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01.04.2015 | Original Article

The action of pre-exercise low-level laser therapy (LLLT) on the expression of IL-6 and TNF-α proteins and on the functional fitness of elderly rats subjected to aerobic training

verfasst von: Eliane Martins Amadio, Andrey Jorge Serra, Simone A. Guaraldo, José Antônio Silva Jr, Ednei Luis Antônio, Flávio Silva, Leslie Andrews Portes, Paulo José Ferreira Tucci, Ernesto Cesar Pinto Leal-Junior, Paulo de Tarso Camillo de Carvalho

Erschienen in: Lasers in Medical Science | Ausgabe 3/2015

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Abstract

The aim of the present study was to determine whether low-level laser therapy (LLLT), when used in conjunction with aerobic training, interferes with the expression of inflammatory markers IL-6 and TNF-α, thereby influencing the performance of old rats participating in swimming. A total of 30 Wistar rats (Rattus norvegicus albinus) were used for this study: 24 aged rats, and 6 young rats. The older animals were randomly divided into four groups designated as follows: aged-control, aged-exercise, aged-LLLT, aged-LLLT/exercise group, and young-control animals. Aerobic capacity (VO₂max) was analyzed before and after training period. The aged-exercise and aged-LLLT/exercise groups were trained for 6 weeks. LLLT laser was applied before each training session with 808 nm and 4 J of energy to the indicated groups throughout training. The rats were euthanized, and muscle tissue and serum were collected for muscle cross-sectional area and IL-6 and TNF-α protein analysis. In VO2 showed statistical difference between young- and aged-control groups (used as baseline) (p < 0.05). The same difference can be observed in the young control group compared with all intervention groups (exercise, LLLT and LLLT + exercise). In comparison with the aged-control group, a difference was observed only for comparison with the exercise group (p < 0.05), and exercise associated with LLLT group (p < 0.001). Levels of IL-6 and TNF-α for the aged-exercise and the aged-LLLT/exercise groups were significantly decreased compared to the aged-control group (p < 0.05). Analysis of the transverse section of the gastrocnemius muscle showed a significant difference between the aged-exercise and aged-LLLT/exercise groups (p < 0.001). These results suggest that laser therapy in conjunction with aerobic training may provide a therapeutic approach for reducing the inflammatory markers (IL-6 and TNF-α), however, LLLT without exercise was not able to improve physical performance of aged rats.

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Titel: The action of pre-exercise low-level laser therapy (LLLT) on the expression of IL-6 and TNF-α proteins and on the functional fitness of elderly rats subjected to aerobic training
verfasst von: Eliane Martins Amadio
Andrey Jorge Serra
Simone A. Guaraldo
José Antônio Silva Jr
Ednei Luis Antônio
Flávio Silva
Leslie Andrews Portes
Paulo José Ferreira Tucci
Ernesto Cesar Pinto Leal-Junior
Paulo de Tarso Camillo de Carvalho
Publikationsdatum: 01.04.2015
Verlag: Springer London
Erschienen in: Lasers in Medical Science / Ausgabe 3/2015
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-015-1713-x

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The action of pre-exercise low-level laser therapy (LLLT) on the expression of IL-6 and TNF-α proteins and on the functional fitness of elderly rats subjected to aerobic training

Abstract

Live-Webinar: Aktuelle Leitlinien bei Herz-Kreislauf-Erkrankungen

Springer Medizin

Abstract

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