Skip to main content
Erschienen in: Lasers in Medical Science 3/2015

01.04.2015 | Original Article

Effect of low-level laser therapy on bone repair: a randomized controlled experimental study

verfasst von: Valéria Regina Gonzalez Sella, Fernando Russo Costa do Bomfim, Paula Carolina Dias Machado, Maria José Misael da Silva Morsoleto, Milton Chohfi, Helio Plapler

Erschienen in: Lasers in Medical Science | Ausgabe 3/2015

Einloggen, um Zugang zu erhalten

Abstract

The aim of this study was to investigate the effect of low-level laser therapy (LLLT) on bone repair in femoral fractures. Sixty adult Wistar rats were randomly assigned into one of two groups: group A (ostectomy + LLLT) or group B (ostectomy + sham laser). An experimental model of complete bone fracture was surgically created by removing a 2-mm fragment from the middle third of the femoral shaft. Data were analyzed on days 8, 13, and 18 after the fracture (subgroups 1, 2, and 3). Samples were assessed for changes in inflammatory infiltration; trabecular bone matrix, periosteal, and new bone formations; and changes in the expression of particular osteogenic-related proteins (osteocalcin, osteopontin, and osteonectin). Microscopic analysis revealed a significant decrease in inflammatory infiltration, intense trabecular bone matrix and periosteal formation, and an increase in newly formed bone after laser irradiation. We also found an increase in the expression of bone matrix proteins with LLLT, with a significant difference measured for osteocalcin in the LLLT group at day 8 (p = 0.007). We show that LLLT plays an important role in augmenting bone tissue formation, which is relevant to fracture healing. LLLT may therefore be indicated as an adjunct therapeutic tool in clinical practice for the treatment or recovery of nonunion injuries.
Literatur
1.
Zurück zum Zitat Oliveira AM, Castro-Silva II, Fernandes GVO, Melo BR, Alves ATNN, Júnior AS, Lima ICB, Granjeiro JM (2014) Effectiveness and acceleration os bone repair in critical-sized rat calvarial defects using low-level laser therapy. Laser Surg Med 46(1):61–67CrossRef Oliveira AM, Castro-Silva II, Fernandes GVO, Melo BR, Alves ATNN, Júnior AS, Lima ICB, Granjeiro JM (2014) Effectiveness and acceleration os bone repair in critical-sized rat calvarial defects using low-level laser therapy. Laser Surg Med 46(1):61–67CrossRef
2.
Zurück zum Zitat Carvalho P, Silva I, Reis F, Belchior A, Facco G, Guimarães R, Fernandes G, Denadai A (2006) Effect of 650 nm low-power laser on bone morphogenetic protein in bone defects induced in rat femors. Acta Cir Bras 21(suplem 4):63–68CrossRef Carvalho P, Silva I, Reis F, Belchior A, Facco G, Guimarães R, Fernandes G, Denadai A (2006) Effect of 650 nm low-power laser on bone morphogenetic protein in bone defects induced in rat femors. Acta Cir Bras 21(suplem 4):63–68CrossRef
3.
Zurück zum Zitat Claes L, Willie B (2007) The enhancement of bone regeneration by ultrasound. Prog Biophys Mol Biol 93(1–3):384–398CrossRefPubMed Claes L, Willie B (2007) The enhancement of bone regeneration by ultrasound. Prog Biophys Mol Biol 93(1–3):384–398CrossRefPubMed
4.
Zurück zum Zitat Haal BK (2005) Bones and cartilage: developmental and evolutionary skeletal biology. Academic, San Diego, CA Haal BK (2005) Bones and cartilage: developmental and evolutionary skeletal biology. Academic, San Diego, CA
6.
Zurück zum Zitat Childs S (2003) Stimulators of bone healing. Biologic and biomechanical. Orthop Nurs 22(6):421–428CrossRefPubMed Childs S (2003) Stimulators of bone healing. Biologic and biomechanical. Orthop Nurs 22(6):421–428CrossRefPubMed
7.
Zurück zum Zitat Giannunzio GA, Speerli RC, Guglielmotti MB (2008) Electrical field effect on peri-implant osteogenesis: a histologic and histomorphometric study. Implant Dent 17:118–126CrossRefPubMed Giannunzio GA, Speerli RC, Guglielmotti MB (2008) Electrical field effect on peri-implant osteogenesis: a histologic and histomorphometric study. Implant Dent 17:118–126CrossRefPubMed
9.
Zurück zum Zitat Hawkins-Evans D, Abrahamse H (2008) Efficacy of three different laser wavelengths for in vitro wound healing. Photodermatol Photoimmunol Photomed 24:199–210CrossRef Hawkins-Evans D, Abrahamse H (2008) Efficacy of three different laser wavelengths for in vitro wound healing. Photodermatol Photoimmunol Photomed 24:199–210CrossRef
10.
Zurück zum Zitat Marchesano L (2005) Comportamento de marcadores séricos de formação e reabsorção óssea após enxerto autógeno em fissure alveolar congênita: sem e com plasma rico em plaquetas. Doctoral, Universidade Estadual Paulista “Júlio de Mesquita Filho”, Araraquara Marchesano L (2005) Comportamento de marcadores séricos de formação e reabsorção óssea após enxerto autógeno em fissure alveolar congênita: sem e com plasma rico em plaquetas. Doctoral, Universidade Estadual Paulista “Júlio de Mesquita Filho”, Araraquara
11.
Zurück zum Zitat Santa Cruz Biotechnology I (2010) osteocalcin (FL-100): sc-30044 Santa Cruz Biotechnology I (2010) osteocalcin (FL-100): sc-30044
12.
Zurück zum Zitat Santa Cruz Biotechnology I (2010) Osteopontin (LFMb-14): sc-73631 Santa Cruz Biotechnology I (2010) Osteopontin (LFMb-14): sc-73631
13.
Zurück zum Zitat Santa Cruz Biotechnology I (2010) SPARC (AON-5031): sc-73472 instructions guide Santa Cruz Biotechnology I (2010) SPARC (AON-5031): sc-73472 instructions guide
14.
15.
Zurück zum Zitat Matkowskyj KA, Schonfeld D, Benya RV (2000) Quantitative immunohistochemistry by measuring cumulative signal strength using commercially available software photoshop and matlab. J Histochem Cytochem 48(2):303–311CrossRefPubMed Matkowskyj KA, Schonfeld D, Benya RV (2000) Quantitative immunohistochemistry by measuring cumulative signal strength using commercially available software photoshop and matlab. J Histochem Cytochem 48(2):303–311CrossRefPubMed
16.
Zurück zum Zitat Pham N, Morrison A, Schwock J, Aviel-Ronen S, Iakovlev V, Tsao M, Ho J, DW Hedley (2007) Quantitative image analysis of immunohistochemical stains using a CMYK color model. Diagn Pathol:2–8 Pham N, Morrison A, Schwock J, Aviel-Ronen S, Iakovlev V, Tsao M, Ho J, DW Hedley (2007) Quantitative image analysis of immunohistochemical stains using a CMYK color model. Diagn Pathol:2–8
18.
Zurück zum Zitat Drosse I, Volkmer E, Seitz S, Seitz H, Penzkofer R, Zahn K, Matis U, Mutschler W, Augat P, Schieker M (2008) Validation of a femoral critical size defect model for orthotopic evaluation of bone healing: a biomechanical, veterinary and trauma surgical perspective. Tissue Eng Part C Methods 14(1):79–88. doi:10.1089/tec.2007.0234 CrossRefPubMed Drosse I, Volkmer E, Seitz S, Seitz H, Penzkofer R, Zahn K, Matis U, Mutschler W, Augat P, Schieker M (2008) Validation of a femoral critical size defect model for orthotopic evaluation of bone healing: a biomechanical, veterinary and trauma surgical perspective. Tissue Eng Part C Methods 14(1):79–88. doi:10.​1089/​tec.​2007.​0234 CrossRefPubMed
20.
Zurück zum Zitat Silva Júnior A, Pinheiro A, Weismann MOR, Ramalho L, Nicolau R (2002) Computerized morphometric assessment os the effect os low level laser therapy on bone repair: an experimental study. J Clin Laser Med Surg 20(2):83–87CrossRefPubMed Silva Júnior A, Pinheiro A, Weismann MOR, Ramalho L, Nicolau R (2002) Computerized morphometric assessment os the effect os low level laser therapy on bone repair: an experimental study. J Clin Laser Med Surg 20(2):83–87CrossRefPubMed
21.
Zurück zum Zitat Abramoff MMF, Pereira MD, Alves MTS, Segreto RA, Guilherme A, Ferreira LM (2014) Low-level laser therapy on bone repair of rat tibiae exposed to ionizing radiation. Photomed Laser Surg 32(11):618–626. doi:10.1089/pho.2013.3692 CrossRef Abramoff MMF, Pereira MD, Alves MTS, Segreto RA, Guilherme A, Ferreira LM (2014) Low-level laser therapy on bone repair of rat tibiae exposed to ionizing radiation. Photomed Laser Surg 32(11):618–626. doi:10.​1089/​pho.​2013.​3692 CrossRef
23.
Zurück zum Zitat Garavello-Freitas I, Baranauskas V, Joazeiro P, Padovani C, Pai-Silva MD, Cruz-Hofling M (2003) Low-power laser irradiation improves histomorphometrical parameters and bone matrix organization during tibia wound healing in rats. J Photochem Photobiol B Biol 70(2):81–89CrossRef Garavello-Freitas I, Baranauskas V, Joazeiro P, Padovani C, Pai-Silva MD, Cruz-Hofling M (2003) Low-power laser irradiation improves histomorphometrical parameters and bone matrix organization during tibia wound healing in rats. J Photochem Photobiol B Biol 70(2):81–89CrossRef
24.
Zurück zum Zitat Freitas I, Baranauskas V, Cruz-Hofling M (2000) Laser effects on osteogenesis. Appl Surf Sci 154–155:548–554CrossRef Freitas I, Baranauskas V, Cruz-Hofling M (2000) Laser effects on osteogenesis. Appl Surf Sci 154–155:548–554CrossRef
25.
Zurück zum Zitat Favaro-Pipi E, Ribeiro DA, Ribeiro JU, Bossini P, Oliveira P, Parizotto NA, Tim C, de Araujo HS, Renno AC (2011) Low-level laser therapy induces differential expression of osteogenic genes during bone repair in rats. Photomed Laser Surg 29(5):311–317. doi:10.1089/pho.2010.2841 CrossRefPubMed Favaro-Pipi E, Ribeiro DA, Ribeiro JU, Bossini P, Oliveira P, Parizotto NA, Tim C, de Araujo HS, Renno AC (2011) Low-level laser therapy induces differential expression of osteogenic genes during bone repair in rats. Photomed Laser Surg 29(5):311–317. doi:10.​1089/​pho.​2010.​2841 CrossRefPubMed
26.
Zurück zum Zitat Barushka O, Yaakobi T, Oron U (1995) Effect of low-energy laser (He-Ne) irradiation on the process of bone repair in the rat tibia. Bone 16(1):47–55PubMed Barushka O, Yaakobi T, Oron U (1995) Effect of low-energy laser (He-Ne) irradiation on the process of bone repair in the rat tibia. Bone 16(1):47–55PubMed
27.
Zurück zum Zitat Friesen LR, Cobb CM, Rapley JW, Forgas-Brockman L, Spencer P (1999) Laser irradiation of bone: II. Healing response following treatment by CO2 and Nd:YAG lasers. J Periodont 70:75–83CrossRefPubMed Friesen LR, Cobb CM, Rapley JW, Forgas-Brockman L, Spencer P (1999) Laser irradiation of bone: II. Healing response following treatment by CO2 and Nd:YAG lasers. J Periodont 70:75–83CrossRefPubMed
28.
Zurück zum Zitat Guimarães KB (2006) Fotoengenharia do processo de reparo ósseo induzido pela laserterapia de baixa potência (GaAlAs): estudo em fêmures de ratos. Faculdade de Odontologia, PUCRS, Porto Alegre Guimarães KB (2006) Fotoengenharia do processo de reparo ósseo induzido pela laserterapia de baixa potência (GaAlAs): estudo em fêmures de ratos. Faculdade de Odontologia, PUCRS, Porto Alegre
29.
Zurück zum Zitat Giordano V, Knackfuss IG, Gomes RC, Giordano M, Mendonça RG, Coutynho F (2001) Influência do Laser de baixa energia no processo de consolidação da fratura de tibia: estudo experimental em ratos (Influency of low-level laser on healing process of tibial fracture: experimental study in rats). Rev Bras Ortop 36(5):174–178 Giordano V, Knackfuss IG, Gomes RC, Giordano M, Mendonça RG, Coutynho F (2001) Influência do Laser de baixa energia no processo de consolidação da fratura de tibia: estudo experimental em ratos (Influency of low-level laser on healing process of tibial fracture: experimental study in rats). Rev Bras Ortop 36(5):174–178
30.
Zurück zum Zitat Noda M, Denhardt D (2008) Osteopontin. In: AP I (ed) Principles of bone biology, 3rd edn, pp 351–366 Noda M, Denhardt D (2008) Osteopontin. In: AP I (ed) Principles of bone biology, 3rd edn, pp 351–366
35.
Zurück zum Zitat Tajali SB, MacDermid JC, Houghton P, Grewal R (2010) Effects of low power laser irradiation on bone healing in animals: a meta-analysis. J Orthop Surg Res 5:1. doi:10.1186/1749-799X-5-1 CrossRef Tajali SB, MacDermid JC, Houghton P, Grewal R (2010) Effects of low power laser irradiation on bone healing in animals: a meta-analysis. J Orthop Surg Res 5:1. doi:10.​1186/​1749-799X-5-1 CrossRef
36.
Zurück zum Zitat Park J, Kang K (2012) Effect of 980-nm GaAlAs diode laser irradiation on healing of extraction sockets in streptozotocin-induced diabetic rats: a pilot study. Lasers Med Sci 27:223–230CrossRefPubMed Park J, Kang K (2012) Effect of 980-nm GaAlAs diode laser irradiation on healing of extraction sockets in streptozotocin-induced diabetic rats: a pilot study. Lasers Med Sci 27:223–230CrossRefPubMed
Metadaten
Titel
Effect of low-level laser therapy on bone repair: a randomized controlled experimental study
verfasst von
Valéria Regina Gonzalez Sella
Fernando Russo Costa do Bomfim
Paula Carolina Dias Machado
Maria José Misael da Silva Morsoleto
Milton Chohfi
Helio Plapler
Publikationsdatum
01.04.2015
Verlag
Springer London
Erschienen in
Lasers in Medical Science / Ausgabe 3/2015
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI
https://doi.org/10.1007/s10103-015-1710-0

Weitere Artikel der Ausgabe 3/2015

Lasers in Medical Science 3/2015 Zur Ausgabe