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Erschienen in: Odontology 2/2011

01.07.2011 | Original Article

Dental caries experience in relation to salivary findings and molecular identification of S. mutans and S. sobrinus in subjects with Down syndrome

verfasst von: Aline Rogéria Freire de Castilho, Vanessa Pardi, Cássio Vicente Pereira

Erschienen in: Odontology | Ausgabe 2/2011

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Abstract

This study investigated the association between clinical and salivary or molecular parameters in Down syndrome subjects. Sixty individuals (1- to 48-year old) were clinically examined using DMFT/DMFS. Stimulated saliva was collected; salivary flow was calculated (mL/min), buffering capacity was measured using a standard pH tape. In addition, 25 μL of saliva was diluted using 10-fold-dilution method and then placed on Mitis-Salivarius-Bacitracin agar to count colony forming units (CFU/mL) of mutans streptococci. Polymerase chain reaction analysis identified species. Caries indexes were 0.65–13.5 (DMFT) and 0.65–26.0 (DMFS) according to groups. Ninety-four percent of subjects had low flow rate (0.7–1.0 mL/min) and 44% had low buffering capacity (pH < 4). Besides, 60% had more than 1 × 106 CFU/mL, 60% had S. mutans, and 41.4% had S. sobrinus. Caries indexes did not significantly correlate with flow rate, buffering capacity, CFU/mL by Pearson’s correlation (p > 0.05), and showed no significant association with prevalence of species by Chi-square (p > 0.05). There is no association between clinical picture and salivary or molecular parameters in Down syndrome subjects.
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Metadaten
Titel
Dental caries experience in relation to salivary findings and molecular identification of S. mutans and S. sobrinus in subjects with Down syndrome
verfasst von
Aline Rogéria Freire de Castilho
Vanessa Pardi
Cássio Vicente Pereira
Publikationsdatum
01.07.2011
Verlag
Springer Japan
Erschienen in
Odontology / Ausgabe 2/2011
Print ISSN: 1618-1247
Elektronische ISSN: 1618-1255
DOI
https://doi.org/10.1007/s10266-011-0010-9

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