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Erschienen in: Lasers in Medical Science 3/2019

22.09.2018 | Original Article

Biochemical changes in injured sciatic nerve of rats after low-level laser therapy (660 nm and 808 nm) evaluated by Raman spectroscopy

verfasst von: Melissa de Almeida Melo Maciel Mangueira, Nilton Maciel Mangueira, Ozimo Pereira Gama Filho, Márcio Moysés de Oliveira, Renato Albuquerque Heluy, Landulfo Silveira Jr, Egas Caparelli Moniz de Aragão Dáquer

Erschienen in: Lasers in Medical Science | Ausgabe 3/2019

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Abstract

The aim of this study was to identify biochemical changes in sciatic nerve (SN) after crush injury and low-level laser therapy (LLLT) with 660 nm and 808 nm by Raman spectroscopy (RS) analysis. A number of 32 Wistar rats were used, divided into four groups (control 1, control 2, LASER 660 nm, and LASER 808 nm). All animals underwent surgical procedure of the SN and groups control 2, LASER 660 nm, and LASER 808 nm were submitted to SN crush damage (axonotmesis). The LLLT in the groups LASER 660 nm and LASER 808 nm was applied daily for 21 consecutive days (100 mW, 30 s, 133 J/cm2 fluence). The hind paw was removed and the SN was dissected and positioned on an aluminum support to collect dispersive Raman spectra (830 nm excitation, 30 s accumulation). To estimate the biochemical changes in the SN associated with LLLT, the principal component analysis (PCA) was applied. The Raman spectra of the sciatic nerve fragments showed peaks of the major biochemical components of the nerve, especially sphingolipids, phospholipids, glycoproteins, and collagen. The spectral features identified in some of the principal component loading vectors are referred to the biochemical elements present on the SN and were increased in the groups treated with LLLT, mainly lipids (sphingo and phospholipids) and proteins (collagen)—constituents of the myelin sheath. The RS was effective in identifying the biochemical differences in the SN after the crush injury, and LASER 660 nm was more efficient than the LASER 808 nm in cell proliferation and repair of the injured SN.
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Metadaten
Titel
Biochemical changes in injured sciatic nerve of rats after low-level laser therapy (660 nm and 808 nm) evaluated by Raman spectroscopy
verfasst von
Melissa de Almeida Melo Maciel Mangueira
Nilton Maciel Mangueira
Ozimo Pereira Gama Filho
Márcio Moysés de Oliveira
Renato Albuquerque Heluy
Landulfo Silveira Jr
Egas Caparelli Moniz de Aragão Dáquer
Publikationsdatum
22.09.2018
Verlag
Springer London
Erschienen in
Lasers in Medical Science / Ausgabe 3/2019
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI
https://doi.org/10.1007/s10103-018-2627-1

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