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Erschienen in: International Journal of Clinical Oncology 11/2019

15.07.2019 | Original Article

PLEKHG5 is a novel prognostic biomarker in glioma patients

verfasst von: Mingyu Qian, Zihang Chen, Shaobo Wang, Xiaofan Guo, Zongpu Zhang, Wei Qiu, Xiao Gao, Jianye Xu, Rongrong Zhao, Hao Xue, Gang Li

Erschienen in: International Journal of Clinical Oncology | Ausgabe 11/2019

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Abstract

Background

PLEKHG5, a Rho-specific guanine-nucleotide exchange factor, is involved in tumor cell migration, invasion and angiogenic potential. In this study, the expression pattern, prognostic value and function of PLEKHG5 in gliomas were investigated.

Methods

Immunohistochemistry was used to determine the expression pattern of PLEKHG5 in 61 glioma patients after curative resection. Statistical analysis was performed to evaluate the diagnostic and prognostic significance of PLEKHG5. Gene ontology (GO) analysis, Kyoto encyclopedia of genes and genomes (KEGG) pathway analysis and Gene set enrichment analysis (GSEA) were used to predict potential functions of PLEKHG5. Migration assay and western blot analysis determined PLEKHG5 function in glioma migration and invasion.

Results

Increased PLEKHG5 expression levels were associated with higher glioma grades (P < 0.05). In addition, glioblastomas multiforme have higher ratio and stronger intensity of PLEKHG5 expression compared with low-grade gliomas. High expression level of PLEKHG5 indicated poorer prognosis and shorter survival time in all glioma patients (P < 0.001). GO analysis, KEGG pathway analysis and GSEA analysis suggested that PLEKHG5 was involved in glioma migration, invasion and epithelial–mesenchymal transition. Migration assay and western blot analysis revealed PLEKHG5 promoted glioma migration and invasion.

Conclusion

Our results demonstrated PLEKHG5 could be used as a novel prognostic biomarker and anti-tumor target for glioma patients.
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Metadaten
Titel
PLEKHG5 is a novel prognostic biomarker in glioma patients
verfasst von
Mingyu Qian
Zihang Chen
Shaobo Wang
Xiaofan Guo
Zongpu Zhang
Wei Qiu
Xiao Gao
Jianye Xu
Rongrong Zhao
Hao Xue
Gang Li
Publikationsdatum
15.07.2019
Verlag
Springer Singapore
Erschienen in
International Journal of Clinical Oncology / Ausgabe 11/2019
Print ISSN: 1341-9625
Elektronische ISSN: 1437-7772
DOI
https://doi.org/10.1007/s10147-019-01503-0

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