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16.03.2016 | Original Article

Low-level laser therapy modulates pro-inflammatory cytokines after partial tenotomy

verfasst von: Flávia Da Ré Guerra, Cristiano Pedrozo Vieira, Letícia Prado Oliveira, Petrus Pires Marques, Marcos dos Santos Almeida, Edson Rosa Pimentel

Erschienen in: Lasers in Medical Science | Ausgabe 4/2016

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Abstract

Tendon injuries give rise to substantial morbidity, and current understanding of the mechanisms involved in tendon injury and repair is limited. This lesion remains a clinical issue because the injury site becomes a region with a high incidence of recurrent rupture and has drawn the attention of researchers. We already demonstrated that low-level laser therapy (LLLT) stimulates the synthesis and organization of collagen I, MMP-9, and MMP-2 and improved the gait recovery of the treated animals. The aim of this study was to evaluate the effects of LLLT in the nitric oxide and cytokines profile during the inflammatory and remodeling phases. Adult male rats were divided into the following groups: G1—intact, G2— injured, G3—injured + LLLT (4 J/cm² continuous), G4—injured + LLLT (4 J/cm²–20 Hz—pulsed laser). According to the analysis, the animals were euthanized on different dates (1, 4, 8, or 15 days after injury). ELISA assay of TNF-α, IL-1β, IL-10, and TGF-β was performed. Western blotting of isoform of nitric oxide synthase (i-NOS) and nitric oxide dosage experiments was conducted. Our results showed that the pulsed LLLT seems to exert an anti-inflammatory effect over injured tendons, with reduction of the release of proinflammatory cytokines, such as TNF-α and the decrease in the i-NOS activity. Thanks to the pain reduction and the facilitation of movement, there was a stimulation in the TGF-β and IL-1β release. In conclusion, we believe that pulsed LLLT worked effectively as a therapy to reestablish the tendon integrity after rupture.

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Titel: Low-level laser therapy modulates pro-inflammatory cytokines after partial tenotomy
verfasst von: Flávia Da Ré Guerra
Cristiano Pedrozo Vieira
Letícia Prado Oliveira
Petrus Pires Marques
Marcos dos Santos Almeida
Edson Rosa Pimentel
Publikationsdatum: 16.03.2016
Verlag: Springer London
Erschienen in: Lasers in Medical Science / Ausgabe 4/2016
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-016-1918-7

Springer Medizin

Abstract

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