Skip to main content
Erschienen in: Lasers in Medical Science 5/2012

01.09.2012 | Original Article

GaAs 904-nm laser irradiation improves myofiber mass recovery during regeneration of skeletal muscle previously damaged by crotoxin

verfasst von: Lucila H. Silva, Meiricris T. Silva, Rita M. Gutierrez, Talita C. Conte, Cláudio A. Toledo, Marcelo S. Aoki, Richard E. Liebano, Elen H. Miyabara

Erschienen in: Lasers in Medical Science | Ausgabe 5/2012

Einloggen, um Zugang zu erhalten

Abstract

This work investigated the effect of gallium arsenide (GaAs) irradiation (power: 5 mW; intensity: 77.14 mW/cm2, spot: 0.07 cm2) on regenerating skeletal muscles damaged by crotoxin (CTX). Male C57Bl6 mice were divided into six groups (n = 5 each): control, treated only with laser at doses of 1.5 J or 3 J, CTX-injured and, CTX-injured and treated with laser at doses of 1.5 J or 3 J. The injured groups received a CTX injection into the tibialis anterior (TA) muscle. After 3 days, TA muscles were submitted to GaAs irradiation at doses of 1.5 or 3 J (once a day, during 5 days) and were killed on the eighth day. Muscle histological sections were stained with hematoxylin and eosin (H&E) in order to determine the myofiber cross-sectional area (CSA), the previously injured muscle area (PIMA) and the area density of connective tissue. The gene expression of MyoD and myogenin was detected by real-time PCR. GaAs laser at a dose of 3 J, but not 1.5 J, significantly increased the CSA of regenerating myofibers and reduced the PIMA and the area density of intramuscular connective tissue of CTX-injured muscles. MyoD gene expression increased in the injured group treated with GaAs laser at a dose of 1.5 J. The CTX-injured, 3-J GaAs laser-treated, and the CTX-injured and treated with 3-J laser groups showed an increase in myogenin gene expression when compared to the control group. Our results suggest that GaAs laser treatment at a dose of 3 J improves skeletal muscle regeneration by accelerating the recovery of myofiber mass.
Literatur
1.
Zurück zum Zitat Hawke TJ, Garry DJ (2001) Myogenic satellite cells: physiology to molecular biology. J Appl Physiol 91:534–551PubMed Hawke TJ, Garry DJ (2001) Myogenic satellite cells: physiology to molecular biology. J Appl Physiol 91:534–551PubMed
2.
Zurück zum Zitat Ten Broek RW, Grefte S, Von den Hoff JW (2010) Regulatory factors and cell populations involved in skeletal muscle regeneration. J Cell Physiol 224:7–16PubMed Ten Broek RW, Grefte S, Von den Hoff JW (2010) Regulatory factors and cell populations involved in skeletal muscle regeneration. J Cell Physiol 224:7–16PubMed
3.
Zurück zum Zitat Mester E, Spiry T, Szende B, Tota JG (1971) Effect of laser rays on wound healing. Am J Surg 122:532–535PubMedCrossRef Mester E, Spiry T, Szende B, Tota JG (1971) Effect of laser rays on wound healing. Am J Surg 122:532–535PubMedCrossRef
4.
Zurück zum Zitat Mester E, Mester AF, Mester A (1985) The biomedical effects of laser application. Lasers Surg Med 5:31–39PubMedCrossRef Mester E, Mester AF, Mester A (1985) The biomedical effects of laser application. Lasers Surg Med 5:31–39PubMedCrossRef
5.
Zurück zum Zitat Yaakobi T, Maltz L, Oron U (1996) Promotion of bone repair in the cortical bone of the tibia in rats by low-energy laser (He-Ne) irradiation. Calcif Tissue Int 59:297–300PubMedCrossRef Yaakobi T, Maltz L, Oron U (1996) Promotion of bone repair in the cortical bone of the tibia in rats by low-energy laser (He-Ne) irradiation. Calcif Tissue Int 59:297–300PubMedCrossRef
6.
Zurück zum Zitat Luger EJ, Rochkind S, Wollman Y, Kogan G, Dekel S (1998) Effect of low-power laser irradiation on the mechanical properties of bone fracture healing in rats. Lasers Surg Med 22:97–102PubMedCrossRef Luger EJ, Rochkind S, Wollman Y, Kogan G, Dekel S (1998) Effect of low-power laser irradiation on the mechanical properties of bone fracture healing in rats. Lasers Surg Med 22:97–102PubMedCrossRef
7.
Zurück zum Zitat Rochkind S, Ouaknine GE (1992) New trend in neuroscience: low-power laser effect on peripheral and central nervous system (basic science, preclinical and clinical studies). Neurol Res 14:2–11PubMed Rochkind S, Ouaknine GE (1992) New trend in neuroscience: low-power laser effect on peripheral and central nervous system (basic science, preclinical and clinical studies). Neurol Res 14:2–11PubMed
8.
Zurück zum Zitat Van Breugel HH, Bar PR (1993) He-Ne laser irradiation affects proliferation of cultured rat Schwann cells in a dose-dependent manner. J Neurocytol 22:185–190PubMedCrossRef Van Breugel HH, Bar PR (1993) He-Ne laser irradiation affects proliferation of cultured rat Schwann cells in a dose-dependent manner. J Neurocytol 22:185–190PubMedCrossRef
9.
Zurück zum Zitat Bibikova A, Belkin V, Oron U (1994) Enhancement of angiogenesis in regenerating gastrocnemius muscle of the toad (Bufo viridis) by low-energy laser irradiation. Anat Embryol (Berl) 190:597–602CrossRef Bibikova A, Belkin V, Oron U (1994) Enhancement of angiogenesis in regenerating gastrocnemius muscle of the toad (Bufo viridis) by low-energy laser irradiation. Anat Embryol (Berl) 190:597–602CrossRef
10.
Zurück zum Zitat Bibikova A, Oron U (1995) Regeneration in denervated toad (Bufo viridis) gastrocnemius muscle and the promotion of the process by low-energy laser irradiation. Anat Rec 241:123–128PubMedCrossRef Bibikova A, Oron U (1995) Regeneration in denervated toad (Bufo viridis) gastrocnemius muscle and the promotion of the process by low-energy laser irradiation. Anat Rec 241:123–128PubMedCrossRef
11.
Zurück zum Zitat Oliveira NM, Parizzotto NA, Salvini TF (1999) GaAs (904-nm) laser radiation does not affect muscle regeneration in mouse skeletal muscle. Lasers Surg Med 25:13–21PubMedCrossRef Oliveira NM, Parizzotto NA, Salvini TF (1999) GaAs (904-nm) laser radiation does not affect muscle regeneration in mouse skeletal muscle. Lasers Surg Med 25:13–21PubMedCrossRef
12.
Zurück zum Zitat Servetto N, Cremonezzi D, Simes JC, Moya M, Soriano F, Palma JA, Campana VR (2010) Evaluation of inflammatory biomarkers associated with oxidative stress and histological assessment of low-level laser therapy in experimental myopathy. Lasers Surg Med 42:577–583PubMedCrossRef Servetto N, Cremonezzi D, Simes JC, Moya M, Soriano F, Palma JA, Campana VR (2010) Evaluation of inflammatory biomarkers associated with oxidative stress and histological assessment of low-level laser therapy in experimental myopathy. Lasers Surg Med 42:577–583PubMedCrossRef
13.
Zurück zum Zitat Leal Junior EC, Lopes-Martins RA, Frigo L, De Marchi T, Rossi RP, de Godoi V, Tomazoni SS, Silva DP, Basso M, Filho PL, de Valls Corsetti F, Iversen VV, Bjordal JM (2010) Effects of low-level laser therapy (LLLT) in the development of exercise-induced skeletal muscle fatigue and changes in biochemical markers related to postexercise recovery. J Orthop Sports Phys Ther 40:524–532PubMed Leal Junior EC, Lopes-Martins RA, Frigo L, De Marchi T, Rossi RP, de Godoi V, Tomazoni SS, Silva DP, Basso M, Filho PL, de Valls Corsetti F, Iversen VV, Bjordal JM (2010) Effects of low-level laser therapy (LLLT) in the development of exercise-induced skeletal muscle fatigue and changes in biochemical markers related to postexercise recovery. J Orthop Sports Phys Ther 40:524–532PubMed
14.
Zurück zum Zitat Karu T (1989) Laser biostimulation: a photobiological phenomenon. J Photochem Photobiol B 3:638–640PubMedCrossRef Karu T (1989) Laser biostimulation: a photobiological phenomenon. J Photochem Photobiol B 3:638–640PubMedCrossRef
15.
Zurück zum Zitat da Silva JP, da Silva MA, Almeida AP, Lombardi Junior I, Matos AP (2010) Laser therapy in the tissue repair process: a literature review. Photomed Laser Surg 28:17–21PubMedCrossRef da Silva JP, da Silva MA, Almeida AP, Lombardi Junior I, Matos AP (2010) Laser therapy in the tissue repair process: a literature review. Photomed Laser Surg 28:17–21PubMedCrossRef
16.
Zurück zum Zitat Basford JR (1993) Laser therapy: scientific basis and clinical role. Orthopedics 16:541–547PubMed Basford JR (1993) Laser therapy: scientific basis and clinical role. Orthopedics 16:541–547PubMed
17.
Zurück zum Zitat Basford JR (1995) Low intensity laser therapy: still not an established clinical tool. Lasers Surg Med 16:331–342PubMedCrossRef Basford JR (1995) Low intensity laser therapy: still not an established clinical tool. Lasers Surg Med 16:331–342PubMedCrossRef
18.
Zurück zum Zitat Nakano J, Kataoka H, Sakamoto J, Origuchi T, Okita M, Yoshimura T (2009) Low-level laser irradiation promotes the recovery of atrophied gastrocnemius skeletal muscle in rats. Exp Physiol 94:1005–1015PubMedCrossRef Nakano J, Kataoka H, Sakamoto J, Origuchi T, Okita M, Yoshimura T (2009) Low-level laser irradiation promotes the recovery of atrophied gastrocnemius skeletal muscle in rats. Exp Physiol 94:1005–1015PubMedCrossRef
19.
Zurück zum Zitat Hotta PT, Hotta TH, Bataglion C, Bataglion SA, de Souza Coronatto EA, Siessere S, Regalo SC (2010) Emg analysis after laser acupuncture in patients with temporomandibular dysfunction (TMD). Implications for practice. Complement Ther Clin Pract 16:158–160PubMedCrossRef Hotta PT, Hotta TH, Bataglion C, Bataglion SA, de Souza Coronatto EA, Siessere S, Regalo SC (2010) Emg analysis after laser acupuncture in patients with temporomandibular dysfunction (TMD). Implications for practice. Complement Ther Clin Pract 16:158–160PubMedCrossRef
20.
Zurück zum Zitat Weiss N, Oron U (1992) Enhancement of muscle regeneration in the rat gastrocnemius muscle by low energy laser irradiation. Anat Embryol (Berl) 186:497–503CrossRef Weiss N, Oron U (1992) Enhancement of muscle regeneration in the rat gastrocnemius muscle by low energy laser irradiation. Anat Embryol (Berl) 186:497–503CrossRef
21.
Zurück zum Zitat Bibikova A, Oron U (1993) Promotion of muscle regeneration in the toad (Bufo viridis) gastrocnemius muscle by low-energy laser irradiation. Anat Rec 235:374–380PubMedCrossRef Bibikova A, Oron U (1993) Promotion of muscle regeneration in the toad (Bufo viridis) gastrocnemius muscle by low-energy laser irradiation. Anat Rec 235:374–380PubMedCrossRef
22.
Zurück zum Zitat Ben-Dov N, Shefer G, Irintchev A, Wernig A, Oron U, Halevy O (1999) Low-energy laser irradiation affects satellite cell proliferation and differentiation in vitro. Biochim Biophys Acta 1448:372–380PubMedCrossRef Ben-Dov N, Shefer G, Irintchev A, Wernig A, Oron U, Halevy O (1999) Low-energy laser irradiation affects satellite cell proliferation and differentiation in vitro. Biochim Biophys Acta 1448:372–380PubMedCrossRef
23.
Zurück zum Zitat Dourado DM, Favero S, Baranauskas V, da Cruz-Hofling MA (2003) Effects of the Ga-As laser irradiation on myonecrosis caused by Bothrops Moojeni snake venom. Lasers Surg Med 33:352–357PubMedCrossRef Dourado DM, Favero S, Baranauskas V, da Cruz-Hofling MA (2003) Effects of the Ga-As laser irradiation on myonecrosis caused by Bothrops Moojeni snake venom. Lasers Surg Med 33:352–357PubMedCrossRef
24.
Zurück zum Zitat Mester E (1982) Biostimulating effect of laser beams. Z Exp Chir 15:67–74PubMed Mester E (1982) Biostimulating effect of laser beams. Z Exp Chir 15:67–74PubMed
25.
Zurück zum Zitat World Association of Laser Therapy (WALT) (2006) Consensus agreement on the design and conduct of clinical studies with low-level laser therapy and light therapy for musculoskeletal pain and disorders. Photomed Laser Surg 24:761–762 World Association of Laser Therapy (WALT) (2006) Consensus agreement on the design and conduct of clinical studies with low-level laser therapy and light therapy for musculoskeletal pain and disorders. Photomed Laser Surg 24:761–762
26.
Zurück zum Zitat Conte TC, Franco DV, Baptista IL, Bueno CR Jr, Selistre-de-Araujo HS, Brum PC, Moriscot AS, Miyabara EH (2008) Radicicol improves regeneration of skeletal muscle previously damaged by crotoxin in mice. Toxicon 52:146–155PubMedCrossRef Conte TC, Franco DV, Baptista IL, Bueno CR Jr, Selistre-de-Araujo HS, Brum PC, Moriscot AS, Miyabara EH (2008) Radicicol improves regeneration of skeletal muscle previously damaged by crotoxin in mice. Toxicon 52:146–155PubMedCrossRef
27.
Zurück zum Zitat Mathieu O, Cruz-Orive LM, Hoppeler H, Weibel ER (1981) Measuring error and sampling variation in stereology: comparison of the efficiency of various methods for planar image analysis. J Microsc 121:75–88PubMedCrossRef Mathieu O, Cruz-Orive LM, Hoppeler H, Weibel ER (1981) Measuring error and sampling variation in stereology: comparison of the efficiency of various methods for planar image analysis. J Microsc 121:75–88PubMedCrossRef
28.
Zurück zum Zitat Warrell DA (1996) Clinical features of envenoming by snake bites. In: Bon C, Goyffon M (eds) Envenomings and their treatments. Fondation Marcel Me´rieux, Lyon, pp 63–76 Warrell DA (1996) Clinical features of envenoming by snake bites. In: Bon C, Goyffon M (eds) Envenomings and their treatments. Fondation Marcel Me´rieux, Lyon, pp 63–76
29.
Zurück zum Zitat Gutierrez JM, Ownby CL (2003) Skeletal muscle degeneration induced by venom phospholipases A2: insights into the mechanisms of local and systemic myotoxicity. Toxicon 42:915–931PubMedCrossRef Gutierrez JM, Ownby CL (2003) Skeletal muscle degeneration induced by venom phospholipases A2: insights into the mechanisms of local and systemic myotoxicity. Toxicon 42:915–931PubMedCrossRef
30.
Zurück zum Zitat Duguez S, Bihan MC, Gouttefangeas D, Feasson L, Freyssenet D (2003) Myogenic and nonmyogenic cells differentially express proteinases, Hsc/Hsp70, and BAG-1 during skeletal muscle regeneration. Am J Physiol Endocrinol Metab 285:E206–E215PubMed Duguez S, Bihan MC, Gouttefangeas D, Feasson L, Freyssenet D (2003) Myogenic and nonmyogenic cells differentially express proteinases, Hsc/Hsp70, and BAG-1 during skeletal muscle regeneration. Am J Physiol Endocrinol Metab 285:E206–E215PubMed
31.
Zurück zum Zitat Fillipin LI, Mauriz JL, Vedovelli K, Moreira AJ, Zettler CG, Lech O, Marroni NP, Gonzalez-Gallego J (2005) Low-level laser therapy (LLLT) prevents oxidative stress and reduces fibrosis in rat traumatized Achilles tendon. Lasers Surg Med 37:293–300PubMedCrossRef Fillipin LI, Mauriz JL, Vedovelli K, Moreira AJ, Zettler CG, Lech O, Marroni NP, Gonzalez-Gallego J (2005) Low-level laser therapy (LLLT) prevents oxidative stress and reduces fibrosis in rat traumatized Achilles tendon. Lasers Surg Med 37:293–300PubMedCrossRef
32.
Zurück zum Zitat Shefer G, Barash I, Oron U, Halevy O (2003) Low-energy laser irradiation enhances de novo protein synthesis via its effects on translation-regulatory proteins in skeletal muscle myoblasts. Biochim Biophys Acta 1593:131–139PubMedCrossRef Shefer G, Barash I, Oron U, Halevy O (2003) Low-energy laser irradiation enhances de novo protein synthesis via its effects on translation-regulatory proteins in skeletal muscle myoblasts. Biochim Biophys Acta 1593:131–139PubMedCrossRef
33.
Zurück zum Zitat Leal Junior EC, Lopes-Martins RA, de Almeida P, Ramos L, Iversen VV, Bjordal JM (2010) Effect of low-level laser therapy (GaAs 904 nm) in skeletal muscle fatigue and biochemical markers of muscle damage in rats. Eur J Appl Physiol 108:1083–1088PubMedCrossRef Leal Junior EC, Lopes-Martins RA, de Almeida P, Ramos L, Iversen VV, Bjordal JM (2010) Effect of low-level laser therapy (GaAs 904 nm) in skeletal muscle fatigue and biochemical markers of muscle damage in rats. Eur J Appl Physiol 108:1083–1088PubMedCrossRef
34.
Zurück zum Zitat Shefer G, Partridge TA, Heslop L, Gross JG, Oron U, Halevy O (2002) Low-energy laser irradiation promotes the survival and cell cycle entry of skeletal muscle satellite cells. J Cell Sci 115:1461–1469PubMed Shefer G, Partridge TA, Heslop L, Gross JG, Oron U, Halevy O (2002) Low-energy laser irradiation promotes the survival and cell cycle entry of skeletal muscle satellite cells. J Cell Sci 115:1461–1469PubMed
35.
Zurück zum Zitat Oron U (2006) Photoengineering of tissue repair in skeletal and cardiac muscles. Photomed Laser Surg 24:111–120PubMedCrossRef Oron U (2006) Photoengineering of tissue repair in skeletal and cardiac muscles. Photomed Laser Surg 24:111–120PubMedCrossRef
36.
Zurück zum Zitat Iyomasa DM, Garavelo I, Iyomasa MM, Watanabe IS, Issa JP (2009) Ultrastructural analysis of the low-level laser therapy effects on the lesioned anterior tibial muscle in the gerbil. Micron 40:413–418PubMedCrossRef Iyomasa DM, Garavelo I, Iyomasa MM, Watanabe IS, Issa JP (2009) Ultrastructural analysis of the low-level laser therapy effects on the lesioned anterior tibial muscle in the gerbil. Micron 40:413–418PubMedCrossRef
37.
Zurück zum Zitat Dourado DM, Favero S, Matias R, Carvalho Pde T, da Cruz-Hofling MA (2011) Low-level laser therapy promotes vascular endothelial growth factor receptor-1 expression in endothelial and nonendothelial cells of mice gastrocnemius exposed to snake venom. Photochem Photobiol 87:418–426PubMedCrossRef Dourado DM, Favero S, Matias R, Carvalho Pde T, da Cruz-Hofling MA (2011) Low-level laser therapy promotes vascular endothelial growth factor receptor-1 expression in endothelial and nonendothelial cells of mice gastrocnemius exposed to snake venom. Photochem Photobiol 87:418–426PubMedCrossRef
Metadaten
Titel
GaAs 904-nm laser irradiation improves myofiber mass recovery during regeneration of skeletal muscle previously damaged by crotoxin
verfasst von
Lucila H. Silva
Meiricris T. Silva
Rita M. Gutierrez
Talita C. Conte
Cláudio A. Toledo
Marcelo S. Aoki
Richard E. Liebano
Elen H. Miyabara
Publikationsdatum
01.09.2012
Verlag
Springer-Verlag
Erschienen in
Lasers in Medical Science / Ausgabe 5/2012
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI
https://doi.org/10.1007/s10103-011-1031-x

Weitere Artikel der Ausgabe 5/2012

Lasers in Medical Science 5/2012 Zur Ausgabe