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Clinical Oral Investigations

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01.12.2012 | Original Article

Quantitative analysis of microbiota in saliva, supragingival, and subgingival plaque of Chinese adults with chronic periodontitis

verfasst von: Jiayan He, Wujing Huang, Zhiwen Pan, Honghua Cui, Ganggang Qi, Xueping Zhou, Hui Chen

Erschienen in: Clinical Oral Investigations | Ausgabe 6/2012

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Abstract

Objective

The aim of this study was to determine the profiles of periodontopathogenic bacteria in a Chinese population using quantitative real-time polymerase chain reaction (qRT-PCR).

Materials and methods

Twenty-four periodontally healthy Chinese subjects and 60 patients with chronic periodontitis (CP) were enrolled in this cross-sectional study. qRT-PCR was used to quantify Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum, Porphyromonas gingivalis, and Prevotella intermedia as well as total bacterial counts from 252 samples collected from the saliva, supragingival plaque, and subgingival plaque of all 84 subjects.

Results

The detection frequency of A. actinomycetemcomitans was less than 50%. F. nucleatum was detected in all subjects and CP patients had higher bacterial loads than healthy subjects. The median proportion of F. nucleatum was significantly higher in subgingival plaque than in supragingival plaque and saliva. P. gingivalis and P. intermedia had higher detection frequencies and bacterial loads in CP patients than in healthy subjects. The median proportion of P. gingivalis was significantly different among the three intraoral locations in the CP group and its proportion in subgingival plaque was 9.01%. Moreover, strong positive Spearman’s correlations were found in A. actinomycetemcomitans, P. gingivalis, and P. intermedia counts across the three intraoral locations.

Conclusion

The presence and bacteria loads of these four bacteria in this Chinese population are similar to those from other populations.

Clinical relevance

Examination of bacterial detection frequency and loads in Chinese adults may assist microbial studies of periodontal disease and will shed light on periodontal disease diagnosis and treatment using antibiotics in the Chinese population.

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Titel: Quantitative analysis of microbiota in saliva, supragingival, and subgingival plaque of Chinese adults with chronic periodontitis
verfasst von: Jiayan He
Wujing Huang
Zhiwen Pan
Honghua Cui
Ganggang Qi
Xueping Zhou
Hui Chen
Publikationsdatum: 01.12.2012
Verlag: Springer-Verlag
Erschienen in: Clinical Oral Investigations / Ausgabe 6/2012
Print ISSN: 1432-6981
Elektronische ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-011-0654-4

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Quantitative analysis of microbiota in saliva, supragingival, and subgingival plaque of Chinese adults with chronic periodontitis

Abstract

Objective

Materials and methods

Results

Conclusion

Clinical relevance

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Abstract

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Materials and methods

Results

Conclusion

Clinical relevance

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