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01.01.2015 | Original Article

Effect of pre-irradiation with different doses, wavelengths, and application intervals of low-level laser therapy on cytochrome c oxidase activity in intact skeletal muscle of rats

verfasst von: Gianna Móes Albuquerque-Pontes, Rodolfo de Paula Vieira, Shaiane Silva Tomazoni, Cláudia Oliveira Caires, Victoria Nemeth, Adriane Aver Vanin, Larissa Aline Santos, Henrique Dantas Pinto, Rodrigo Labat Marcos, Jan Magnus Bjordal, Paulo de Tarso Camillo de Carvalho, Ernesto Cesar Pinto Leal-Junior

Erschienen in: Lasers in Medical Science | Ausgabe 1/2015

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Abstract

Modulation of cytochrome c oxidase activity has been pointed as a possible key mechanism for low-level laser therapy (LLLT) in unhealthy biological tissues. But recent studies by our research group with LLLT in healthy muscles before exercise found delayed skeletal muscle fatigue development and improved biochemical status in muscle tissue. Therefore, the aim of this study was to evaluate effects of different LLLT doses and wavelengths in cytochrome c oxidase activity in intact skeletal muscle. In this animal experiment, we irradiated the tibialis anterior muscle of rats with three different LLLT doses (1, 3, and 10 J) and wavelengths (660, 830, and 905 nm) with 50 mW power output. After irradiation, the analyses of cytochrome c oxidase expression by immunohistochemistry were analyzed at 5, 10, 30 min and at 1, 2, 12, and 24 h. Our results show that LLLT increased (p < 0.05) cytochrome c oxidase expression mainly with the following wavelengths and doses: 660 nm with 1 J, 830 nm with 3 J, and 905 nm with 1 J at all time points. We conclude that LLLT can increase cytochrome c oxidase activity in intact skeletal muscle and that it contributes to our understanding of how LLLT can enhance performance and protect skeletal muscles against fatigue development and tissue damage. Our findings also lead us to think that the combined use of different wavelengths at the same time can enhance LLLT effects in skeletal muscle performance and other conditions, and it can represent a therapeutic advantage in clinical settings.

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Titel: Effect of pre-irradiation with different doses, wavelengths, and application intervals of low-level laser therapy on cytochrome c oxidase activity in intact skeletal muscle of rats
verfasst von: Gianna Móes Albuquerque-Pontes
Rodolfo de Paula Vieira
Shaiane Silva Tomazoni
Cláudia Oliveira Caires
Victoria Nemeth
Adriane Aver Vanin
Larissa Aline Santos
Henrique Dantas Pinto
Rodrigo Labat Marcos
Jan Magnus Bjordal
Paulo de Tarso Camillo de Carvalho
Ernesto Cesar Pinto Leal-Junior
Publikationsdatum: 01.01.2015
Verlag: Springer London
Erschienen in: Lasers in Medical Science / Ausgabe 1/2015
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-014-1616-2

Springer Medizin

Abstract

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