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30.07.2016 | Original Article

The effects of diode laser on Staphylococcus aureus biofilm and Escherichia coli lipopolysaccharide adherent to titanium oxide surface of dental implants. An in vitro study

verfasst von: Marco Giannelli, Giulia Landini, Fabrizio Materassi, Flaminia Chellini, Alberto Antonelli, Alessia Tani, Sandra Zecchi-Orlandini, Gian Maria Rossolini, Daniele Bani

Erschienen in: Lasers in Medical Science | Ausgabe 8/2016

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Abstract

Effective decontamination of biofilm and bacterial toxins from the surface of dental implants is a yet unresolved issue. This in vitro study aims at providing the experimental basis for possible use of diode laser (λ 808 nm) in the treatment of peri-implantitis. Staphylococcus aureus biofilm was grown for 48 h on titanium discs with porous surface corresponding to the bone-implant interface and then irradiated with a diode laser (λ 808 nm) in noncontact mode with airflow cooling for 1 min using a Ø 600-μm fiber. Setting parameters were 2 W (400 J/cm²) for continuous wave mode; 22 μJ, 20 kHz, 7 μs (88 J/cm²) for pulsed wave mode. Bactericidal effect was evaluated using fluorescence microscopy and counting the residual colony-forming units. Biofilm and titanium surface morphology were analyzed by scanning electron microscopy (SEM). In parallel experiments, the titanium discs were coated with Escherichia coli lipopolysaccharide (LPS), laser-irradiated and seeded with RAW 264.7 macrophages to quantify LPS-driven inflammatory cell activation by measuring the enhanced generation of nitric oxide (NO). Diode laser irradiation in both continuous and pulsed modes induced a statistically significant reduction of viable bacteria and nitrite levels. These results indicate that in addition to its bactericidal effect laser irradiation can also inhibit LPS-induced macrophage activation and thus blunt the inflammatory response. The λ 808-nm diode laser emerges as a valuable tool for decontamination/detoxification of the titanium implant surface and may be used in the treatment of peri-implantitis.

Zitzmann NU, Berglundh T (2008) Definition and prevalence of peri-implant diseases. J Clin Periodontol 35:286–291CrossRefPubMed

Lindhe J, Meyle J (2008) Group D of european workshop on periodontology. “Peri-implant diseases: consensus report of the sixth european workshop on periodontology”. J Clin Periodontol 35:282–285CrossRefPubMed

Nouneh RA, Wataha JC, Hanes PJ, Lockwood PE (2001) Effect of lipopolysaccharide contamination on the attachment of osteoblast-like cells to titanium and titanium alloy in vitro. J Oral Implantol 27:174–179CrossRefPubMed

Greenfield EM, Bi Y, Ragab AA, Goldberg VM, Nalepka JL, Seabold JM (2005) Does endotoxin contribute to aseptic loosening of orthopedic implants? J Biomed Mater Res B Appl Biomater 72:179–185CrossRefPubMed

Giannelli M, Pini A, Formigli L, Bani D (2011) Comparative in vitro study among the effects of different laser and LED irradiation protocols and conventional chlorhexidine treatment for deactivation of bacterial lipopolysaccharide adherent to titanium surface. Photomed Laser Surg 29:573–580CrossRefPubMed

Shrestha B, Theerathavaj ML, Thaweboon S, Thaweboon B (2012) In vitro antimicrobial effects of grape seed extract on peri-implantitis microflora in craniofacial implants. Asian Pac J Trop Biomed 2:822–825CrossRefPubMedPubMedCentral

Cortizo AM, Fernández Lorenzo de Mele MA (2012) Chlorhexidine delivery system from titanium/polybenzyl acrylate coating: evaluation of cytotoxicity and early bacterial adhesion. J Dent 40:329–337CrossRefPubMed

Zhao B, van der Mei HC, Subbiahdoss G, de Vries J, Rustema-Abbing M, Kuijer R, Busscher HJ, Ren Y (2014) Soft tissue integration versus early biofilm formation on different dental implant materials. Dent Mater 30:716–727CrossRefPubMed

Thurnheer T, Belibasakis GN (2015) Incorporation of staphylococci into titanium-grown biofilms: an in vitro "submucosal" biofilm model for peri-implantitis. Clin Oral Implants Res. doi:10.1111/clr.12715

10.

Harris LG, Richards RG (2004) Staphylococcus aureus adhesion to different treated titanium surfaces. J Mater Sci Mater Med 15:311–314CrossRefPubMed

11.

Nelson SK, Knoernschild KL, Robinson FG, Schuster GS (1997) Lipopolysaccharide affinity for titanium implant biomaterials. J Prosthet Dent 77:76–82CrossRefPubMed

12.

Dixon DR, Darveau RP (2005) Lipopolysaccharide heterogeneity: innate host responses to bacterial modification of lipid A structure. J Dent Res 84:584–595CrossRefPubMed

13.

Ozaki Y, Ukai T, Yamaguchi M et al (2009) Locally administered T cells from mice immunized with lipopolysaccharide (LPS) accelerate LPS-induced bone resorption. Bone 44:1169–1176CrossRefPubMed

14.

Renvert S, Samuelsson E, Lindahl C, Persson GR (2009) Mechanical non-surgical treatment of peri-implantitis: a double-blind randomized longitudinal clinical study. I: clinical results. J Clin Periodontol 36:604–609CrossRefPubMed

15.

Fox SC, Moriarty JD, Kusy RP (1990) The effects of scaling a titanium implant surface with metal and plastic instruments: an in vitro study. J Periodontol 61:485–490CrossRefPubMed

16.

Suarez F, Monje A, Galindo-Moreno P, Wang HL (2013) Implant surface detoxification: a comprehensive review. Implant Dent 22:465–473CrossRefPubMed

17.

Mettraux GR, Sculean A, Bürgin WB, Salvi GE (2015) Two-year clinical outcomes following non-surgical mechanical therapy of peri-implantitis with adjunctive diode laser application. Clin Oral Implants Res. doi:10.1111/clr.12689 PubMed

18.

Sahrmann P, Ronay V, Hofer D, Attin T, Jung RE, Schmidlin PR (2015) In vitro cleaning potential of three different implant debridement methods. Clin Oral Implants Res 26:314–319CrossRefPubMed

19.

Dörtbudak O, Haas R, Bernhart T, Mailath-Pokorny G (2001) Lethal photosensitization for decontamination of implant surfaces in the treatment of peri-implantitis. Clin Oral Implants Res 12:104–108CrossRefPubMed

20.

Javed F, Alghamdi AS, Ahmed A, Mikami T, Ahmed HB, Tenenbaum HC (2013) Clinical efficacy of antibiotics in the treatment of peri-implantitis. Int Dent J 63:169–176CrossRefPubMed

21.

Mouhyi J, Sennerby L, Pireaux JJ, Dourov N, Nammour S, Van Reck J (1998) An XPS and SEM evaluation of six chemical and physical techniques for cleaning of contaminated titanium implants. Clin Oral Implants Res 9:185–194CrossRefPubMed

22.

Mouhyi J, Sennerby L, Van Reck J (2000) The soft tissue response to contaminated and cleaned titanium surfaces using CO2 laser, citric acid and hydrogen peroxide. An experimental study in the rat abdominal wall. Clin Oral Implants Res 11:93–98CrossRefPubMed

23.

Kreisler M, Götz H, Duschner H (2002) Effect of Nd:YAG, Ho:YAG, Er:YAG, CO2, and GaAIAs laser irradiation on surface properties of endosseous dental implants. Int J Oral Maxillofac Implants 17:202–211PubMed

24.

Sculean A, Schwarz F, Becker J (2005) Anti-infective therapy with an Er:YAG laser: influence on peri-implant healing. Expert Rev Med Devices 2:267–276CrossRefPubMed

25.

Roncati M, Lucchese A, Carinci F (2013) Non-surgical treatment of peri-implantitis with the adjunctive use of an 810-nm diode laser. J Indian Soc Periodontol 17:812–815CrossRefPubMedPubMedCentral

26.

Aoki A, Mizutani K, Schwarz F, Sculean A, Yukna RA, Takasaki AA, Romanos GE, Taniguchi Y, Sasaki KM, Zeredo JL, Koshy G, Coluzzi DJ, White JM, Abiko Y, Ishikawa I, Izumi Y (2015) Periodontal and peri-implant wound healing following laser therapy. Periodontol 68:217–269CrossRef

27.

Giannini R, Vassalli M, Chellini F, Polidori L, Dei R, Giannelli M (2006) Neodymium:yttrium aluminum garnet laser irradiation with low pulse energy: a potential tool for the treatment of peri-implant disease. Clin Oral Implants Res 17:638–643CrossRefPubMed

28.

Romanos GE, Everts H, Nentwig GH (2000) Effects of diode and Nd: YAG laser irradiation on titanium discs: a scanning electron microscope examination. J Periodontol 71:810–815CrossRefPubMed

29.

Giannelli M, Bani D, Tani A, Pini A, Margheri M, Zecchi-Orlandini S, Tonelli P, Formigli L (2009) In vitro evaluation of the effects of low-intensity Nd:YAG laser irradiation on the inflammatory reaction elicited by bacterial lipopolysaccharide adherent to titanium dental implants. J Periodontol 80:977–984CrossRefPubMed

30.

Giannelli M, Lasagni M, Bani D (2015) Thermal effects of λ = 808 nm GaAlAs diode laser irradiation on different titanium surfaces. Lasers Med Sci 30:2341–2352CrossRefPubMed

31.

Romanos GE, Gutknecht N, Dieter S, Schwarz F, Crespi R, Sculean A (2009) Laser wavelengths and oral implantology. Lasers Med Sci 24:961–970CrossRefPubMed

32.

Subramani K, Wismeijer D (2012) Decontamination of titanium implant surface and re-osseointegration to treat peri-implantitis: a literature review. Int J Oral Maxillofac Implants 27:1043–1054PubMed

33.

Romanos GE, Gupta B, Yunker M, Romanos EB, Malmstrom H (2013) Lasers use in dental implantology. Implant Dent 22:282–288CrossRefPubMed

34.

Yan M, Liu M, Wang M, Yin F, Xia H (2015) The effects of Er:YAG on the treatment of peri-implantitis: a meta-analysis of randomized controlled trials. Lasers Med Sci 30:1843–1853CrossRefPubMed

35.

Harrison JJ, Stremick CA, Turner RJ, Allan ND, Olson ME, Ceri H (2010) Microtiter susceptibility testing of microbes growing on peg lids: a miniaturized biofilm model for high-throughput screening. Nat Protoc 5:1236–1254CrossRefPubMed

36.

Moncada S, Palmer RM, Higgs EA (1991) Nitric oxide: physiology, pathophysiology, and pharmacology. Pharmacol Rev 43:109–142PubMed

37.

Masini E, Nistri S, Vannacci A, Bani Sacchi T, Novelli A, Bani D (2004) Relaxin inhibits the activation of human neutrophils: involvement of the nitric oxide pathway. Endocrinology 145:1106–1112CrossRefPubMed

38.

Izquierdo-Barba I, García-Martín JM, Álvarez R, Palmero A, Esteban J, Pérez-Jorge C, Arcos D, Vallet-Regí M (2015) Nanocolumnar coatings with selective behavior towards osteoblast and Staphylococcus aureus proliferation. Acta Biomater 15:20–28CrossRefPubMed

39.

Aguayo S, Donos N, Spratt D, Bozec L (2015) Nanoadhesion of staphylococcus aureus onto titanium implant surfaces. J Dent Res 94:1078–1084CrossRefPubMed

40.

Lee A, Wang HL (2010) Biofilm related to dental implants. Implant Dent 19:387–393CrossRefPubMed

41.

Barão VA, Mathew MT, Assunção WG, Yuan JC, Wimmer MA, Sukotjo C (2011) The role of lipopolysaccharide on the electrochemical behavior of titanium. J Dent Res 90:613–618CrossRefPubMed

42.

Kreisler M, Kohnen W, Marinello C, Schoof J, Langnau E, Jansen B, d’Hoedt B (2003) Antimicrobial efficacy of semiconductor laser irradiation on implant surfaces. Int J Oral Maxillofac Implants 18:706–711PubMed

43.

Sennhenn-Kirchner S, Klaue S, Wolff N, Mergeryan H, Borg von Zepelin M, Jacobs HG (2007) Decontamination of rough titanium surfaces with diode lasers: microbiological findings on in vivo grown biofilms. Clin Oral Implants Res 18:126–132CrossRefPubMed

44.

Schoop U, Kluger W, Moritz A, Nedjelik N, Georgopoulos A, Sperr W (2004) Bactericidal effect of different laser systems in the deep layers of dentin. Lasers Surg Med 35:111–116CrossRefPubMed

45.

Mombelli A (2002) Microbiology and antimicrobial therapy of peri-implantitis. Periodontol 28:177–189CrossRef

Titel: The effects of diode laser on Staphylococcus aureus biofilm and Escherichia coli lipopolysaccharide adherent to titanium oxide surface of dental implants. An in vitro study
verfasst von: Marco Giannelli
Giulia Landini
Fabrizio Materassi
Flaminia Chellini
Alberto Antonelli
Alessia Tani
Sandra Zecchi-Orlandini
Gian Maria Rossolini
Daniele Bani
Publikationsdatum: 30.07.2016
Verlag: Springer London
Erschienen in: Lasers in Medical Science / Ausgabe 8/2016
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-016-2025-5

Springer Medizin

Abstract

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