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Clinical Oral Investigations

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21.05.2019 | Original Article

Investigating unset endodontic sealers’ eugenol and hydrocortisone roles in modulating the initial steps of inflammation

verfasst von: Charlotte Jeanneau, Thomas Giraud, Jean-Louis Milan, Imad About

Erschienen in: Clinical Oral Investigations | Ausgabe 2/2020

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Abstract

Introduction

Endodontic treatment success is achieved not only when the cement provides a hermetic seal but also when the injured periapical tissue is regenerated. However, an exaggerated inflammatory reaction hinders tissue regeneration and it has been shown that dental materials affect the inflammatory response through modulation of cytokine secretion. This work was set to investigate the effects of the presence of hydrocortisone in zinc oxide eugenol sealers (Endomethasone N) on modulating the initial steps of inflammation in vitro.

Material and methods

Hydrocortisone and eugenol leaching from Endomethasone N and Pulp Canal Sealer (PCS) were quantified by ELISA and spectrofluorometry, respectively. The effects of Endomethasone N and Pulp Canal Sealer were studied on lipopolysaccharides (LPS)-stimulated human periodontal ligament (hPDL) cells. Cytokine (IL-6, TNF-α) secretion from cells was quantified by ELISA. Inflammatory cell (THP-1) adhesion to activated endothelial cells, their migration and activation were studied in vitro.

Results

Endomethasone N decreased secretion of IL-6 and TNF-α from hPDL cells. THP-1 adhesion to activated endothelial cells (HUVECs) and migration significantly decreased with Endomethasone N while no effect was observed with PCS. Activation of THP-1 decreased with both materials’ extracts but was significantly lower with Endomethasone N than with PCS.

Conclusion

These results performed in vitro show that Endomethasone N anti-inflammatory effects are due to the presence of hydrocortisone.

Clinical relevance

Endomethasone N has potential local anti-inflammatory effects which appear to be due to its hydrocortisone rather than eugenol content. Decreasing the inflammatory response is a pre-requisite to initiate the periapical healing.

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Titel: Investigating unset endodontic sealers’ eugenol and hydrocortisone roles in modulating the initial steps of inflammation
verfasst von: Charlotte Jeanneau
Thomas Giraud
Jean-Louis Milan
Imad About
Publikationsdatum: 21.05.2019
Verlag: Springer Berlin Heidelberg
Erschienen in: Clinical Oral Investigations / Ausgabe 2/2020
Print ISSN: 1432-6981
Elektronische ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-019-02957-2

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Springer Medizin

Investigating unset endodontic sealers’ eugenol and hydrocortisone roles in modulating the initial steps of inflammation

Abstract

Introduction

Material and methods

Results

Conclusion

Clinical relevance

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Springer Medizin

Abstract

Introduction

Material and methods

Results

Conclusion

Clinical relevance

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