Erschienen in:
01.03.2016 | Brief Report
High-yield production of canine parvovirus virus-like particles in a baculovirus expression system
verfasst von:
Hongli Jin, Xiaohong Xia, Bing Liu, Yu Fu, Xianping Chen, Huihui Wang, Zhenqiang Xia
Erschienen in:
Archives of Virology
|
Ausgabe 3/2016
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Abstract
An optimized VP2 gene from the current prevalent CPV strain (new CPV-2a) in China was expressed in a baculovirus expression system. It was found that the VP2 proteins assembled into virus-like particles (VLPs) with antigenic properties similar to those of natural CPV and with an especially high hemagglutination (HA) titer (1:220). Dogs intramuscularly or orally immunized with VLPs produced antibodies against CPV with >1:80 hemagglutination inhibition (HI) units for at least 3 months. The CPV VLPs could be considered for use as a vaccine against CPV or as a platform for research on chimeric VLP vaccines against other diseases.