nach oben

Archives of Virology

Erschienen in:

05.01.2016 | Brief Report

Rapid and sensitive detection of canine parvovirus type 2 by recombinase polymerase amplification

verfasst von: Jianchang Wang, Libing Liu, Ruiwen Li, Jinfeng Wang, Qi Fu, Wanzhe Yuan

Erschienen in: Archives of Virology | Ausgabe 4/2016

Einloggen, um Zugang zu erhalten

Abstract

A novel recombinase polymerase amplification (RPA)-based method for detection of canine parvovirus type 2 (CPV-2) was developed. Sensitivity analysis showed that the detection limit of RPA was 10 copies of CPV-2 genomic DNA. RPA amplified both CPV-2a and -2b DNA but did not amplify the template of other important dog viruses (CCoV, PRV or CDV), demonstrating high specificity. The method was further validated with 57 canine fecal samples. An outstanding advantage of RPA is that it is an isothermal reaction and can be performed in a water bath, making RPA a potential alternative method for CPV-2 detection in resource-limited settings.

Appel MJ, Scott FW, Carmichael LE (1979) Isolation and immunisation studies of a canine parco-like virus from dogs with haemorrhagic enteritis. Vet Rec 105:156–159CrossRefPubMed

Hoelzer K, Parrish CR (2010) The emergence of parvoviruses of carnivores. Vet Res 41:39CrossRefPubMedPubMedCentral

Desario C, Decaro N, Campolo M, Cavalli A, Cirone F, Elia G, Martella V, Lorusso E, Camero M, Buonavoglia C (2005) Canine parvovirus infection: which diagnostic test for virus? J Virol Methods 126:179–185CrossRefPubMed

Macartney L, McCandlish IA, Thompson H, Cornwell HJ (1984) Canine parvovirus enteritis 1: clinical, haematological and pathological features of experimental infection. Vet Rec 115:201–210CrossRefPubMed

Decaro N, Desario C, Campolo M, Elia G, Martella V, Ricci D, Lorusso E, Buonavoglia C (2005) Clinical and virological findings in pups naturally infected by canine parvovirus type 2 Glu-426 mutant. J Vet Diagn Invest 17:133–138CrossRefPubMed

Uwatoko K, Sunairi M, Nakajima M, Yamaura K (1995) Rapid method utilizing the polymerase chain reaction for detection of canine parvovirus in feces of diarrheic dogs. Vet Microbiol 43:315–323CrossRefPubMed

Hirasawa T, Kaneshige T, Mikazuki K (1994) Sensitive detection of canine parvovirus DNA by the nested polymerase chain reaction. Vet Microbiol 41:135–145CrossRefPubMed

Kumar M, Nandi S (2010) Development of a SYBR Green based real-time PCR assay for detection and quantitation of canine parvovirus in faecal samples. J Virol Methods 169:198–201CrossRefPubMed

Sun YL, Yen CH, Tu CF (2014) Visual detection of canine parvovirus based on loop-mediated isothermal amplification combined with enzyme-linked immunosorbent assay and with lateral flow dipstick. J Vet Med Sci 76:509–516CrossRefPubMedPubMedCentral

10.

Wilkes RP, Lee PY, Tsai YL, Tsai CF, Chang HH, Chang HF, Wang HT (2015) An insulated isothermal PCR method on a field-deployable device for rapid and sensitive detection of canine parvovirus type 2 at points of need. J Virol Methods 220:35–38CrossRefPubMed

11.

Piepenburg O, Williams CH, Stemple DL, Armes NA (2006) DNA detection using recombination proteins. PLoS Biol 4:e204CrossRefPubMedPubMedCentral

12.

Boyle DS, McNerney R, Teng Low H, Leader BT, Perez-Osorio AC, Meyer JC, O’Sullivan DM, Brooks DG, Piepenburg O, Forrest MS (2014) Rapid detection of Mycobacterium tuberculosis by recombinase polymerase amplification. PLoS One 9:e103091CrossRefPubMedPubMedCentral

13.

Sakai K, Trabasso P, Moretti ML, Mikami Y, Kamei K, Gonoi T (2014) Identification of fungal pathogens by visible microarray system in combination with isothermal gene amplification. Mycopathologia 178:11–26CrossRefPubMedPubMedCentral

14.

Crannell ZA, Cabada MM, Castellanos-Gonzalez A, Irani A, White AC, Richards-Kortum R (2014) Recombinase polymerase amplification-based assay to diagnose Giardia in stool samples. Am J Trop Med Hyg 92:583–587CrossRefPubMed

15.

Boyle DS, Lehman DA, Lillis L, Peterson D, Singhal M, Armes N, Parker M, Piepenburg O, Overbaugh J (2013) Rapid detection of HIV-1 proviral DNA for early infant diagnosis using recombinase polymerase amplification. MBio 4:e00135-13CrossRefPubMedPubMedCentral

16.

Abd EI, Wahed A, El-Deeb A, El-Tholoth M, Abd El Kader H, Ahmed A, Hassan S, Hoffmann B, Haas B, Shalaby MA, Hufert FT, Weidmann M (2013) A portable reverse transcription recombinase polymerase amplification assay for rapid detection of foot-and-mouth disease virus. PLoS One 8:e71642CrossRef

17.

Zaghloul H, El-shahat M (2014) Recombinase polymerase amplification as a promising tool in hepatitis C virus diagnosis. World J Hepatol 6:916–922CrossRefPubMedPubMedCentral

Titel: Rapid and sensitive detection of canine parvovirus type 2 by recombinase polymerase amplification
verfasst von: Jianchang Wang
Libing Liu
Ruiwen Li
Jinfeng Wang
Qi Fu
Wanzhe Yuan
Publikationsdatum: 05.01.2016
Verlag: Springer Vienna
Erschienen in: Archives of Virology / Ausgabe 4/2016
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-015-2738-y

Leitlinien kompakt für die Innere Medizin

Mit medbee Pocketcards sicher entscheiden.

^{Seit 2022 gehört die medbee GmbH zum Springer Medizin Verlag}

Kostenlos registrieren

Neu im Fachgebiet Innere Medizin

25.04.2024 | Hypotonie | Nachrichten

Update Innere Medizin

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert.

Newsletter bestellen

Springer Medizin

Rapid and sensitive detection of canine parvovirus type 2 by recombinase polymerase amplification

Abstract

Leitlinien kompakt für die Innere Medizin

Neu im Fachgebiet Innere Medizin

Niedriger diastolischer Blutdruck erhöht Risiko für schwere kardiovaskuläre Komplikationen

Therapiestart mit Blutdrucksenkern erhöht Frakturrisiko

Adjuvante Immuntherapie verlängert Leben bei RCC

Alectinib verbessert krankheitsfreies Überleben bei ALK-positivem NSCLC

Update Innere Medizin

Springer Medizin

Abstract

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 4/2016

Molecular characterization of a novel capillovirus from red currant

Genetic heterogeneity of the VP6 gene and predominance of G6P[5] genotypes of Brazilian porcine rotavirus C field strains

An outbreak of encephalitis associated with echovirus 19 in Uttar Pradesh, India, in 2011

Detection and genetic characterization of a novel parvovirus distantly related to human bufavirus in domestic pigs

A critical role of LAMP-1 in avian reovirus P10 degradation associated with inhibition of apoptosis and virus release

Development and characterization of a synthetic infectious cDNA clone of the virulent Bucyrus strain of equine arteritis virus expressing mCherry (red fluorescent protein)

Leitlinien kompakt für die Innere Medizin

Neu im Fachgebiet Innere Medizin

Niedriger diastolischer Blutdruck erhöht Risiko für schwere kardiovaskuläre Komplikationen

Therapiestart mit Blutdrucksenkern erhöht Frakturrisiko

Adjuvante Immuntherapie verlängert Leben bei RCC

Alectinib verbessert krankheitsfreies Überleben bei ALK-positivem NSCLC

Update Innere Medizin