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IDH1/2 mutation detection in gliomas

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Abstract

Somatic mutations of isocitrate dehydrogenase 1 and 2 (IDH1/2) are strongly associated with pathological subtypes, genetic profiles, and clinical features in gliomas. The IDH1/2 status is currently regarded as one of the most important molecular markers in gliomas and should be assessed accurately and robustly. However, the methods used for IDH1/2 testing are not fully standardized. The purpose of this paper is to review the clinical significance of IDH1/2 mutations and the methods used for IDH1/2 testing. The optimal method for IDH1/2 testing varies depending on a number of factors, including the purpose, sample types, sample number, or laboratory equipment. It is therefore important to acknowledge the advantages and disadvantages of each method.

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Acknowledgments

This work was supported by JSPS KAKENHI Grant Numbers 26861171 (H.A.), 25462283 (K.I.) and by the National Cancer Center Research and Development Fund 23-A-50 (K.I.).

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Authors and Affiliations

  1. Department of Neurosurgery and Neuro-Oncology, National Cancer Center Hospital, Tokyo, Japan

    Hideyuki Arita & Yoshitaka Narita

  2. Department of Neurosurgery, Osaka University Graduate School of Medicine, 2-2, Yamadaoka, Suita, Osaka, 565-0871, Japan

    Hideyuki Arita, Naoya Hashimoto & Toshiki Yoshimine

  3. Department of Pathology and Clinical Laboratories, National Cancer Center Hospital, Tokyo, Japan

    Akihiko Yoshida

  4. Division of Brain Tumor Translational Research, National Cancer Center Research Institute, Tokyo, Japan

    Koichi Ichimura

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  1. Hideyuki Arita
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Arita, H., Narita, Y., Yoshida, A. et al. IDH1/2 mutation detection in gliomas. Brain Tumor Pathol 32, 79–89 (2015). https://doi.org/10.1007/s10014-014-0197-x

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