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Erschienen in: Archives of Virology 6/2018

01.03.2018 | Original Article

Characterization of the interactome of the porcine reproductive and respiratory syndrome virus glycoprotein-5

verfasst von: Maodong Zhang, Alexander Zakhartchouk

Erschienen in: Archives of Virology | Ausgabe 6/2018

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Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most important pathogens in the swine industry, causing reproductive failure in sows and respiratory disorders in piglets. Glycosylated protein 5 (GP5) is a major envelope protein of the virus. It is essential for virus particle assembly and involved in viral pathogenesis. In the present study, we identified the host cellular proteins that interact with GP5 by performing immunoprecipitation in MARC-145 cells infected by a recombinant PRRSV containing a FLAG-tag insertion in GP5. In total, 122 cellular proteins were identified by LC-MS/MS. Gene Ontology and KEGG databases were used to map these proteins to different cellular processes, locations and functions. Interestingly, 10.24% of identified cellular proteins were involved in the process of translation. Follow up experiments demonstrated that expression of GP5 in transfected cells led to inhibition of translation of reporter genes. Interaction between GP5 and ATP synthase subunit alpha (ATP5A) was further confirmed by co-immunoprecipitation suggesting a possible role of GP5 in regulation of ATP production in cells. These data contribute to a better understanding of GP5’s role in viral pathogenesis and virus-host interactions.
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Metadaten
Titel
Characterization of the interactome of the porcine reproductive and respiratory syndrome virus glycoprotein-5
verfasst von
Maodong Zhang
Alexander Zakhartchouk
Publikationsdatum
01.03.2018
Verlag
Springer Vienna
Erschienen in
Archives of Virology / Ausgabe 6/2018
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-018-3787-9

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