like H3N2 and avian H5 subtype influenza A viruses in pigs: a potential public health risk. PloS One 5:e12591PubMedCrossRef

Garten RJ, Davis CT, Russell CA, Shu B, Lindstrom S, Balish A, Sessions WM, Xu X, Skepner E, Deyde V, Okomo-Adhiambo M, Gubareva L, Barnes J, Smith CB, Emery SL, Hillman MJ, Rivailler P, Smagala J, de Graaf M, Burke DF, Fouchier RA, Pappas C, Alpuche-Aranda CM, Lopez-Gatell H, Olivera H, Lopez I, Myers CA, Faix D, Blair PJ, Yu C, Keene KM, Dotson PD Jr, Boxrud D, Sambol AR, Abid SH, St George K, Bannerman T, Moore AL, Stringer DJ, Blevins P, Demmler-Harrison GJ, Ginsberg M, Kriner P, Waterman S, Smole S, Guevara HF, Belongia EA, Clark PA, Beatrice ST, Donis R, Katz J, Finelli L, Bridges CB, Shaw M, Jernigan DB, Uyeki TM, Smith DJ, Klimov AI, Cox NJ (2009) Antigenic and genetic characteristics of swine-origin 2009 A(H1N1) influenza viruses circulating in humans. Science 325:197–201PubMedCrossRef

Hatta M, Gao P, Halfmann P, Kawaoka Y (2001) Molecular basis for high virulence of Hong Kong H5N1 influenza A viruses. Science 293:1840–1842PubMedCrossRef

10.

Hoffmann E, Stech J, Guan Y, Webster RG, Perez DR (2001) Universal primer set for the full-length amplification of all influenza A viruses. Arch Virol 146:2275–2289PubMedCrossRef

11.

Imai M, Watanabe T, Hatta M, Das SC, Ozawa M, Shinya K, Zhong GX, Hanson A, Katsura H, Watanabe S, Li CJ, Kawakami E, Yamada S, Kiso M, Suzuki Y, Maher EA, Neumann G, Kawaoka Y (2012) Experimental adaptation of an influenza H5 HA confers respiratory droplet transmission to a reassortant H5 HA/H1N1 virus in ferrets. Nature 486:420–428PubMed

12.

Ito T, Couceiro JN, Kelm S, Baum LG, Krauss S, Castrucci MR, Donatelli I, Kida H, Paulson JC, Webster RG, Kawaoka Y (1998) Molecular basis for the generation in pigs of influenza A viruses with pandemic potential. J Virol 72:7367–7373PubMed

13.

Jiang WM, Liu S, Chen J, Hou GY, Li JP, Cao YF, Zhuang QY, Li Y, Huang BX, Chen JM (2010) Molecular epidemiological surveys of H5 subtype highly pathogenic avian influenza viruses in poultry in China during 2007–2009. J Gen Virol 91:2491–2496PubMedCrossRef

14.

Kida H, Ito T, Yasuda J, Shimizu Y, Itakura C, Shortridge KF, Kawaoka Y, Webster RG (1994) Potential for transmission of avian influenza viruses to pigs. J Gen Virol 75:2183–2188PubMedCrossRef

15.

Lee MS, Chang PC, Shien JH, Cheng MC, Shieh HK (2001) Identification and subtyping of avian influenza viruses by reverse transcription-PCR. J Virol Methods 97:13–22PubMedCrossRef

16.

Li CJ, Hatta M, Nidom CA, Muramoto Y, Watanabe S, Neumann G, Kawaoka Y (2010) Reassortment between avian H5N1 and human H3N2 influenza viruses creates hybrid viruses with substantial virulence. P Natl Acad Sci USA 107:4687–4692CrossRef

17.

Li Y, Zhang X, Xu Q, Fu Q, Zhu Y, Chen S, Peng D, Liu X (2013) Characterisation and haemagglutinin gene epitope mapping of a variant strain of H5N1 subtype avian influenza virus. Vet Microbiol 162:614–622PubMedCrossRef

18.

Li YB, Shi JZ, Zhong GX, Deng GH, Tian GB, Ge JY, Zeng XY, Song JS, Zhao DM, Liu LL, Jiang YP, Guan YT, Bu ZG, Chen HL (2010) Continued evolution of H5N1 influenza viruses in wild birds, domestic poultry, and humans in China from 2004 to 2009. J Virol 84:8389–8397PubMedCrossRef

19.

Li Z, Chen H, Jiao P, Deng G, Tian G, Li Y, Hoffmann E, Webster RG, Matsuoka Y, Yu K (2005) Molecular basis of replication of duck H5N1 influenza viruses in a mammalian mouse model. J Virol 79:12058–12064PubMedCrossRef

20.

Lipatov AS, Kwon YK, Sarmento LV, Lager KM, Spackman E, Suarez DL, Swayne DE (2008) Domestic pigs have low susceptibility to H5N1 highly pathogenic avian influenza viruses. PloS Path 4:e1000102CrossRef

21.

Liu QF, Ma JJ, Kou Z, Pu J, Lei FM, Li TX, Liu JH (2010) Characterization of a highly pathogenic avian influenza H5N1 clade 2.3.4 virus isolated from a tree sparrow. Virus Res 147:25–29PubMedCrossRef

22.

Liu W, Wei MT, Tong YG, Tang F, Zhang L, Fang LQ, Yang H, Cao WC (2011) Seroprevalence and genetic characteristics of five subtypes of influenza A viruses in the Chinese pig population: a pooled data analysis. Vet J 187:200–206PubMedCrossRef

23.

Maines TR, Chen LM, Matsuoka Y, Chen HL, Rowe T, Ortin J, Falcon A, Hien NT, Mai LQ, Sedyaningsih ER, Harun S, Tumpey TM, Donis RO, Cox NJ, Subbarao K, Katz JM (2006) Lack of transmission of H5N1 avian-human reassortant influenza viruses in a ferret model. P Natl Acad Sci USA 103:12121–12126CrossRef

24.

Negovetich NJ, Webster RG (2010) Thermostability of subpopulations of H2N3 influenza virus isolates from mallard ducks. J Virol 84:9369–9376PubMedCrossRef

25.

Nidom CA, Takano R, Yamada S, Sakai-Tagawa Y, Daulay S, Aswadi D, Suzuki T, Suzuki Y, Shinya K, Iwatsuki-Horimoto K, Muramoto Y, Kawaoka Y (2010) Influenza A (H5N1) viruses from pigs, Indonesia. Emerg Inf Dis 16:1515–1523CrossRef

26.

Octaviani CP, Ozawa M, Yamada S, Goto H, Kawaoka Y (2010) High level of genetic compatibility between swine-origin H1N1 and highly pathogenic avian H5N1 influenza viruses. J Virol 84:10918–10922PubMedCrossRef

27.

Peiris JSM, de Jong MD, Guan Y (2007) Avian influenza virus (H5N1): a threat to human health. Clin Microbiol Rev 20:243–267PubMedCrossRef

28.

Qiu BF, Liu WJ, Peng DX, Hu SL, Tang YH, Liu XF (2009) A reverse transcription-PCR for subtyping of the neuraminidase of avian influenza viruses. J Virol Methods 155:193–198PubMedCrossRef

29.

Reed LJ, Muench H (1938) A simple method of estimating fifty percent endpoints. Am J Epidemiol 27:493–497

30.

Shi WF, Gibbs MJ, Zhang YZ, Zhang Z, Zhao XM, Jin X, Zhu CD, Yang MF, Yang NN, Cui YJ, Ji L (2008) Genetic analysis of four porcine avian influenza viruses isolated from Shandong, China. Arch Virol 153:211–217PubMedCrossRef

31.

Smith GJ, Vijaykrishna D, Bahl J, Lycett SJ, Worobey M, Pybus OG, Ma SK, Cheung CL, Raghwani J, Bhatt S, Peiris JS, Guan Y, Rambaut A (2009) Origins and evolutionary genomics of the 2009 swine-origin H1N1 influenza A epidemic. Nature 459:1122–1125PubMedCrossRef

32.

Subbarao K, Klimov A, Katz J, Regnery H, Lim W, Hall H, Perdue M, Swayne D, Bender C, Huang J, Hemphill M, Rowe T, Shaw M, Xu X, Fukuda K, Cox N (1998) Characterization of an avian influenza A (H5N1) virus isolated from a child with a fatal respiratory illness. Science 279:393–396PubMedCrossRef

33.

Suptawiwat O, Kongchanagul A, Chan-It W, Thitithanyanont A, Wiriyarat W, Chaichuen K, Songserm T, Suzuki Y, Puthavathana P, Auewarakul P (2008) A simple screening assay for receptor switching of avian influenza viruses. J Clin Virol 42:186–189PubMedCrossRef

34.

Vijaykrishna D, Smith GJ, Pybus OG, Zhu H, Bhatt S, Poon LL, Riley S, Bahl J, Ma SK, Cheung CL, Perera RA, Chen H, Shortridge KF, Webby RJ, Webster RG, Guan Y, Peiris JS (2011) Long-term evolution and transmission dynamics of swine influenza A virus. Nature 473:519–522PubMedCrossRef

35.

Wan XF, Dong LB, Lan Y, Long LP, Xu CL, Zou SM, Li Z, Wen LY, Cai ZP, Wang W, Li XD, Yuan F, Sui HT, Zhang Y, Dong J, Sun SH, Gao Y, Wang M, Bai T, Yang L, Li DX, Yang WZ, Yu HJ, Wang SW, Feng ZJ, Wang Y, Guo YJ, Webby RJ, Shu YL (2011) Indications that live poultry markets are a major source of human H5N1 influenza virus infection in China. J Virol 85:13432–13438PubMedCrossRef

36.

Xu XY, Subbarao K, Cox NJ, Guo YJ (1999) Genetic characterization of the pathogenic influenza A/Goose/Guangdong/1/96 (H5N1) virus: similarity of its hemagglutinin gene to those of H5N1 viruses from the 1997 outbreaks in Hong Kong. Virology 261:15–19PubMedCrossRef

37.

Yamamoto Y, Nakamura K, Yamada M, Mase M (2012) Limited susceptibility of pigeons experimentally inoculated with H5N1 highly pathogenic avian influenza viruses. J Vet Med Sci 74:205–208PubMedCrossRef

38.

Yee KS, Carpenter TE, Cardona CJ (2009) Epidemiology of H5N1 avian influenza. Comp Immunol Microb 32:325–340CrossRef

39.

Yen HL, Lipatov AS, Ilyushina NA, Govorkova EA, Franks J, Yilmaz N, Douglas A, Hay A, Krauss S, Rehg JE, Hoffmann E, Webster RG (2007) Inefficient transmission of H5N1 influenza viruses in a ferret contact model. J Virol 81:6890–6898PubMedCrossRef

40.

Zhang Y, Sun YP, Sun HL, Pu J, Bi YH, Shi Y, Lu XS, Li J, Zhu QY, Gao GF, Yang HC, Liu JH (2012) A single amino acid at the hemagglutinin cleavage site contributes to the pathogenicity and neurovirulence of H5N1 influenza virus in mice. J Virol 86:6924–6931PubMedCrossRef

41.

Zhao G, Fan QP, Zhong L, Li YF, Liu WB, Liu XW, Gao S, Peng DX, Liu XF (2012) Isolation and phylogenetic analysis of pandemic H1N1/09 influenza virus from swine in Jiangsu province of China. Res Vet Sci 93:125–132PubMedCrossRef

42.

Zhu Q, Yang H, Chen W, Cao W, Zhong G, Jiao P, Deng G, Yu K, Yang C, Bu Z, Kawaoka Y, Chen H (2008) A naturally occurring deletion in its NS gene contributes to the attenuation of an H5N1 swine influenza virus in chickens. J Virol 82:220–228PubMedCrossRef

Titel: Isolation and characterization of two H5N1 influenza viruses from swine in Jiangsu Province of China
verfasst von: Liang He
Guo Zhao
Lei Zhong
Qingtao Liu
Zhiqiang Duan
Min Gu
Xiaoquan Wang
Xiaowen Liu
Xiufan Liu
Publikationsdatum: 01.12.2013
Verlag: Springer Vienna
Erschienen in: Archives of Virology / Ausgabe 12/2013
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-013-1771-y

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Isolation and characterization of two H5N1 influenza viruses from swine in Jiangsu Province of China

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