Elsevier

Molecules and Cells

Volume 30, Issue 6, December 2010, Pages 563-568
Molecules and Cells

Chemoattractant-Mediated Rap1 Activation Requires GPCR/G Proteins

https://doi.org/10.1007/s10059-010-0153-5Get rights and content
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Rap1 is rapidly activated upon chemoattractant stimulation and plays an important role in cell adhesion and cy- toskeletal reorganization during chemotaxis. Here, we demonstrate that G-protein coupled receptors and G-proteins are essential for chemoattractant-mediated Rap1 activation in Dictyostelium. The rapid Rap1 activation upon cAMP chemoattractant stimulation was absent in cells lacking chemoattractant cAMP receptors cAR1/cAR3 or a subunit of the heterotrimeric G-protein complex Gα2. Loss of guanylyl cyclases GCA/SGC or a cGMP-binding protein GbpC exhibited no effect on Rap1 activation kinetics. These results suggest that Rap1, a key regulator for the regulation of cytoskeletal reorganization during cell movement, is activated through the G-protein coupled receptors cAR1/cAR3 and Gα2 proteins in a way independent of the cGMP signaling pathway.

Keywords:

chemotaxis
Dictyostelium
GPCR
Rap1
signal transduction

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