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Lasers in Medical Science

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01.02.2013 | Original Article

Biostimulatory effect of low-level laser therapy on keratinocytes in vitro

verfasst von: Fernanda G. Basso, Camila F. Oliveira, Cristina Kurachi, Josimeri Hebling, Carlos A. de Souza Costa

Erschienen in: Lasers in Medical Science | Ausgabe 2/2013

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Abstract

Epithelial cells play an important role in reparative events. Therefore, therapies that can stimulate the proliferation and metabolism of these cells could accelerate the healing process. To evaluate the effects of low-level laser therapy (LLLT), human keratinocytes were irradiated with an InGaAsP diode laser prototype (LASERTable; 780 ± 3 nm; 40 mW) using 0.5, 1.5, 3, 5, and 7 J/cm² energy doses. Irradiations were done every 24 h totaling three applications. Evaluation of cell metabolism (MTT assay) showed that LLLT with all energy doses promoted an increase of cell metabolism, being more effective for 0.5, 1.5, and 3 J/cm². The highest cell counts (Trypan blue assay) were observed with 0.5, 3, and 5 J/cm². No statistically significant difference for total protein (TP) production was observed and cell morphology analysis by scanning electron microscopy revealed that LLLT did not promote morphological alterations on the keratinocytes. Real-time polymerase chain reaction (qPCR) revealed that LLLT also promoted an increase of type I collagen (Col-I) and vascular endothelial growth factor (VEGF) gene expression, especially for 1.5 J/cm², but no change on fibroblast growth factor-2 (FGF-2) expression was observed. LLLT at energy doses ranging from 0.5 to 3 J/cm² promoted the most significant biostimulatory effects on cultured keratinocytes.

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Titel: Biostimulatory effect of low-level laser therapy on keratinocytes in vitro
verfasst von: Fernanda G. Basso
Camila F. Oliveira
Cristina Kurachi
Josimeri Hebling
Carlos A. de Souza Costa
Publikationsdatum: 01.02.2013
Verlag: Springer-Verlag
Erschienen in: Lasers in Medical Science / Ausgabe 2/2013
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-012-1057-8

Live-Webinar: Aktuelle Leitlinien bei Herz-Kreislauf-Erkrankungen

Springer Medizin

Biostimulatory effect of low-level laser therapy on keratinocytes in vitro

Abstract

Live-Webinar: Aktuelle Leitlinien bei Herz-Kreislauf-Erkrankungen

Springer Medizin

Abstract

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