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Erschienen in: European Journal of Clinical Microbiology & Infectious Diseases 2/2008

01.02.2008 | Article

Detection of hepatitis C virus antibodies in oral fluid specimens for prevalence studies

verfasst von: V. González, E. Martró, C. Folch, A. Esteve, L. Matas, A. Montoliu, J. R. Grífols, F. Bolao, C. Tural, R. Muga, J. V. Parry, V. Ausina, J. Casabona

Erschienen in: European Journal of Clinical Microbiology & Infectious Diseases | Ausgabe 2/2008

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Abstract

Within the framework of hepatitis C virus (HCV) prevalence monitoring, we evaluated oral fluid (OF), which is richer in IgG than whole saliva, as a possible alternative to serum for the detection of HCV antibodies. Paired OF and serum samples were collected from 90 individuals, including 45 HCV-positives and 45 HCV-negatives. The detection of HCV antibodies in both serum and OF was performed using the Ortho HCV 3.0 SAVe enzyme-linked immunosorbent assay (ELISA) (Ortho-Clinical Diagnostics, Inc., Raritan, NJ), but a modified, more sensitive protocol was used to process OF. The sensitivity and specificity of this assay were 86.67% (95% confidence interval (CI): 72.51–94.46%) and 100% (95% CI: 90.20–99.80%) in OF and 100% in serum. The correlation obtained between both types of clinical specimens was excellent (k: 0.87, 95% CI: 0.66–1.07). However, the negative predictive value (NPV) of the assay in OF decreased with the prevalence of HCV infection in the population studied. Our results suggest that the modified Ortho HCV 3.0 SAVe ELISA is suitable for the detection of HCV antibodies in OF for epidemiological studies. Using this assay, we observed an unadjusted anti-HCV prevalence of 78.6% among a population of intravenous drug users; when adjusted to account for assay sensitivity, this prevalence may be closer to 90%.
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Metadaten
Titel
Detection of hepatitis C virus antibodies in oral fluid specimens for prevalence studies
verfasst von
V. González
E. Martró
C. Folch
A. Esteve
L. Matas
A. Montoliu
J. R. Grífols
F. Bolao
C. Tural
R. Muga
J. V. Parry
V. Ausina
J. Casabona
Publikationsdatum
01.02.2008
Verlag
Springer-Verlag
Erschienen in
European Journal of Clinical Microbiology & Infectious Diseases / Ausgabe 2/2008
Print ISSN: 0934-9723
Elektronische ISSN: 1435-4373
DOI
https://doi.org/10.1007/s10096-007-0408-z

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