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Erschienen in: Lasers in Medical Science 3/2020

31.08.2019 | Original Article

Photobiomodulation by dual-wavelength low-power laser effects on infected pressure ulcers

verfasst von: Andrezza Maria Côrtes Thomé Lima, Luiz Philippe da Silva Sergio, Larissa Alexsandra da Silva Neto Trajano, Bianca Paschoal de Souza, João Pedro da Motta Mendes, Adriano Fonseca Ribeiro Cardoso, Caroline Pisa Figueira, Beatriz dos Anjos Tavares, Daniella Sousa Figueira, André Luiz Mencalha, Eduardo Tavares Lima Trajano, Adenilson de Souza da Fonseca

Erschienen in: Lasers in Medical Science | Ausgabe 3/2020

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Abstract

The aim of this study was to evaluate the effects of photobiomodulation (PBM) by dual-wavelength low-power lasers on the healing and bacterial bioburden of pressure ulcer (PU) models. Twenty-five male Swiss mice were divided into five equal groups. Ischemia reperfusion cycles were employed to cause PU formation by the external application of magnetic plates. Immediately after wounding, a suspension of Pantoea agglomerans was applied at the base of all the wounds of the infected groups, using a calibrated pipette. PBM (simultaneous emission at 660 and 808 nm, 142.8 J/cm2, in continuous wave emission mode) was applied to the PUs for 14 sessions. The animals were euthanized 14 days after PU induction, and their tissues were analyzed for wound contraction and reepithelialization, epidermis thickness, bacterial survival, and IL-1β and IL-10 mRNA level evaluations. The PU areas appeared larger in the mice from the infected groups than in those in the laser group 4 days after PU induction and presented incomplete reepithelialization 14 days after PU induction. However, the PBM accelerated the wound healing in the infected + laser group compared with the infected group 11 and 14 days following the PU induction. The infected and irradiated PUs exhibited a thinner neo-epidermis than those in the infected group, and the bacterial survival decreased in the laser group; the relative expression IL-1β mRNA levels demonstrated an increasing tendency while the relative expression IL-10 mRNA levels demonstrated a decreasing tendency in the infected + laser and laser groups. These results suggest that PBM improves healing by killing or inhibiting bacteria in PUs as well as by accelerating the wound healing, resulting in tissue repair.
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Metadaten
Titel
Photobiomodulation by dual-wavelength low-power laser effects on infected pressure ulcers
verfasst von
Andrezza Maria Côrtes Thomé Lima
Luiz Philippe da Silva Sergio
Larissa Alexsandra da Silva Neto Trajano
Bianca Paschoal de Souza
João Pedro da Motta Mendes
Adriano Fonseca Ribeiro Cardoso
Caroline Pisa Figueira
Beatriz dos Anjos Tavares
Daniella Sousa Figueira
André Luiz Mencalha
Eduardo Tavares Lima Trajano
Adenilson de Souza da Fonseca
Publikationsdatum
31.08.2019
Verlag
Springer London
Erschienen in
Lasers in Medical Science / Ausgabe 3/2020
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI
https://doi.org/10.1007/s10103-019-02862-w

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