Erschienen in:
18.03.2017 | Annotated Sequence Record
A novel vitivirus-like sequence found in Arracacia xanthorrhiza plants by high throughput sequencing
verfasst von:
Layssa M. Oliveira, Anelise F. Orílio, Alice K. Inoue-Nagata, Tatsuya Nagata, Rosana Blawid
Erschienen in:
Archives of Virology
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Ausgabe 7/2017
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Abstract
High throughput sequencing (HTS) is a very powerful tool for detecting and discovering novel viral-like sequences without prior knowledge of the sequence. Here we describe the complete genome of a new vitivirus-like sequence that was found in arracacha (Arracacia xanthorrhiza) plants using HTS technology. The complete genome sequence was validated by Sanger sequencing. The genomic organization of the new putative vitivirus resembles that of grapevine virus B (GVB) and grapevine virus D (GVD). The putative coat protein showed 41 to 49% identity with similar proteins of known vitiviruses, while the RNA-dependent RNA polymerase shared 52 to 55% identity with those encoded by grapevine vitiviruses. Based on the demarcation criteria for the genus Vitivirus, the virus described in this work, provisionally named as “Arracacha virus V”, represents a novel species in this taxon.