Abstract
5-Hydroxytryptamine (5-HT, serotonin) plays protective or detrimental roles in the development of ischemic damage. In the present study, we investigated the time-course changes in 5-HT1A receptor protein expression in the gerbil hippocampal CA1 region after transient global cerebral ischemia. 5-HT1A receptor immunoreactivity in the stratum pyramidale (SP) of the CA1 region was decreased from 6 h and hardly observed 1 and 2 days after ischemic insult. Five and 10 days after ischemia/reperfusion, 5-HT1A receptor immunoreactivity was increased and detected in astrocyte of the ischemic CA1 region. Furthermore, the pattern of changes in 5-HT1A receptor protein levels in the CA1 region after ischemia–reperfusion was similar to that observed in the immunohistochemical data. These results indicate that 5-HT1A receptor protein expressions may be related with the ischemia-induced neuronal death and the function of astrocytes in the ischemic hippocampal CA1 region.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Kirino T (1982) Delayed neuronal death in the gerbil hippocampus following ischemia. Brain Res 239(1):57–69
Ajami M, Eghtesadi S, Razaz JM, Kalantari N, Habibey R, Nilforoushzadeh MA, Zarrindast M, Pazoki-Toroudi H (2011) Expression of Bcl-2 and Bax after hippocampal ischemia in DHA + EPA treated rats. Neurol Sci 32(5):811–818. doi:10.1007/s10072-011-0621-5
He J, Huang C, Jiang J, Lv L (2013) Propofol exerts hippocampal neuron protective effects via up-regulation of metallothionein-3. Neurol Sci 34(2):165–171. doi:10.1007/s10072-012-0978-0
Benveniste H, Drejer J, Schousboe A, Diemer NH (1984) Elevation of the extracellular concentrations of glutamate and aspartate in rat hippocampus during transient cerebral ischemia monitored by intracerebral microdialysis. J Neurochem 43(5):1369–1374
Schmitz D, Empson RM, Heinemann U (1995) Serotonin and 8-OH-DPAT reduce excitatory transmission in rat hippocampal area CA1 via reduction in presumed presynaptic Ca2+ entry. Brain Res 701(1–2):249–254
Globus MY, Wester P, Busto R, Dietrich WD (1992) Ischemia-induced extracellular release of serotonin plays a role in CA1 neuronal cell death in rats. Stroke 23(11):1595–1601
do Kim H, Li H, Yoo KY, Lee BH, Hwang IK, Won MH (2007) Effects of fluoxetine on ischemic cells and expressions in BDNF and some antioxidants in the gerbil hippocampal CA1 region induced by transient ischemia. Exp Neurol 204(2):748–758. doi:10.1016/j.expneurol.2007.01.008
Lee CH, Park JH, Yoo KY, Choi JH, Hwang IK, Ryu PD, Kim DH, Kwon YG, Kim YM et al (2011) Pre- and post-treatments with escitalopram protect against experimental ischemic neuronal damage via regulation of BDNF expression and oxidative stress. Exp Neurol 229(2):450–459. doi:10.1016/j.expneurol.2011.03.015
Grasby PM, Sharp T, Allen T, Kelly PA, Grahame-Smith DG (1992) Effects of the 5-HT1A partial agonists gepirone, ipsapirone and buspirone on local cerebral glucose utilization in the conscious rat. Psychopharmacology 106(1):97–101
Marco I, Valhondo M, Martin-Fontecha M, Vazquez-Villa H, Del Rio J, Planas A, Sagredo O, Ramos JA, Torrecillas IR et al (2011) New serotonin 5-HT(1A) receptor agonists with neuroprotective effect against ischemic cell damage. J Med Chem 54(23):7986–7999. doi:10.1021/jm2007886
Azmitia EC, Gannon PJ, Kheck NM, Whitaker-Azmitia PM (1996) Cellular localization of the 5-HT1A receptor in primate brain neurons and glial cells. Neuropsychopharmacol 14(1):35–46. doi:10.1016/S0893-133X(96)80057-1
Pazos A, Palacios JM (1985) Quantitative autoradiographic mapping of serotonin receptors in the rat brain. I. Serotonin-1 receptors. Brain Res 346(2):205–230
Kozuka M, Iwata N (1995) Changes in levels of monoamines and their metabolites in incompletely ischemic brains of spontaneously hypertensive rats. Neurochem Res 20(12):1429–1435
An SJ, Kim DS (2011) Alterations in serotonin receptors and transporter immunoreactivities in the hippocampus in the rat unilateral hypoxic-induced epilepsy model. Cell Mol Neurobiol 31(8):1245–1255. doi:10.1007/s10571-011-9726-x
Matsuyama S, Nei K, Tanaka C (1996) Regulation of glutamate release via NMDA and 5-HT1A receptors in guinea pig dentate gyrus. Brain Res 728(2):175–180
Wang Z, Du Q, Wang F, Liu Z, Li B, Wang A, Wang Y (2004) Microarray analysis of gene expression on herbal glycoside recipes improving deficient ability of spatial learning memory in ischemic mice. J Neurochem 88(6):1406–1415
Ouyang YB, Voloboueva LA, Xu LJ, Giffard RG (2007) Selective dysfunction of hippocampal CA1 astrocytes contributes to delayed neuronal damage after transient forebrain ischemia. J Neurosci 27(16):4253–4260. doi:10.1523/JNEUROSCI.0211-07.2007
Rossi DJ, Brady JD, Mohr C (2007) Astrocyte metabolism and signaling during brain ischemia. Nat Neurosci 10(11):1377–1386. doi:10.1038/nn2004
Shen LH, Li Y, Chopp M (2010) Astrocytic endogenous glial cell derived neurotrophic factor production is enhanced by bone marrow stromal cell transplantation in the ischemic boundary zone after stroke in adult rats. Glia 58(9):1074–1081. doi:10.1002/glia.20988
Hirth IC, Deitmer JW (2006) 5-Hydroxytryptamine-mediated increase in glutamate uptake by the leech giant glial cell. Glia 54(8):786–794. doi:10.1002/glia.20417
Acknowledgments
This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (NRF-2012R1A1A1007298).
Conflict of interest
The authors declare that they have no conflict of interest.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Lee, C.H., Ahn, J.H. & Won, MH. New expression of 5-HT1A receptor in astrocytes in the gerbil hippocampal CA1 region following transient global cerebral ischemia. Neurol Sci 36, 383–389 (2015). https://doi.org/10.1007/s10072-014-1958-3
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10072-014-1958-3