Skip to main content
Hauptrubriken
CME Facharzt-Training Webinare Zeitschriften Bücher e.Medpedia Podcast
Service
Jobbörse Info & Hilfe
Fachgebiete
Anästhesiologie Allgemeinmedizin Arbeitsmedizin Augenheilkunde Chirurgie Dermatologie Gynäkologie und Geburtshilfe HNO Innere Medizin Kardiologie Neurologie Onkologie und Hämatologie Orthopädie und Unfallchirurgie Pädiatrie Pathologie Psychiatrie Radiologie Rechtsmedizin Urologie Zahnmedizin
Themen
Klimawandel und Gesundheit Neues aus dem Markt COVID-19 Praxis und Beruf Seltene Erkrankungen GOÄ & EBM Panorama
Sammlungen
Kasuistiken Algorithmen & Infografiken Blickdiagnosen Arthropedia FlapFinder (für Dermatochirurgen) Videos Kongressberichterstattung Medizin für Apothekerinnen und Apotheker Für Ärztinnen und Ärzte in Weiterbildung Für Medizinstudierende

Live-Webinar: Aktuelle Leitlinien bei Herz-Kreislauf-Erkrankungen

Konkret geht es um den Diabetes-Patienten mit kardiovaskulären Erkrankungen oder Risiken, die Herzinsuffizienz sowie die kardiovaskuläre Primär- und Sekundärprävention. Sind Sie hier schon auf dem neuesten Stand? Unsere Experten beleuchten, wo und warum das bisherige Procedere geändert werden soll und was in der Praxis sinnvoll ist. Holen Sie sich Ihr Update und 2 CME-Punkte beim MMW-Webinar am 24. April von 17.30 bis 19 Uhr. 
Erweiterte Suche
Anmelden
nach oben
Lasers in Medical Science
Erschienen in: Lasers in Medical Science 1/2011

01.01.2011 | Review Article

Effects of low-power laser irradiation (LPLI) at different wavelengths and doses on oxidative stress and fibrogenesis parameters in an animal model of wound healing

verfasst von: Paulo Cesar Lock Silveira, Luciano A. Silva, Tiago P. Freitas, Alexandra Latini, Ricardo A. Pinho

Erschienen in: Lasers in Medical Science | Ausgabe 1/2011

Einloggen, um Zugang zu erhalten

Abstract

Gallium-arsenide (GaAs) and helium-neon (HeNe) lasers are the most commonly used low-energy lasers in physiotherapy for promoting wound healing and pain modulation. The aim of this study was investigate the effect of low-power laser irradiation (LPLI) at different wavelengths and doses on oxidative stress and fibrogenesis parameters in an animal model of wound healing. The animals were randomly divided into five groups (n = 6): Controls (skin injured animals without local or systemic treatment), skin injury treated with HeNe 1 J/cm2 (two seg); skin injury treated with HeNe 3 J/cm2 (six seg); skin injury treated with GaAs 1 J/cm2 (three seg); skin injury treated with GaAs 3 J/cm2 (nine seg). A single circular wound measuring 8 mm in diameter was surgically created on the back of the animal. The rats were irradiated at 2, 12, 24, 48, 72, 96, and 120 h after skin injury. The parameters, namely hydroxyproline content, activities of the antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT), and lipid (TBARS) and protein oxidation (carbonyl groups) measurements were assessed. In addition, wound size regression was also analyzed. The results showed an improvement in the wound healing reflected by the reduction in wound size and increased collagen synthesis. Moreover, a significant reduction in TBARS levels, carbonyl content, and SOD and CAT activities were observed after laser irradiation, particularly with the treatments HeNe laser 1 and 3 J/cm2 dose and GaAs 3 J/cm2 dose. The data strongly indicate that LPLI therapy is efficient in accelerating the skin wound healing process after wounding, probably by reducing the inflammatory phase and inducing collagen synthesis.
Literatur
1.
Miyata H, Genma T, Ohshima M et al (2006) Mitogen-activated protein kinase/extracellular signal-regulated protein kinase activation of cultured human dental pulp cells by low-power gallium-aluminium-arsenic laser irradiation. Int Endod J 39:238–244CrossRefPubMed
2.
Thawer HA, Houghton PE (1999) Effect of laser irradiation on the growth and development of fetal mouse limbs in an in vitro model. Lasers Surg Med 24:285–295CrossRefPubMed
3.
Conlan MJ, Rapley JW, Cobb CM (1996) Biostimulation of wound healing by low-energy laser irradiation. A review. J Clin Periodontol 23:492–496CrossRefPubMed
4.
Nascimento PM, Pinheiro AL, Salgado MA, Ramalho LM (2004) A preliminary report on the effect of laser therapy on the healing of cutaneous surgical wounds as a consequence of an inversely proportional relationship between wavelength and intensity: histological study in rats. Photomed Laser Surg 22:513–518CrossRefPubMed
5.
Pessoa ES, Melhado RM, Theodoro LH, Garcia VG (2004) A histologic assessment of the influence of low-intensity laser therapy on wound healing in steroid-treated animals. Photomed Laser Surg 22:199–204CrossRefPubMed
6.
Pinheiro AL, Meireles GC, Barros-Vieira AL, Almeida D, Carvalho CM, Santos JN (2004) Phototherapy improves healing of cutaneous wounds in nourished and undernourished Wistar rats. Braz Dent J 15:21–28
7.
Silveira PCL, Streck EL, Pinho RA (2007) Evaluation of mitochondrial respiratory chain activity in wound healing by low-level laser therapy. Photochem Photobio 86:279–282CrossRef
8.
Karu T, Pyatibrat L, Kalendo G (1995) Irradiation with He-Ne laser increases ATP level in cells cultivated in vitro. J Photochem Photobiol B: Biol 27:219–223CrossRef
9.
Silveira PCL, Silva LA, Fraga DB et al (2009) Evaluation of mitochondrial respiratory chain activity in muscle healing by low-level laser therapy. Photochem Photobiol 95:89–92CrossRef
10.
Serarslan G, Altug E, Kontas T, Atik E, Avci G (2007) Caffeic acid phenethyl ester accelerates cutaneous wound healing in a rat model and decreases oxidative stress. Clin Exp Dermatol 32:709–715CrossRefPubMed
11.
Phan T, Wang L, See P (2001) Phenolic compounds of Chromolaena odorata protect cultured skin cells from oxidative damage: implication for cutaneous wound healing. Biol Pharm Bull 24:1373–1379CrossRefPubMed
12.
Juránek I, Bezek S (2005) Controversy of free radical hypothesis: reactive oxygen species—cause or consequence of tissue injury? Gen Physiol Biophys 24:263–278PubMed
13.
Carvalho PTC, Mazzer N, Reis FA (2006) Analysis of the influence of low-power HeNe laser on the healing of skin wounds in diabetic and non-diabetic rats. Acta Cir Brás 21:177–183PubMed
14.
Morrone ET (1998) Muscular trauma treated with a Ga–Al–As diode laser: in vivo experimental study. Lasers Med Sci 13:293–298CrossRef
15.
Woessner JF (1961) The determination of hydroxyproline in tissue and protein samples containing small proportions of this amino acid. Arch Biochem Biophys 93:440–447CrossRefPubMed
16.
Draper HH, Hadley M (1990) Malondialdehyde determination as index of lipid peroxidation. Meth Enzymol 186:421–431CrossRefPubMed
17.
Levine RL, Garland D, Oliver CN (1990) Determination of carbonyl content in oxidatively modified proteins. Meth Enzymol 186:464–478CrossRefPubMed
18.
Bannister JV, Calabrese L (1987) Assays for SOD. Meth Biochem Anal 32:279–312CrossRef
19.
20.
Lowry OH, Rosebough NG, Farr AL (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265–275PubMed
21.
Li G, Feng X, Wang S (2005) Effects of Cu/Zn superoxide dismutase on strain injury-induced oxidative damage to skeletal muscle in rats. Physiol Res 54:193–1999PubMed
22.
Pereira LM, Hatanaka E, Martins EF et al (2008) Effect of oleic and linoleic acids on the inflammatory phase of wound healing in rats. Cell Biochem Funct 26:197–204CrossRefPubMed
23.
Henry G, Warren L, Garner MD (2003) Inflammatory mediators in wound healing. Surg Clin N Am 83:483–507CrossRefPubMed
24.
Pattwell DM, Jackson MJ (2004) Contraction-induced oxidants as mediators of adaptation and damage in skeletal muscle. Exerc Sport Sci Rev 32:14–18CrossRefPubMed
25.
Sommer AP, Pinheiro ALB, Mester AR et al (2001) Biostimulatory windows in low intensity laser activation: lasers, scanners and NASA’s light-emitting diode array system. J Clin Laser Med Surg 19:29–34CrossRefPubMed
26.
Karu T (1999) Primary and secondary mechanisms of action of visible to near-IR radiation on cells. J Photochem Photobiol B: Biol 49:1–17CrossRef
27.
Vekshin NL, Mironov GP (1982) Flavin-dependent oxygen uptake in mitochondria under illumination. Biofizika 27:537–539PubMed
28.
Passarella S, Casamassima F, Molinari S et al (1984) Increase of proton electrochemical potential and ATP synthesis in rat liver mitochondria irradiated in vitro by He-Ne laser. FEBS Lett 175:95–99CrossRefPubMed
29.
Karu T (1987) Photobiological fundamentals of low-power laser therapy. IEEE J Quantum Electron 23:1703–1717CrossRef
30.
Beauvoit B, Kitai T, Chance B (1994) Contribution of the mitochondrial compartment to the optical properties of the rat liver: a theoretical and practical approach. Biophys J 67:2501–2510CrossRefPubMed
31.
Cooper CE, Springett R (1997) Measurement of cytochrome oxidase and mitochondrial energetics by near-infrared spectroscopy. Philos Trans R Soc Lond B Biol Sci 352:669–676CrossRefPubMed
32.
Byrnes KR, Barna L, Chenault VM et al (2004) Photobiomodulation improves cutaneous wound healing in an animal model of type II diabetes. Photomed Laser Surg 22:281–290CrossRefPubMed
33.
34.
Fukushima K, Badlani N, Usas A et al (2001) The use of antifibrosis agent to improve muscle recovery after laceration. Am J Sports Med 29:394PubMed
35.
Reddy GK, Stehno-Bittel L, Enwemeka CS (1998) Laser photostimulation of collagen production in healing rabbit Achilles tendons. Lasers Surg Med 22:281–287CrossRefPubMed
36.
Stadler I, Lanzafame RJ, Evans R et al (2001) 830-nm irradiation increases the wound tensile strength in a diabetic murine model. Lasers Surg Med 28:220–226CrossRefPubMed
37.
Al-Watban F, Andres B (2003) Polychromatic LED therapy in burn healing of non-diabetic and diabetic rats. J Clin Laser Med Surg 21:249–258CrossRefPubMed
38.
Gillitzer R, Goebeler M (2001) Chemokines in cutaneous wound healing. J Leukoc Biol 69:513–521PubMed
39.
Ruszczak Z, Schwartz R (2000) Modern aspects of wound healing: an update. Dermatol Surg 26:219–229CrossRefPubMed
40.
Kerkweg U, Petrat F, Korth HG (2007) Disruption of skeletal myocytes initiates superoxide release: contribution of NAD(P)H oxidase. Shock 27:552–558CrossRefPubMed
41.
Albertini R, Villaverde AB, Aimbire F (2007) Anti-inflammatory effects of low-level laser therapy (LLLT) with two different red wavelengths (660 nm and 684 nm) in carrageenan-induced rat paw edema. J Photochem Photobiol B: Biol 89:50–55CrossRef
42.
Liu X, Zhou Y, Liu TC (2009) Effects of low-level laser irradiation on rat skeletal muscle injury after eccentric exercise. Photomed Laser Surg 27:863–869CrossRefPubMed
43.
Halliwell B, Gutteridge JMC (2007) Free Radical in Biology Medicine. University Press, Oxford, NY
44.
Musalmah M, Fairuz AH, Gapor MT et al (2002) Effect of vitamin E on plasma malondialdehyde, antioxidant enzyme levels and the rates of wound closures during wound healing in normal and diabetic rats. Asia Pacific J Clin Nurt 11:448–451CrossRef
45.
Shukla A, Rasik AM, Patnaik GK (1997) Depletion of reduced glutathione, ascorbic acid, vitamin E and antioxidant defence enzymes in a healing cutaneous wound. Free Radic Res 26:93–101CrossRefPubMed
Metadaten
Titel
Effects of low-power laser irradiation (LPLI) at different wavelengths and doses on oxidative stress and fibrogenesis parameters in an animal model of wound healing
verfasst von
Paulo Cesar Lock Silveira
Luciano A. Silva
Tiago P. Freitas
Alexandra Latini
Ricardo A. Pinho
Publikationsdatum
01.01.2011
Verlag
Springer-Verlag
Erschienen in
Lasers in Medical Science / Ausgabe 1/2011
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI
https://doi.org/10.1007/s10103-010-0839-0

Weitere Artikel der Ausgabe 1/2011

Lasers in Medical Science 1/2011 Zur Ausgabe

Original Article

Low-level laser therapy (LLLT; 780 nm) acts differently on mRNA expression of anti- and pro-inflammatory mediators in an experimental model of collagenase-induced tendinitis in rat

Original Article

Effects of laser and acid etching and air abrasion on mineral content of dentin

Original Article

Effect and mechanism of 5-aminolevulinic acid-mediated photodynamic therapy in esophageal cancer

Original Article

Diode laser treatment in therapy-resistant allergic rhinitis: impact on nasal obstruction and associated symptoms

Original Article

Effect of sealants on laser fluorescence caries detection in primary teeth

Original Article

An in-vitro investigation of skin tissue soldering using gold nanoshells and diode laser