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24.09.2016 | Original Article

Seronegative and occult hepatitis C virus infections in patients with hematological disorders

verfasst von: Ghada Fahmy Helaly, Amel Gaber Elsheredy, Adel Abd El Basset Mousa, Hayat Khalifa Fadlalla Ahmed, Abd El-Gaffar Sabry Oluyemi

Erschienen in: Archives of Virology | Ausgabe 1/2017

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Abstract

Studies of the association between seronegative or occult (OCI) hepatitis C virus (HCV) infection, and hematological disorders have yielded controversial results. The aim of this study was to investigate seronegative and OCI HCV infections in among patients with different hematological disorders. This study included 90 anti-HCV-negative patients with either benign or malignant hematological disorders (group I), along with 20 age- and sex-matched apparently healthy subjects, who served as controls (group II). We tested for HCV RNA in sera and PBMCs by RT-nested PCR and for liver enzyme activity. Seronegativity and OCI were detected in 66.7 % and 20 % respectively, of the studied cases (group I). OCI was more evident in Hodgkin lymphoma and thalassemia. A significant increase in AST activity was observed in the seronegative and OCI groups and in ALT and AST in HCV-seronegative or OCI and negative HCV patients (p ≤ 0.05). Seronegativity and OCI are a significant clinical problem in patients with hematological disorders, warranting wider use of molecular tests combined with periodic evaluations of liver functions for diagnostic purposes.

Sievert W, Altraif I, Razavi HA, Abdo A, Ahmed EA, Alomair A, Amarapurkar D, Chen CH, Dou X, El Khayat H, Elshazly M, Esmat G, Guan R, Han KH, Koike K, Largen A, McCaughan G, Mogawer S, Monis A, Nawaz A, Piratvisuth T, Sanai FM, Sharara AI, Sibbel S, Sood A, Suh DJ, Wallace C, Young K, Negro F (2011) A systematic review of hepatitis C virus epidemiology in Asia, Australia and Egypt. Liver Int 31(Suppl 2):61–80CrossRefPubMed

Carreno V, Bartolome J, Castillo I, Quiroga JA (2012) New perspectives in occult hepatitis C virus infection. World J Gastroenterol 18:2887–2894CrossRefPubMedPubMedCentral

Seeff LB (2002) Natural history of chronic hepatitis C. Hepatology 36:S35–S46CrossRefPubMed

Shepard CW, Finelli L, Alter MJ (2005) Global epidemiology of hepatitis C virus infection. Lancet Infect Dis 5:558–567CrossRefPubMed

Kaźmierczak J, Pawełczyk A, Cortes KC, Radkowski M (2014) Seronegative hepatitis C virus infection. Arch Immunol Ther Exp 62:145–151CrossRef

Stramer SL, Glynn SA, Kleinman SH, Strong DM, Caglioti S, Wright DJ, Dodd RY, Busch MP, National Heart, Lung, and Blood Institute Nucleic Acid Test Study Group (2004) Detection of HIV-1 and HCV infections among antibody-negative blood donors by nucleic acid-amplification testing. N Engl J Med. 351:760–768CrossRefPubMed

Tugwell BD, Patel PR, Williams IT, Hedberg K, Chai F, Nainan OV, Thomas AR, Woll JE, Bell BP, Cieslak PR (2005) Transmission of hepatitis C virus to several organ and tissue recipients from an antibody-negative donor. Ann Intern Med 143:648–654CrossRefPubMed

Thomson EC, Nastouli E, Main J, Karayiannis P, Eliahoo J, Muir D, McClure MO (2009) Delayed anti-HCV antibody response in HIV-positive men acutely infected with HCV. Aids 23:89–93CrossRefPubMedPubMedCentral

Castillo I, Rodriguez-Inigo E, Bartolome J, de Lucas S, Ortiz-Movilla N, Lopez-Alcorocho JM, Pardo M, Carreno V (2005) Hepatitis C virus replicates in peripheral blood mononuclear cells of patients with occult hepatitis C virus infection. Gut 54:682–685CrossRefPubMedPubMedCentral

10.

Castillo I, Rodrıguez-Inigo E, Lopez-Alcorocho JM, Pardo M, Bartolome J, Carreno V (2006) Hepatitis C virus replicates in the liver of patients who have a sustained response to antiviral treatment. Clin Infect Dis 43:1277–1283CrossRefPubMed

11.

Radkowski M, Horban A, Gallegos-Orozco JF, Pawelczyk A, Jablonska J, Wilkinson J, Adair D, Laskus T (2005) Evidence for viral persistence in patients who test positive for anti-hepatitis C virus antibodies and have normal alanine aminotransferase levels. J Infect Dis 191:1730–1733CrossRefPubMed

12.

Carreño V, Bartolomé J, Castillo I, Quiroga JA (2008) Occult hepatitis B virus and hepatitis C virus infections. Rev Med Virol 18:139–157CrossRefPubMed

13.

Castillo I, Bartolomé J, Quiroga JA, Barril G, Carreño V (2010) Diagnosis of occult hepatitis C without the need for a liver biopsy. J Med Virol 82:1554–1559CrossRefPubMed

14.

Kisiel E, Radkowski M, Pawelczyk A, Horban A, Stanczak J, Bukowska-Ośko I, Caraballo Cortes K, Kaźmierczak J, Popiel M, Laskus T (2014) Seronegative hepatitis C virus infection in patients with lymphoproliferative disorders. J Viral Hepat. 21:424–429CrossRefPubMed

15.

Farahani M, Bokharaei-Salim F, Ghane M, Basi A, Meysami P, Keyvani H (2013) Prevalence of occult hepatitis C virus infection in Iranian patients with lymphoproliferative disorders. J Med Virol. 85:235–240CrossRefPubMed

16.

Pawełczyk A, Polańska M, Kisiel E, Chmielewski M, Bukowska I, Fic M, Radkowski M (2009) Replication of HCV in bone marrow of patients with haematological disorders. Przegl Epidemiol 63:29–33PubMed

17.

Bastani MN, Bokharaei-Salim F, Keyvani H, Esghaei M, Monavari SH, Ebrahimi M, Garshasebi S, Fakhim S (2016) Prevalence of occult hepatitis C virus infection in Iranian patients with beta thalassemia major. Arch Virol 161:1899–1906CrossRefPubMed

18.

Mizutani T, Kato N, Saito S, Ikeda M, Sugiyama K, Shimotohno K (1996) Characterization of hepatitis C virus replication in cloned cells obtained from a human T-cell leukemia virus type 1-infected cell line, MT-2. J Virol 70:7219–7223PubMedPubMedCentral

19.

Quiroga JA, Castillo I, Pardo M, Rodriguez-Inigo E, Carreno V (2006) Combined hepatitis C virus (HCV) antigen-antibody detection assay does not improve diagnosis for seronegative individuals with occult HCV infection. J Clin Microbiol 44:4559–4560CrossRefPubMedPubMedCentral

20.

Zayed RA, Rushdy E, Saleh DA (2010) Detection of HCV RNA in the peripheral blood mononuclear cells of serum HCV RNA-negative Egyptian patients under interferon treatment. Am J Med Sci 340:435–438CrossRefPubMed

21.

Revie D, Salahuddin SZ (2011) Human cell types important for hepatitis C virus replication in vivo and in vitro: old assertions and current evidence. Virol J 8:346. doi:10.1186/1743-422X-8-346 CrossRefPubMedPubMedCentral

22.

Post JJ, Pan Y, Freeman AJ, Harvey CE, White PA, Palladinetti P, Haber PS, Marinos G, Levy MH, Kaldor JM, Dolan KA, Ffrench RA, Lloyd AR, Rawlinson WD, Hepatitis C Incidence and Transmission in Prisons Study (HITS) Group (2004) Clearance of hepatitis C viremia associated with cellular immunity in the absence of seroconversion in the hepatitis C incidence and transmission in prisons study cohort. J Infect Dis 189:1846–1855CrossRefPubMed

23.

Larouche A, Gaetan G, El-Bilali N, Quesnel-Vallieres M, Martin SR, Alvarez F, Shoukry NH, Soudeyns H (2012) Seronegative hepatitis C virus infection in a child infected via mother-to-child transmission. J Clin Microbiol 50:2515–2519CrossRefPubMedPubMedCentral

24.

Alnaqy A, Al-Harthy S, Kaminski G, Al-Dhahry S (2006) Detection of serum antibodies to hepatitis C virus in ‘false-seronegative’ blood donors in Oman. Med Princ Pract 15:111–113CrossRefPubMed

25.

Sarhan MA, Pham TN, Chen AY, Michalak TI (2012) Hepatitis C virus infection of human T lymphocytes is mediated by CD5. J Virol 86:3723–3735CrossRefPubMedPubMedCentral

26.

Bokharaei-Salim F, Keyvani H, Monavari SH, Alavian SM, Madjd Z, Toosi MN, Mohammad Alizadeh AH (2011) Occult hepatitis C virus infection in Iranian patients with cryptogenic liver disease. J Med Virol 83:989–995CrossRefPubMed

27.

Coppola N, Pisaturo M, Guastafierro S, Tonziello G, Sica A, Iodice V, Sagnelli C, Ferrara MG, Sagnelli E (2012) Increased hepatitis C viral load and reactivation of liver disease in HCV RNA-positive patients with onco-haematological disease undergoing chemotherapy. Dig Liver Dis 44:49–54CrossRefPubMed

28.

Sagnelli E, Pisaturo M, Sagnelli C, Coppola N (2012) Rituximab-based treatment, HCV replication and hepatic flares. Clin Dev Immunol 2012 (article ID 945950). doi:10.1155/2012/945950

29.

Pisaturo M, Guastafierro S, Filippini P, Tonziello G, Sica A, Di Martino F, Sagnelli C, Ferrara MG, Martini S, Cozzolino D, Sagnelli E, Coppola N (2013) Absence of occult HCV infection in patients experiencing an immunodepression condition. Infez Med 21:296–301PubMed

30.

Merat S, Rezvan H, Nouraie M, Jafari E, Abolghasemi H, Radmard AR, Zaer-rezaii H, Amini-Kafiabad S, Maghsudlu M, Pourshams A, Malekzadeh R, Esmaili S (2010) Seroprevalence of hepatitis C virus: the first population-based study from Iran. Int J Infect Dis 14(Suppl 3):e113–e116CrossRefPubMed

31.

Shaker O, Ahmed A, Abdel Satar I, El Ahl H, Shousha W, Doss W (2012) Occult hepatitis B in Egyptian thalassemic children. J Infect Dev Ctries 6:340–346CrossRefPubMed

32.

Youssef SS, Nasr AS, El Zanaty T, El Rawi RS, Mattar MM (2012) Prevalence of occult hepatitis C virus in Egyptian patients with chronic lymphoproliferative disorders. Hepat Res Treat 2012:429784. doi:10.1155/2012/429784 PubMedPubMedCentral

33.

Mansour AK, Zaki MES (2014) Non Hodgkin lymphoma and hepatitis C virus in Egyptian children: one centre study. J Hematol Res 1:69–74CrossRef

34.

Mohamoud YA, Mumtaz GR, Riome S, Miller D, Abu-Raddad LJ (2013) The epidemiology of hepatitis C virus in Egypt: a systematic review and data synthesis. BMC Infect Dis 13:288. doi:10.1186/1471-2334-13-288 CrossRefPubMedPubMedCentral

35.

Esaki T, Suzuki N, Yokoyama K, Iwata K, Irie M, Anan A, Nakane H, Yoshikane M, Nishizawa S, Ueda S, Sohda T, Watanabe H, Sakisaka S (2004) Hepatocellular carcinoma in a patient with liver cirrhosis associated with negative serum HCV tests but positive liver tissue HCV RNA. Intern Med 43:279–282CrossRefPubMed

36.

Comar M, Dal Molin G, D’Agaro P, Croce SL, Tiribelli C, Campello C (2006) HBV, HCV, and TTV detection by in situ polymerase chain reaction could reveal occult infection in hepatocellular carcinoma: comparison with blood markers. J Clin Pathol 59:526–529CrossRefPubMedPubMedCentral

37.

Cortés-Mancera FM, Restrepo JC, Osorio G, Hoyos S, Correa G, Navas MC (2010) Occult hepatitis C virus infection in a re-transplanted patient with liver failure of unknown etiology. Rev Colomb Gastroenterol 25:76–85

38.

Zaghloul H, El-Sherbiny W (2010) Detection of occult hepatitis C and hepatitis B virus infections from peripheral blood mononuclear cells. Immunol Invest 39:284–291CrossRefPubMed

39.

Idrees M, Lal A, Malik FA, Hussain A, Rehman I, Akbar H, Butt S, Ali M, Ali L, Malik FA (2011) Occult hepatitis C virus infection and associated predictive factors: the Pakistan experience. Infect Genet Evol 11:442–445CrossRefPubMed

Titel: Seronegative and occult hepatitis C virus infections in patients with hematological disorders
verfasst von: Ghada Fahmy Helaly
Amel Gaber Elsheredy
Adel Abd El Basset Mousa
Hayat Khalifa Fadlalla Ahmed
Abd El-Gaffar Sabry Oluyemi
Publikationsdatum: 24.09.2016
Verlag: Springer Vienna
Erschienen in: Archives of Virology / Ausgabe 1/2017
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-016-3049-7

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