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European Journal of Clinical Microbiology & Infectious Diseases

Erschienen in:

01.08.2012 | Review

Next-generation and whole-genome sequencing in the diagnostic clinical microbiology laboratory

verfasst von: W. M. Dunne Jr, L. F. Westblade, B. Ford

Erschienen in: European Journal of Clinical Microbiology & Infectious Diseases | Ausgabe 8/2012

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Abstract

The identification and/or prediction of the antimicrobial resistance of microorganisms in clinical materials solely by molecular means in the diagnostic microbiology laboratory is not novel. However, the ability to sequence multitudes of bacterial genomes and deliver and interpret the resultant sequence information in near “real-time” is the basis of next-generation sequencing (NGS) technologies. There have been numerous applications and successes of NGS applications in the clinical and public health domain. However, none have, as yet, delivered perhaps the most sought after application, i.e., the generation of microbial sequence data for “real-time” patient management. In this review, we discuss the use of NGS and whole-genome sequencing (WGS) of microorganisms as a logical next step for the routine diagnosis of infection and the prediction of antimicrobial susceptibility in the clinical microbiology laboratory.

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Titel: Next-generation and whole-genome sequencing in the diagnostic clinical microbiology laboratory
verfasst von: W. M. Dunne Jr
L. F. Westblade
B. Ford
Publikationsdatum: 01.08.2012
Verlag: Springer-Verlag
Erschienen in: European Journal of Clinical Microbiology & Infectious Diseases / Ausgabe 8/2012
Print ISSN: 0934-9723
Elektronische ISSN: 1435-4373
DOI: https://doi.org/10.1007/s10096-012-1641-7

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