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Lasers in Medical Science
Erschienen in: Lasers in Medical Science 1/2009

01.01.2009 | Original article

Photodynamic antimicrobial chemotherapy (PACT) with methylene blue increases membrane permeability in Candida albicans

verfasst von: Ligia Maria Giroldo, Monalisa Poliana Felipe, Marco Antonio de Oliveira, Egberto Munin, Leandro Procópio Alves, Maricilia Silva Costa

Erschienen in: Lasers in Medical Science | Ausgabe 1/2009

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Abstract

Photodynamic antimicrobial chemotherapy (PACT) is a potential antimicrobial therapy that combines light and a photosensitizing drug, promoting a phototoxic effect on the treated cells, in general via oxidative damage. In this work we studied the effect of PACT, using methylene blue (MB), on the permeability of Candida albicans membrane. Our results demonstrated that the combination of MB and laser (684 nm) promoted a decrease in Candida growth. The inhibition was more pronounced in the presence of 0.05 mg/ml MB and with an energy density of 28 J/cm2. The decrease in Candida growth was associated with an increase in membrane permeabilization. Thus, we suggest that a PACT mechanism using MB can be related to damage in the plasma membranes of the cells.
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Metadaten
Titel
Photodynamic antimicrobial chemotherapy (PACT) with methylene blue increases membrane permeability in Candida albicans
verfasst von
Ligia Maria Giroldo
Monalisa Poliana Felipe
Marco Antonio de Oliveira
Egberto Munin
Leandro Procópio Alves
Maricilia Silva Costa
Publikationsdatum
01.01.2009
Verlag
Springer-Verlag
Erschienen in
Lasers in Medical Science / Ausgabe 1/2009
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI
https://doi.org/10.1007/s10103-007-0530-2

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