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01.12.2015 | Original Article

Photodynamic inactivation of a multispecies biofilm using Photodithazine^® and LED light after one and three successive applications

verfasst von: Cristiane Campos Costa Quishida, Ewerton Garcia de Oliveira Mima, Lívia Nordi Dovigo, Janaina Habib Jorge, Vanderlei Salvador Bagnato, Ana Cláudia Pavarina

Erschienen in: Lasers in Medical Science | Ausgabe 9/2015

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Abstract

In this investigation, the effectiveness of successive applications of antimicrobial photodynamic inactivation (API) mediated by Photodithazine^® (PDZ) and LED light was evaluated against a multispecies biofilm formed by Candida albicans, Candida glabrata, and Streptococcus mutans on denture base acrylic resin. Standard cell suspensions (bacteria and yeast) were inoculated on acrylic resin samples, and the biofilm was grown for 48 h (37 °C/75 rpm). API was performed by the administration of PDZ (175 and 200 mg/L) and exposure to 37.5 J/cm² of LED light (660 nm). Additional samples were treated with PDZ or LED light only. Untreated control samples were not submitted to light or PDZ. The conditions described were applied once or in three consecutive applications for all groups. Cell viability was determined by colony counts (CFU/mL), metabolic activity, total biomass, and confocal laser scanning microscopy (CLSM). Data were analyzed by a nonparametric two-way ANOVA and Tukey tests (α = 0.05). The results obtained demonstrated a significant effect (p < 0.05) of number of applications and treatment groups for CFU/mL, and S. mutans showed the highest susceptibility to API. The metabolic activity of the multispecies biofilm was significantly reduced (p < 0.05) after API for both numbers of applications, which were also significantly different (p < 0.05) between them. The total biomass of the biofilm was significantly different (p < 0.05) only between groups submitted to one and three API applications. CLSM showed a visual increase of dead cells after API. API-mediated PDZ was effective in reducing the cell viability of multispecies biofilm. Three consecutive applications of API were more effective for reducing the cell viability and the total biomass of multispecies biofilm.

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Titel: Photodynamic inactivation of a multispecies biofilm using Photodithazine® and LED light after one and three successive applications
verfasst von: Cristiane Campos Costa Quishida
Ewerton Garcia de Oliveira Mima
Lívia Nordi Dovigo
Janaina Habib Jorge
Vanderlei Salvador Bagnato
Ana Cláudia Pavarina
Publikationsdatum: 01.12.2015
Verlag: Springer London
Erschienen in: Lasers in Medical Science / Ausgabe 9/2015
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-015-1811-9

Springer Medizin

Abstract

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