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Erschienen in: Archives of Virology 12/2014

01.12.2014 | Original Article

miR-125b inhibits hepatitis B virus expression in vitro through targeting of the SCNN1A gene

verfasst von: Zhenzhen Zhang, Juan Chen, Yin He, Xue Zhan, Ruiqiu Zhao, Yanfeng Huang, Hongmei Xu, Zhaomin Zhu, Quanbo Liu

Erschienen in: Archives of Virology | Ausgabe 12/2014

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Abstract

microRNAs (miRNAs) are small noncoding RNAs that modulate gene expression at the posttranscriptional level, playing an important role in many diseases. However, reports concerning the role of miRNA in hepatitis B virus (HBV) infection are limited. miRNA chips were used to investigate miRNA changes during HBV infection in vitro. Bioinformatics analysis was used to explore possible miRNA and target genes during HBV infection. The expression of miR-125b and its potential target gene, sodium channel, non-voltage-gated 1 alpha (SCNN1A), was further analyzed. A total of 136 miRNAs were analyzed in an HBV transient transfection model (HepG2-HBV1.3), and 78 miRNAs were differentially expressed in HepG2.2.15 cells compared with HepG2 cells. miR-125b expression was decreased in both HepG2-HBV1.3 and HepG2.2.15 cells, and ectopic expression of miR-125b inhibited HBV DNA intermediates and secretion of HBsAg and HBeAg. miR-125b also inhibited the mRNA and protein levels of SCNN1A. Using a dual luciferase reporter system, we found that SCNN1A was one of the targets of miR-125b. In this study, we found that miR-125b inhibits HBV expression in vitro by regulating SCNN1A expression.
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Literatur
1.
Zurück zum Zitat Andreas S, Philippe G, Stephan U (2007) Hepatitis B virus infection initiates with a large surface protein-dependent binding to heparin sulfate proteoglycans. Hepatology 46:1759–1768CrossRef Andreas S, Philippe G, Stephan U (2007) Hepatitis B virus infection initiates with a large surface protein-dependent binding to heparin sulfate proteoglycans. Hepatology 46:1759–1768CrossRef
2.
Zurück zum Zitat Zoulim F, Locarnini S (2009) Hepatitis B virus resistance to nucleos(t)ide analogues. Gastroenterology 137(5):1593–1608PubMedCrossRef Zoulim F, Locarnini S (2009) Hepatitis B virus resistance to nucleos(t)ide analogues. Gastroenterology 137(5):1593–1608PubMedCrossRef
3.
Zurück zum Zitat Pei S, Zhang Y, Xu H, Chen X, Chen S (2011) Inhibition of the replication of hepatitis B virus in vitro by pu-erh tea extracts. J Agric Food Chem 59:9927–9934PubMedCrossRef Pei S, Zhang Y, Xu H, Chen X, Chen S (2011) Inhibition of the replication of hepatitis B virus in vitro by pu-erh tea extracts. J Agric Food Chem 59:9927–9934PubMedCrossRef
4.
Zurück zum Zitat Liu C, Wang Y, Wu C, Pei R, Song J, Chen S, Chen X (2013) Dioscin’s antiviral effect in vitro. Virus Res 172:9–14PubMedCrossRef Liu C, Wang Y, Wu C, Pei R, Song J, Chen S, Chen X (2013) Dioscin’s antiviral effect in vitro. Virus Res 172:9–14PubMedCrossRef
5.
Zurück zum Zitat Bartel DP (2004) MicroRNAs: genomics, biogenesis, mechanism, and function. Cell 116:281–297PubMedCrossRef Bartel DP (2004) MicroRNAs: genomics, biogenesis, mechanism, and function. Cell 116:281–297PubMedCrossRef
6.
Zurück zum Zitat Berkhout B, Jeang KT (2007) RISCy business: MicroRNAs, pathogenesis, and viruses. J Biol Chem 282:26641–26645PubMedCrossRef Berkhout B, Jeang KT (2007) RISCy business: MicroRNAs, pathogenesis, and viruses. J Biol Chem 282:26641–26645PubMedCrossRef
9.
Zurück zum Zitat Pan Xiao-Ben, Ma Hui, Jin Qian, Wei Lai (2012) Characterization of microRNA expression profiles associated with hepatitis B virus replication and clearance in vivo and in vitro. J Gastroenterol Hepatol 27:805–812PubMedCrossRef Pan Xiao-Ben, Ma Hui, Jin Qian, Wei Lai (2012) Characterization of microRNA expression profiles associated with hepatitis B virus replication and clearance in vivo and in vitro. J Gastroenterol Hepatol 27:805–812PubMedCrossRef
10.
Zurück zum Zitat Jin W, Wang Y, Chen L, Wu E, Ye X, Gao GF, Wang F, Chen Y, Duan Z, Meng S (2012) Loss of microRNA122 expression in patients with hepatitis B enhances hepatitis B virus replication through cyclin G(1) -modulated P53 activity. Hepatology 55(3):730–741PubMedCrossRef Jin W, Wang Y, Chen L, Wu E, Ye X, Gao GF, Wang F, Chen Y, Duan Z, Meng S (2012) Loss of microRNA122 expression in patients with hepatitis B enhances hepatitis B virus replication through cyclin G(1) -modulated P53 activity. Hepatology 55(3):730–741PubMedCrossRef
11.
Zurück zum Zitat Fan CG, Wang CM, Tian C, Wang Y, Li L, Sun WS, Li RF, Liu YG (2011) miR-122 inhibits viral replication and cell proliferation in hepatitis B virus-related hepatocellular carcinoma and targets NDRG3. Oncol Rep 26(5):1281–1286PubMed Fan CG, Wang CM, Tian C, Wang Y, Li L, Sun WS, Li RF, Liu YG (2011) miR-122 inhibits viral replication and cell proliferation in hepatitis B virus-related hepatocellular carcinoma and targets NDRG3. Oncol Rep 26(5):1281–1286PubMed
12.
Zurück zum Zitat Potenza Nicoletta, Papa Umberto, Mosca Nicola, Zerbini Francesca, Nobile Valentina, Russo Aniello (2011) Human microRNA hsa-miR-125a-5p interferes with expression of hepatitis B virus surface antigen. Nucleic Acids Res 39:5157–5163PubMedCentralPubMedCrossRef Potenza Nicoletta, Papa Umberto, Mosca Nicola, Zerbini Francesca, Nobile Valentina, Russo Aniello (2011) Human microRNA hsa-miR-125a-5p interferes with expression of hepatitis B virus surface antigen. Nucleic Acids Res 39:5157–5163PubMedCentralPubMedCrossRef
13.
Zurück zum Zitat Hu W, Wang X, Ding X, Li Y, Zhang X, Xie P, Yang J, Wang S (2012) MicroRNA-141 represses HBV replication by targeting PPARA. PLoS One 7(3):e34165PubMedCentralPubMedCrossRef Hu W, Wang X, Ding X, Li Y, Zhang X, Xie P, Yang J, Wang S (2012) MicroRNA-141 represses HBV replication by targeting PPARA. PLoS One 7(3):e34165PubMedCentralPubMedCrossRef
14.
Zurück zum Zitat Huang Jinfeng, Wang Yue, Guo Yingjun, Sun Shuhan (2010) Down-regulated MicroRNA-152 induces aberrant DNA methylation in hepatitis B virus-related hepatocellular carcinoma by targeting DNA methyltransferase 1. Hepatology 52:1 Huang Jinfeng, Wang Yue, Guo Yingjun, Sun Shuhan (2010) Down-regulated MicroRNA-152 induces aberrant DNA methylation in hepatitis B virus-related hepatocellular carcinoma by targeting DNA methyltransferase 1. Hepatology 52:1
15.
Zurück zum Zitat Weber A, Marquardt J, Elzi D, Forster N, Starke S, Glaum A, Yamada D, Defossez PA, Delrow J, Eisenman RN, Christiansen H, Eilers M (2008) Zbtb4 represses transcription of P21CIP1 and controls the cellular response to p53 activation. EMBO J 27(11):1563–1574PubMedCentralPubMedCrossRef Weber A, Marquardt J, Elzi D, Forster N, Starke S, Glaum A, Yamada D, Defossez PA, Delrow J, Eisenman RN, Christiansen H, Eilers M (2008) Zbtb4 represses transcription of P21CIP1 and controls the cellular response to p53 activation. EMBO J 27(11):1563–1574PubMedCentralPubMedCrossRef
16.
Zurück zum Zitat Kim K, Chadalapaka G, Pathi SS, Jin UH, Lee JS, Park YY, Cho SG, Chintharlapalli S, Safe S (2012) Induction of the transcriptional repressor ZBTB4 in prostate cancer cells by drug-induced targeting of MicroRNA-17-92/106b-25 clusters. Mol Cancer Ther 11(9):1852–1862PubMedCentralPubMedCrossRef Kim K, Chadalapaka G, Pathi SS, Jin UH, Lee JS, Park YY, Cho SG, Chintharlapalli S, Safe S (2012) Induction of the transcriptional repressor ZBTB4 in prostate cancer cells by drug-induced targeting of MicroRNA-17-92/106b-25 clusters. Mol Cancer Ther 11(9):1852–1862PubMedCentralPubMedCrossRef
17.
Zurück zum Zitat Chenhe Su, Hou Zhaohua, Zhang Cai, Tian Zhigang, Zhang Jian (2011) Ectopic expression of microRNA-155 enhances innate antiviral immunity against HBV infection in human hepatoma cells. Virology 8:354CrossRef Chenhe Su, Hou Zhaohua, Zhang Cai, Tian Zhigang, Zhang Jian (2011) Ectopic expression of microRNA-155 enhances innate antiviral immunity against HBV infection in human hepatoma cells. Virology 8:354CrossRef
18.
Zurück zum Zitat Yang Lian, Ma Zhaolong, Wang Dongdong, Zhao Wenyue, Chen Libo, Wang Guobin (2010) MicroRNA-602 regulating tumor suppressive gene RASSF1A is over-expressed in hepatitis B virus-infected liver and hepatocellular carcinoma. Cancer Biol Ther 9(10):803–808PubMedCrossRef Yang Lian, Ma Zhaolong, Wang Dongdong, Zhao Wenyue, Chen Libo, Wang Guobin (2010) MicroRNA-602 regulating tumor suppressive gene RASSF1A is over-expressed in hepatitis B virus-infected liver and hepatocellular carcinoma. Cancer Biol Ther 9(10):803–808PubMedCrossRef
19.
Zurück zum Zitat Huang J, Wang F, Argyris E, Chen K, Liang Z, Tian H, Huang W, Squires K, Verlinghieri G, Zhang H (2007) Cellular microRNAs contribute to HIV-1 latency in resting primary CD4+T lymphocytes. Nat. Med 13:1241–1247PubMedCrossRef Huang J, Wang F, Argyris E, Chen K, Liang Z, Tian H, Huang W, Squires K, Verlinghieri G, Zhang H (2007) Cellular microRNAs contribute to HIV-1 latency in resting primary CD4+T lymphocytes. Nat. Med 13:1241–1247PubMedCrossRef
20.
Zurück zum Zitat Nuovo GJ, Wu X, Volinia S, Yan F, di Leva G, Chin N, Nicol AF, Jiang J, Otterson G, Schmittgen TD, Croce C (2010) Strong inverse correlation between microRNA-125b and human papillomavirus DNA in productive infection. Diagn Mol Pathol 19:135–143PubMedCrossRef Nuovo GJ, Wu X, Volinia S, Yan F, di Leva G, Chin N, Nicol AF, Jiang J, Otterson G, Schmittgen TD, Croce C (2010) Strong inverse correlation between microRNA-125b and human papillomavirus DNA in productive infection. Diagn Mol Pathol 19:135–143PubMedCrossRef
21.
Zurück zum Zitat Jia HY, Wang YX, Yan WT, Li HY, Tian YZ, Wang SM, Zhao HL (2012) MicroRNA-125b functions as a tumor suppressor in hepatocellular carcinoma cells. Int J Mol Sci 13(7):8762–8774PubMedCentralPubMedCrossRef Jia HY, Wang YX, Yan WT, Li HY, Tian YZ, Wang SM, Zhao HL (2012) MicroRNA-125b functions as a tumor suppressor in hepatocellular carcinoma cells. Int J Mol Sci 13(7):8762–8774PubMedCentralPubMedCrossRef
22.
23.
Zurück zum Zitat Kellenberger S, Schild L (2002) Epithelial sodium channel/degenerin family of ion channels: a variety of functions for a shared structure. Physiol Rev 82:735–767PubMed Kellenberger S, Schild L (2002) Epithelial sodium channel/degenerin family of ion channels: a variety of functions for a shared structure. Physiol Rev 82:735–767PubMed
24.
Zurück zum Zitat Dirlewanger M, Huser D, Zennaro MC, Girardin E, Schild L, Schwitzgebel VM (2011) A homozygous missense mutation in SCNN1A is responsible for a transient neonatal form of pseudohypoaldosteronism type 1. Am J Physiol Endocrinol, Meta 301(3):467–473CrossRef Dirlewanger M, Huser D, Zennaro MC, Girardin E, Schild L, Schwitzgebel VM (2011) A homozygous missense mutation in SCNN1A is responsible for a transient neonatal form of pseudohypoaldosteronism type 1. Am J Physiol Endocrinol, Meta 301(3):467–473CrossRef
Metadaten
Titel
miR-125b inhibits hepatitis B virus expression in vitro through targeting of the SCNN1A gene
verfasst von
Zhenzhen Zhang
Juan Chen
Yin He
Xue Zhan
Ruiqiu Zhao
Yanfeng Huang
Hongmei Xu
Zhaomin Zhu
Quanbo Liu
Publikationsdatum
01.12.2014
Verlag
Springer Vienna
Erschienen in
Archives of Virology / Ausgabe 12/2014
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-014-2208-y

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