nach oben

Erschienen in:

01.07.2013 | Original Article

DNA repair gene expression in biological tissues exposed to low-intensity infrared laser

verfasst von: Adenilson de Souza da Fonseca, Andre Luiz Mencalha, Vera Maria Araújo de Campos, Samara Cristina Ferreira Machado, Antonio Augusto de Freitas Peregrino, Mauro Geller, Flavia de Paoli

Erschienen in: Lasers in Medical Science | Ausgabe 4/2013

Einloggen, um Zugang zu erhalten

Abstract

Special properties of laser light have led to its usefulness in many applications in therapy. Excitation of endogenous chromophores in biotissues and generation of free radicals could be involved in its biological effects. DNA lesions induced by free radicals are repaired by base excision repair pathway. In this work, we evaluated the expression of APE1 and OGG1 genes related to repair of DNA lesions induced by free radicals. Skin and muscle tissues of Wistar rats were exposed to low-intensity infrared laser at different fluences and frequencies. After laser exposition of 1 and 24 h, tissue samples were withdrawn for total RNA extraction, cDNA synthesis, and evaluation of APE1 and OGG1 gene expression by quantitative polymerase chain reaction. Data obtained show that laser radiation alters the expression of APE1 and OGG1 mRNA differently in skin and muscle tissues of Wistar rats depending of the fluence, frequency, and time after exposure. Our study suggests that low-intensity infrared laser affects expression of genes involved in repair of DNA lesions by base excision repair pathway.

Karu T (1999) Primary and secondary mechanisms of action of visible to near-IR radiation on cells. J Photochem Photobiol B 49:1–17PubMedCrossRef

Niemz MH (2007) Laser–tissue interactions: fundamentals and applications. Springer, New York

Arisu HD, Türköz E, Bala O (2006) Effects of Nd:Yag laser irradiation on osteoblast cell cultures. Lasers Med Sci 21:175–180PubMedCrossRef

Chellini F, Sassoli C, Nosi D, Deledda C, Tonelli P, Zecchi-Orlandini S, Formigli L, Giannelli M (2010) Low pulse energy Nd:YAG laser irradiation exerts a biostimulative effect on different cells of the oral microenvironment: “an in vitro study”. Lasers Surg Med 42:527–539PubMedCrossRef

Chow RT, Johnson MI, Lopes-Martins RA, Bjordal JM (2009) Efficacy of low-level laser therapy in the management of neck pain: a systematic review and meta-analysis of randomised placebo or active-treatment controlled trials. Lancet 374(9705):1897–1908PubMedCrossRef

Lagan KM, Clements BA, McDonough S, Baxter GD (2001) Low intensity laser therapy (830 nm) in the management of minor post surgical wounds: a controlled clinical study. Lasers Surg Med 28:27–32PubMedCrossRef

Gao X, Xing D (2009) Molecular mechanisms of cell proliferation induced by low power laser irradiation. J Biomed Sci 16:4PubMedCrossRef

Peplow PV, Chung TY, Baxter GD (2010) Laser photobiomodulation of wound healing: a review of experimental studies in mouse and rat animal models. Photomed Laser Surg 28:291–325PubMedCrossRef

Eells JT, Wong-Riley MT, VerHoeve J, Henry M, Buchman EV, Kane MP, Gould LJ, Das R, Jett M, Hodgson BD, Margolis D, Whelan HT (2004) Mitochondrial signal transduction in accelerated wound and retinal healing by near-infrared light therapy. Mitochondrion 4:559–567PubMedCrossRef

10.

Karu TI, Pyatibrat LV, Afanasyeva NI (2005) Cellular effects of low power laser therapy can be mediated by nitric oxide. Lasers Surg Med 36:307–314PubMedCrossRef

11.

Hu W-P, Wang J-J, Yu C-L, Lan C-CE, Chen G-S, Yu H-S (2007) Helium–neon laser irradiation stimulates cell proliferation through photostimulatory effects in mitochondria. J Investigat Dermatol 127:2048–2057CrossRef

12.

Tafur J, Mills PJ (2008) Low-intensity light therapy: exploring the role of redox mechanisms. Photomed Laser Surg 26:321–326CrossRef

13.

Maiya GA, Kumar P, Rao L (2005) Effect of low intensity helium–neon (He–Ne) laser irradiation on diabetic wound healing dynamics. Photomed Laser Surg 23:187–190PubMedCrossRef

14.

Kushibiki T, Tajiri T, Ninomiya Y, Awazu K (2010) Chondrogenic mRNA expression in prechondrogenic cells after blue laser irradiation. J Photochem Photobiol B 98:211–215PubMedCrossRef

15.

Omasa S, Motoyoshi M, Arai Y, Ejima K, Shimizu N (2012) Low-level laser therapy enhances the stability of orthodontic mini-implants via bone formation related to BMP-2 expression in a rat model. Photomed Laser Surg 30:255–61PubMedCrossRef

16.

Fujimoto K, Kiyosaki T, Mitsui N, Mayahara K, Omasa S, Suzuki N, Shimizu N (2010) Low intensity laser irradiation stimulates mineralization via increased BMPs in MC3T3-E1 cells. Lasers Surg Med 42:519–526PubMedCrossRef

17.

Pereira LB, Chimello DT, Ferreira MR, Bachmann L, Rosa AL, Bombonato-Prado KF (2012) Low-level laser therapy influences mouse odontoblast-like cell response in vitro. Photomed Laser Surg 30:206–213PubMedCrossRef

18.

Basso FG, Oliveira CF, Kurachi C, Hebling J, Costa CA (2012) Biostimulatory effect of low-level laser therapy on keratinocytes in vitro. Lasers Med Sci in press

19.

Hamajima S, Hiratsuka K, Kiyama-Kishikawa M, Tagawa T, Kawahara M, Ohta M, Sasahara H, Abiko Y (2003) Effect of low-level laser irradiation on osteoglycin gene expression in osteoblasts. Lasers Med Sci 18:78–82PubMedCrossRef

20.

Yazdani SO, Golestaneh AF, Shafiee A, Hafizi M, Omrani HA, Soleimani M (2012) Effects of low level laser therapy on proliferation and neurotrophic factor gene expression of human Schwann cells in vitro. J Photochem Photobiol B 107:9–13PubMedCrossRef

21.

Zhang L, Zhao J, Kuboyama N, Abiko Y (2011) Low-level laser irradiation treatment reduces CCL2 expression in rat rheumatoid synovia via a chemokine signaling pathway. Lasers Med Sci 26:707–717PubMedCrossRef

22.

Mesquita-Ferrari RA, Martins MD, Silva JA Jr, da Silva TD, Piovesan RF, Pavesi VC, Bussadori SK, Fernandes KP (2011) Effects of low-level laser therapy on expression of TNF-α and TGF-β in skeletal muscle during the repair process. Lasers Med Sci 26:335–340PubMedCrossRef

23.

Joyce KM, Downes CS, Hannigan BM (1999) Radioadaptation in Indian muntjac fibroblast cells induced by low intensity laser irradiation. Mutat Res 435:35–42PubMedCrossRef

24.

Dube A, Bock C, Bauer E, Kohli R, Gupta PK (2001) He–Ne laser irradiation protects B-lymphoblasts from UVA-induced DNA damage. Radiat Environ Biophys 40:77–82PubMedCrossRef

25.

Zhang L, Hu Y (2002) Determination of laser-induced thymine–thymine dimer in DNA by LC. J Pharm Biomed Anal 29:95–102PubMedCrossRef

26.

Hawkins DH, Abrahamse H (2006) The role of laser fluence in cell viability, proliferation, and membrane integrity of wounded human skin fibroblasts following helium–neon laser irradiation. Lasers Surg Med 38:74–83PubMedCrossRef

27.

Kohli R, Gupta PK (2003) Irradiance dependence of He–Ne laser-induced protection against UVC radiation in E. coli strains. J Photochem Photobiol B: Biology 69:161–167PubMedCrossRef

28.

Houreld NN, Abrahamse H (2007) Effectiveness of helium–neon laser irradiation on viability and cytotoxicity of diabetic-wounded fibroblast cells. Photomed Laser Surg 25:474–481PubMedCrossRef

29.

Fonseca AS, Moreira TO, Paixão DL, Farias FM, Guimarães OR, Paoli S, Geller M, Paoli F (2010) Effect of laser therapy on DNA damage. Lasers Surg Med 42:481–488PubMedCrossRef

30.

Fonseca AS, Geller M, Valença SS, Paoli F (2012) Low level infrared laser effect on plasmid DNA. Lasers Med Sci 27:121–130PubMedCrossRef

31.

Fonseca AS, Presta GA, Geller M, Paoli F, Valença SS (2012) Low-intensity infrared laser increases plasma proteins and induces oxidative stress in vitro. Lasers Med Sci 27:211–217CrossRef

32.

Zhang Y, Song S, Fong C-C, Tsang C-H, Yang Z, Yang M (2003) cDNA microarray analysis of gene expression profiles in human fibroblast cells irradiated with red light. J Invest Dermatol 120:849–857PubMedCrossRef

33.

Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 25:402–408PubMedCrossRef

34.

Strauss PR, O'Regan NE (2001) Abasic site repair in higher eukaryotes. In: Nickoloff JA, Hoekstra MF (eds) DNA damage and repair: vol. 3: advances from phage to humans. Human Press, Totowa, pp 43–85CrossRef

35.

An N, Fleming AM, White HS, Burrows CJ (2012) Crown ether–electrolyte interactions permit nanopore detection of individual DNA abasic sites in single molecules. Proc Natl Acad Sci USA 109:11504–11509PubMedCrossRef

36.

Jacobs AL, Schär P (2012) DNA glycosylases: in DNA repair and beyond. Chromosoma 121:1–20PubMedCrossRef

37.

Klein HL, Hoot S (2011) Repair systems. In: Krebs JE, Goldstein ES, Kilpatrick ST (eds) Lewin's genes X. Jones and Bartlett, Sudbury

38.

Mitra S, Hazra TK, Roy R, Ikeda S, Biswas T, Lock J, Boldogh I, Izumi T (1997) Complexities of DNA base excision repair in mammalian cells. Mol Cells 7:305–312PubMed

39.

Ramana CV, Boldogh I, Izumi T, Mitra S (1998) Activation of apurinic/apyrimidinic endonuclease in human cells by reactive oxygen species and its correlation with their adaptive response to genotoxic of free radicals. Proc Natl Acad Sci USA 95:5061–5066PubMedCrossRef

40.

Bjoras M, Luna L, Johnsen B, Hoff E, Haug T, Rognes T, Seeberg E (1997) Opposite base-dependent reactions of a human base excision repair enzyme on DNA containing 7,8-dihydro-8-oxoguanine and abasic sites. EMBO J 16:6314–6322PubMedCrossRef

41.

Bigot K, Leemput J, Vacher M, Campalans A, Radicella JP, Lacassagne E, Provost A, Masson C, Menasche M, Abitbol M (2009) Expression of 8-oxoguanine DNA glycosylase (Ogg1) in mouse retina. Mol Vision 15:1139–1152

42.

Fonseca AS, Presta GA, Geller M, Paoli F (2011) Low intensity infrared laser induces filamentation in Escherichia coli cells. Laser Phys 21:1829–1837CrossRef

43.

Stadler I, Evans R, Kolb B, Naim JO, Narayan V, Buehner N, Lanzafame RJ (2000) In vitro effects of low-level laser irradiation at 660 nm on peripheral blood lymphocytes. Lasers Surg Med 27:255–261PubMedCrossRef

44.

Grossman N, Schneid N, Reuveni H, Halevy S, Lubart R (1998) 780 nm low power diode laser irradiation stimulates proliferation of keratinocyte cultures: involvement of reactive oxygen species. Lasers Surg Med 22:212–218PubMedCrossRef

45.

Tirlapur UK, Konig K, Peuckert C, Krieg R, Halbhuber K (2001) Femtosecond near-infrared laser pulses elicit generation of reactive oxygen species in mammalian cells leading to apoptosis-like death. Exp Cell Res 263:88–97PubMedCrossRef

46.

Fonseca AS, Teixeira AF, Presta GA, Geller M, Valença SS, Paoli F (2012) Low intensity infrared laser effects on Escherichia coli cultures and plasmid DNA. Laser Phys in press

47.

Avni D, Levkovitz S, Maltz L, Oron U (2005) Protection of skeletal muscles from ischemic injury: low-level laser therapy increases antioxidant activity. Photomed Laser Surg 23:273–277PubMedCrossRef

48.

Medrado AP, Soares AP, Santos ET, Reis SR, Andrade ZA (2008) Influence of laser photobiomodulation upon connective tissue remodeling during wound healing. J Photochem Photobiol B 92:144–152PubMedCrossRef

49.

Aimbire F, Albertini R, Pacheco MT, Castro-Faria-Neto HC, Leonardo PS, Iversen VV, Lopes-Martins RA, Bjordal JM (2006) Low-level laser therapy induces dose-dependent reduction of TNFalpha levels in acute inflammation. Photomed Laser Surg 24:33–37PubMedCrossRef

50.

Kim YG (2002) Laser mediated production of reactive oxygen and nitrogen species; implications for therapy. Free Rad Res 36:1243–1250CrossRef

51.

Karu T, Pyatibrat L, Kalendo G (1994) Irradiation with HeNe laser can influence the cytotoxic response of HeLa cells to ionizing radiation. Int J Radiat Biol 65:691–697PubMedCrossRef

52.

Andrade AC, Chrysis D, Audi L, Nilsson O (2011) Methods to study cartilage and bone development. Endocr Dev 21:52–66PubMedCrossRef

53.

Wesolowski R, Ramaswamy B (2011) Gene expression profiling: changing face of breast cancer classification and management. Gene Expr 15:105–115PubMedCrossRef

54.

Wu FR, Ding B, Qi B, Shang MB, Yang XX, Liu Y, Li WY (2012) Sequence analysis, expression patterns and transcriptional regulation of mouse Ifrg15 during preimplantation embryonic development. Gene in press

Titel: DNA repair gene expression in biological tissues exposed to low-intensity infrared laser
verfasst von: Adenilson de Souza da Fonseca
Andre Luiz Mencalha
Vera Maria Araújo de Campos
Samara Cristina Ferreira Machado
Antonio Augusto de Freitas Peregrino
Mauro Geller
Flavia de Paoli
Publikationsdatum: 01.07.2013
Verlag: Springer London
Erschienen in: Lasers in Medical Science / Ausgabe 4/2013
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-012-1191-3

Springer Medizin

Abstract

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 4/2013

Incremental treatments with laser therapy augments good behavioral outcome in the rabbit small clot embolic stroke model

Fox–Fordyce-like disease following laser hair removal appearing on all treated areas

Nanoleakage in primary teeth prepared by laser irradiation or bur

Low-level laser therapy improves crescentic glomerulonephritis in rats

Helium–neon laser improves bone repair in rabbits: comparison at two anatomic sites

Spatially controlled photothermal heating of bladder tissue through single-walled carbon nanohorns delivered with a fiberoptic microneedle device