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Archives of Virology

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01.03.2016 | Brief Report

High-yield production of canine parvovirus virus-like particles in a baculovirus expression system

verfasst von: Hongli Jin, Xiaohong Xia, Bing Liu, Yu Fu, Xianping Chen, Huihui Wang, Zhenqiang Xia

Erschienen in: Archives of Virology | Ausgabe 3/2016

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Abstract

An optimized VP2 gene from the current prevalent CPV strain (new CPV-2a) in China was expressed in a baculovirus expression system. It was found that the VP2 proteins assembled into virus-like particles (VLPs) with antigenic properties similar to those of natural CPV and with an especially high hemagglutination (HA) titer (1:2²⁰). Dogs intramuscularly or orally immunized with VLPs produced antibodies against CPV with >1:80 hemagglutination inhibition (HI) units for at least 3 months. The CPV VLPs could be considered for use as a vaccine against CPV or as a platform for research on chimeric VLP vaccines against other diseases.

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Titel: High-yield production of canine parvovirus virus-like particles in a baculovirus expression system
verfasst von: Hongli Jin
Xiaohong Xia
Bing Liu
Yu Fu
Xianping Chen
Huihui Wang
Zhenqiang Xia
Publikationsdatum: 01.03.2016
Verlag: Springer Vienna
Erschienen in: Archives of Virology / Ausgabe 3/2016
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-015-2719-1

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High-yield production of canine parvovirus virus-like particles in a baculovirus expression system

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