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Erschienen in: Archives of Virology 6/2015

01.06.2015 | Original Article

A study of lymphoid organs and serum proinflammatory cytokines in pigs infected with African swine fever virus genotype II

verfasst von: Hovakim Zakaryan, Victorya Cholakyans, Lusine Simonyan, Alla Misakyan, Elena Karalova, Andranik Chavushyan, Zaven Karalyan

Erschienen in: Archives of Virology | Ausgabe 6/2015

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Abstract

African swine fever virus (ASFV), the causative agent of one of the most important viral diseases of domestic pigs for which no vaccine is available, causes immune system disorders in infected animals. In this study, the serum levels of proinflammatory cytokines, as well as the histological and cellular constitution of lymphoid organs of pigs infected with ASFV genotype II were investigated. The results showed a high degree of lymphocyte depletion in the lymphoid organs, particularly in the spleen and lymph nodes, where ASFV infection led to a twofold decrease in the number of lymphocytes on the final day of infection. Additionally, ASFV-infected pigs had atypical forms of lymphocytes found in all lymphoid organs. In contrast to lymphocytes, the number of immature immune cells, particularly myelocytes, increased dramatically and reached a maximum on day 7 postinfection. The serum levels of TNF-α, IL-1β, IL-6, and IL-8 were evaluated. Proinflammatory cytokines showed increased levels after ASFV infection, with peak values at 7 days postinfection, and this highlights their role in the pathogenesis of ASFV. In conclusion, this study showed that ASFV genotype II, like other highly virulent strains, causes severe pathological changes in the immune system of pigs.
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Metadaten
Titel
A study of lymphoid organs and serum proinflammatory cytokines in pigs infected with African swine fever virus genotype II
verfasst von
Hovakim Zakaryan
Victorya Cholakyans
Lusine Simonyan
Alla Misakyan
Elena Karalova
Andranik Chavushyan
Zaven Karalyan
Publikationsdatum
01.06.2015
Verlag
Springer Vienna
Erschienen in
Archives of Virology / Ausgabe 6/2015
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-015-2401-7

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