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Erschienen in: Archives of Virology 6/2016

03.03.2016 | Brief Report

Characterization and occurrence of squash chlorotic leaf spot virus, a tentative new torradovirus infecting cucurbits in Sudan

verfasst von: H. Lecoq, E. Verdin, M. Tepfer, C. Wipf-Scheibel, P. Millot, G. Dafalla, C. Desbiez

Erschienen in: Archives of Virology | Ausgabe 6/2016

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Abstract

During a survey conducted in Sudan in 2012, a virus with spherical particles was isolated from a squash plant showing chlorotic leaf spots. The virus was transmitted mechanically and by two whitefly species, but not by aphids. RT-PCR with generic torradovirus primers yielded a band of expected size from total RNA of a symptomatic plant. Next-generation sequencing confirmed that this is tentatively a new torradovirus, for which we propose the name ‘squash chlorotic leaf spot virus’. Using specific RT-PCR primers, the virus was detected in cucurbit samples collected since 1992 at different locations in Sudan.
Literatur
1.
Zurück zum Zitat Lecoq H (2003) Cucurbits. In: Loebenstein G, Thottapilly G (eds) Virus and virus-like diseases of major crops in developing countries. Kluwer, Dordrecht, pp 665–688CrossRef Lecoq H (2003) Cucurbits. In: Loebenstein G, Thottapilly G (eds) Virus and virus-like diseases of major crops in developing countries. Kluwer, Dordrecht, pp 665–688CrossRef
2.
Zurück zum Zitat Lecoq H, Dafalla GA, Mohamed YF, Ali HM, Wipf-Scheibel C, Desbiez C, Eljack A, Omara SK, Pitrat M (1994) Survey of virus diseases infecting cucurbit crops in Eastern, Central and Western Sudan. Univ Khartoum J Agric Sci 2:67–82 Lecoq H, Dafalla GA, Mohamed YF, Ali HM, Wipf-Scheibel C, Desbiez C, Eljack A, Omara SK, Pitrat M (1994) Survey of virus diseases infecting cucurbit crops in Eastern, Central and Western Sudan. Univ Khartoum J Agric Sci 2:67–82
3.
Zurück zum Zitat Lecoq H, Dafalla G, Delécolle B, Wipf-Scheibel C, Desbiez C (2011) Snake melon asteroid mosaic virus, a tentative new member of the genus Sobemovirus infecting cucurbits. Plant Dis 95:153–157CrossRef Lecoq H, Dafalla G, Delécolle B, Wipf-Scheibel C, Desbiez C (2011) Snake melon asteroid mosaic virus, a tentative new member of the genus Sobemovirus infecting cucurbits. Plant Dis 95:153–157CrossRef
4.
Zurück zum Zitat Mahgoub HA, Desbiez C, Wipf-Scheibel C, Dafalla G, Lecoq H (1997) Characterization and occurrence of zucchini yellow mosaic virus in Sudan. Plant Pathol 46:800–805CrossRef Mahgoub HA, Desbiez C, Wipf-Scheibel C, Dafalla G, Lecoq H (1997) Characterization and occurrence of zucchini yellow mosaic virus in Sudan. Plant Pathol 46:800–805CrossRef
5.
Zurück zum Zitat Mohammed HS, Zicca S, Manglli A, Mohamed ME, El Siddig MA, Tomassoli L, El Hussein AA (2014) Identification and phylogenetic analysis of common pumpkin viruses in Sudan. J Plant Pathol 96:77–84 Mohammed HS, Zicca S, Manglli A, Mohamed ME, El Siddig MA, Tomassoli L, El Hussein AA (2014) Identification and phylogenetic analysis of common pumpkin viruses in Sudan. J Plant Pathol 96:77–84
6.
Zurück zum Zitat Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739CrossRefPubMedPubMedCentral Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739CrossRefPubMedPubMedCentral
7.
Zurück zum Zitat Van der Vlugt RAA, Verbeek M, Dullemans AM, Wintermantel WM, Cuellar WJ, Fox A, Thompson JR (2015) Torradoviruses. Ann Rev Phytopathol 53:485–512CrossRef Van der Vlugt RAA, Verbeek M, Dullemans AM, Wintermantel WM, Cuellar WJ, Fox A, Thompson JR (2015) Torradoviruses. Ann Rev Phytopathol 53:485–512CrossRef
8.
Zurück zum Zitat Verbeek M, Tang J, Ward LI (2012) Two generic PCR primer sets for the detection of members of genus Torradovirus. J Virol Methods 185:184–188CrossRefPubMed Verbeek M, Tang J, Ward LI (2012) Two generic PCR primer sets for the detection of members of genus Torradovirus. J Virol Methods 185:184–188CrossRefPubMed
Metadaten
Titel
Characterization and occurrence of squash chlorotic leaf spot virus, a tentative new torradovirus infecting cucurbits in Sudan
verfasst von
H. Lecoq
E. Verdin
M. Tepfer
C. Wipf-Scheibel
P. Millot
G. Dafalla
C. Desbiez
Publikationsdatum
03.03.2016
Verlag
Springer Vienna
Erschienen in
Archives of Virology / Ausgabe 6/2016
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-016-2797-8

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