Skip to main content

Advertisement

Log in

Survey of mutations of a histidine kinase gene BcOS1 in dicarboximide-resistant field isolates of Botrytis cinerea

  • DISEASE CONTROL
  • Published:
Journal of General Plant Pathology Aims and scope Submit manuscript

Abstract

Previously, we cloned a putative osmosensing histidine kinase gene (BcOS1) and revealed that a single amino acid substitution, isoleucine to serine at codon 365, conferred dicarboximide resistance in field isolates of Botrytis cinerea. This point mutation (type I) occurred within the restriction enzyme TaqI site of the wild-type BcOS1 gene. Thus, a procedure was developed for detecting the type I mutation of the BcOS1 gene using a polymerase chain reaction (PCR) in combination with restriction fragment-length polymorphism (RFLP). Diagnosis by PCR-RFLP was conducted on the 105 isolates isolated from 26 fields in Japan. All dicarboximide-sensitive isolates (49 isolates) had the wild-type BcOS1 gene, and the 43 isolates with the type I mutation were resistant to dicarboximides without exception. These data indicate that dicarboximide-resistant isolates with type I mutation are widespread throughout Japan. However, other types of dicarboximide resistance were detected among isolates from Osaka; among the 24 resistant isolates from Osaka, 12 had the BcOS1 gene without the type I mutation. BcOS1 gene sequencing of these resistant isolates classified them into two groups, type II and type III. The type II isolates have three amino acid substitutions within BcOS1p (368Val to Phe, 369Gln to His, and 447Thr to Ser). The type III isolates have two amino acid substitutions within BcOS1p (369Gln to Pro and 373Asn to Ser). These amino acid changes are located on the amino acid repeat domain in BcOS1p. The three types of resistant isolates were all moderately resistant to dicarboximides without significant osmotic sensitivity, and their pathogenicity on cucumber leaves was also very similar to that of the wild-type isolate.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • LA Alex KA Borkovich MI Simon (1996) ArticleTitleHyphal development in Neurospora crassa: involvement of a two-component histidine kinase Proc Natl Acad Sci USA 93 3416–3421 Occurrence Handle10.1073/pnas.93.8.3416 Occurrence Handle1:CAS:528:DyaK28XisVWitrs%3D Occurrence Handle8622950

    Article  CAS  PubMed  Google Scholar 

  • R Beever (1983) ArticleTitleOsmotic sensitivity of fungal variants resistant to dicarboximide fungicides Trans Br Mycol Soc 80 327–331

    Google Scholar 

  • W Cui RE Beever SL Parkes PL Weeds MD Templeton (2002) ArticleTitleAn osmosensing histidine kinase mediates dicarboximide fungicide resistance in Botryotinia fuckeliana (Botrytis cinerea) Fungal Genet Biol 36 187–198 Occurrence Handle10.1016/S1087-1845(02)00009-9 Occurrence Handle1:CAS:528:DC%2BD38XlsFKhsrc%3D Occurrence Handle12135574

    Article  CAS  PubMed  Google Scholar 

  • W Cui RE Beever SL Parkes MD Templeton (2004) ArticleTitleEvolution of an osmosensing histidine kinase in field strains of Botryotinia fuckeliana (Botrytis cinerea) in response to dicarboximide fungicide usage Phytopathology 94 1129–1135 Occurrence Handle1:CAS:528:DC%2BD2cXovVCjurc%3D

    CAS  Google Scholar 

  • IB Dry KH Yuan DG Hutton (2004) ArticleTitleDicarboximide resistance in field isolates of Alternaria alternata is mediated by a mutation in a two-component histidine kinase gene Fungal Genet Biol 41 102–108 Occurrence Handle10.1016/j.fgb.2003.09.002 Occurrence Handle1:CAS:528:DC%2BD3sXptF2qt70%3D Occurrence Handle14643263

    Article  CAS  PubMed  Google Scholar 

  • W Edlich H Lyr (1995) Mechanism of action of dicarboximide fungicides H Lyr (Eds) Modern selective fungicides – properties, applications, mechanisms of action Gustav Fischer New York 119–131

    Google Scholar 

  • M Fujimura N Ochiai A Ichiishi R Usami K Horikoshi I Yamaguchi (2000a) ArticleTitleFungicide resistance and osmotic stress sensitivity in os mutants of Neurospora crassa Pestic Biochem Physiol 67 125–133 Occurrence Handle10.1006/pest.2000.2479 Occurrence Handle1:CAS:528:DC%2BD3cXkslenurY%3D

    Article  CAS  Google Scholar 

  • M Fujimura N Ochiai A Ichiishi R Usami K Horikoshi I Yamaguchi (2000b) ArticleTitleSensitivity to phenylpyrrole fungicides and abnormal glycerol accumulation in os and cut mutant strains of Neurospora crassa J Pestic Sci 25 31–36 Occurrence Handle1:CAS:528:DC%2BD3cXhvVWnur0%3D

    CAS  Google Scholar 

  • M Fujimura N Ochiai T Motoyama I Yamaguchi (2002) Mechanism of resistance to dicarboximides and phenylpyrroles JM Clark I Yamaguchi (Eds) Agrochemical resistance; extent, mechanism, and detection American Chemical Society Washington DC 200–214

    Google Scholar 

  • M Fujimura N Ochiai M Oshima T Motoyama A Ichiishi R Usami K Horikoshi I Yamaguchi (2003) ArticleTitlePutative homologs of SSK22 MAPKK kinase and PBS2 MAPK kinase of Saccharomyces cerevisiae encoded by os-4 and os-5 genes for osmotic sensitivity and fungicide resistance in Neurospora crassa Biosci Biotech Biochem 67 186–191 Occurrence Handle1:CAS:528:DC%2BD3sXhtVWqtbw%3D

    CAS  Google Scholar 

  • M Grindle W Temple (1982) ArticleTitleFungicide resistance of os mutants of Neurospora crassa Neurospora Newsl 29 16–17

    Google Scholar 

  • K Kojima Y Takano A Yoshimi C Tanaka T Kikuchi T Okuno (2004) ArticleTitleFungicide activity through activation of a fungal signalling pathway Mol Microbiol 53 1785–1796 Occurrence Handle10.1111/j.1365-2958.2004.04244.x Occurrence Handle1:CAS:528:DC%2BD2cXotFyqurc%3D Occurrence Handle15341655

    Article  CAS  PubMed  Google Scholar 

  • YJ Lee H Hamamoto R Nakaune O Nawata Y Makizumi K Akutsu T Hibi (2001) ArticleTitleDistribution of the consensus sequences of ABC transporter gene among several taxonomically distinct phytopathogenic fungi J Gen Plant Pathol 67 106–110 Occurrence Handle1:CAS:528:DC%2BD3MXmtVGru7o%3D

    CAS  Google Scholar 

  • P Leroux R Fritz M Gredt (1983) Cross resistance between 3,5-dichlorophenyl cyclic imide fungicides and various aromatic compounds H Lyr C Polter (Eds) Systematische fungizide und antifungale verbindungen. Abh. Akad. d. Wiss. DDR Akademie-Verlag Berlin 79–88

    Google Scholar 

  • P Leroux C Lanen R Fritz (1992) ArticleTitleSimilarities in the antifungal activities of fenpiclonil, iprodione and tolclofos-methyl against Botrytis cinerea and Fusarium nivale Pestic Sci 36 255–261 Occurrence Handle1:CAS:528:DyaK3sXisV2ktrg%3D

    CAS  Google Scholar 

  • P Leroux R Fritz D Debieu C Albertini C Lanen J Bach M Gredt F Chapeland (2002) ArticleTitleMechanisms of resistance to fungicides in field strains of Botrytis cinerea Pest Manag Sci 58 876–888 Occurrence Handle10.1002/ps.566 Occurrence Handle1:CAS:528:DC%2BD38XmvFGjs74%3D Occurrence Handle12233177

    Article  CAS  PubMed  Google Scholar 

  • T Maeda SM Wurgler-Murphy H Saito (1994) ArticleTitleA two-component system that regulates an osmosensing MAP kinase cascade in yeast Nature 369 242–245 Occurrence Handle10.1038/369242a0 Occurrence Handle1:CAS:528:DyaK2cXkslyjsrs%3D Occurrence Handle8183345

    Article  CAS  PubMed  Google Scholar 

  • TK Miller S Renault CP Selitrennikoff (2002) ArticleTitleMolecular dissection of alleles of the osmotic-1 locus of Neurospora crassa Fungal Genet Biol 35 147–155 Occurrence Handle10.1006/fgbi.2001.1306 Occurrence Handle1:CAS:528:DC%2BD38Xht1Gns7o%3D Occurrence Handle11848677

    Article  CAS  PubMed  Google Scholar 

  • N Ochiai M Fujimura T Motoyama A Ichiishi R Usami K Horikoshi I Yamaguchi (2001) ArticleTitleCharacterization of mutations in the two-component histidine kinase gene that confer fludioxonil resistance and osmotic sensitivity in the os-1 mutants of Neurospora crassa Pest Manag Sci 57 437–442 Occurrence Handle10.1002/ps.302 Occurrence Handle1:CAS:528:DC%2BD3MXjsVektbo%3D Occurrence Handle11374161

    Article  CAS  PubMed  Google Scholar 

  • AB Orth A Sfarra EJ Pell M Tien (1992) ArticleTitleAn investigation into the role of lipid peroxidation in the mode of action of aromatic hydrocarbon and dicarboximide fungicides Pestic Biochem Physiol 44 91–100 Occurrence Handle10.1016/0048-3575(92)90106-A Occurrence Handle1:CAS:528:DyaK3sXitlOl

    Article  CAS  Google Scholar 

  • AB Orth M Rzhetskaya EJ Pell M Tien (1995) ArticleTitleA serine (threonine) protein kinase confers fungicide resistance in the phytopathogenic fungus Ustilago maydis Appl Environ Microbiol 61 2341–2345 Occurrence Handle1:CAS:528:DyaK2MXlvFOgtbg%3D Occurrence Handle7793954

    CAS  PubMed  Google Scholar 

  • M Oshima M Fujimura S Banno C Hashimoto T Motoyama A Ichiishi I Yamaguchi (2002) ArticleTitleA point mutation in the two-component histidine kinase BcOS-1 gene confers dicarboximide resistance in field isolates of Botrytis cinerea Phytopathology 92 75–80 Occurrence Handle1:CAS:528:DC%2BD38Xlt1yqsA%3D%3D

    CAS  Google Scholar 

  • E Pommer G Lorenz (1995) Dicarboximide fungicides H Lyr (Eds) Modern selective fungicides – properties, applications, mechanisms of action Gustav Fischer New York 99–118

    Google Scholar 

  • MA Ramesh RD Laidlaw F Dürrenberger AB Orth JW Kronstad (2001) ArticleTitleThe cAMP signal transduction pathway mediates resistance to dicarboximide and aromatic hydrocarbon fungicides in Ustilago maydis Fungal Genet Biol 32 183–193 Occurrence Handle10.1006/fgbi.2001.1258 Occurrence Handle1:CAS:528:DC%2BD3MXjtlSlur4%3D Occurrence Handle11343404

    Article  CAS  PubMed  Google Scholar 

  • M Schumacher MCS Enderlin CP Selitrennikoff (1997) ArticleTitleThe osmotic-1 locus of Neurospora crassa encodes a putative histidine kinase similar to osmosensors of bacteria and yeast Curr Microbiol 34 340–347 Occurrence Handle10.1007/s002849900193 Occurrence Handle1:CAS:528:DyaK2sXjsFaksbY%3D Occurrence Handle9142740

    Article  CAS  PubMed  Google Scholar 

  • JA Sweigard MJ Orbach B Valent FG Chumley (1990) ArticleTitleA miniprep procedure for isolating genomic DNA from Magnaporthe grisea Fungal Genet Newslett 37 44

    Google Scholar 

  • T Vermeulen H Schoonbeek MA De Waard (2001) ArticleTitleThe ABC transporter BcatrB from Botrytis cinerea is a determinant of the activity of the phenylpyrrole fungicide fludioxonil Pest Manag Sci 57 393–402 Occurrence Handle10.1002/ps.309 Occurrence Handle1:CAS:528:DC%2BD3MXjsVektLY%3D Occurrence Handle11374155

    Article  CAS  PubMed  Google Scholar 

  • HJ Vogel (1964) ArticleTitleDistribution of lysine pathways among fungi: evolutionary implications Am Nat 98 435–446 Occurrence Handle10.1086/282338 Occurrence Handle1:CAS:528:DyaF2MXmvVChsg%3D%3D

    Article  CAS  Google Scholar 

  • A Yoshimi M Tsuda C Tanaka (2004) ArticleTitleCloning and characterization of the histidine kinase gene Dic1 from Cochliobolus heterostrophus that confers dicarboximide resistance and osmotic adaptation Mol Genet Genom 271 228–236 Occurrence Handle1:CAS:528:DC%2BD2cXitVGktrs%3D

    CAS  Google Scholar 

  • Y Zhang R Lamm C Pillonel S Lam JR Xu (2002) ArticleTitleOsmoregulation and fungicide resistance: the Neurospora crassa os-2 gene encodes a HOG1 mitogen-activated protein kinase homologue Appl Environ Microbiol 68 532–538 Occurrence Handle1:CAS:528:DC%2BD38XhtV2gurY%3D Occurrence Handle11823187

    CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Makoto Fujimura.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Oshima, M., Banno, S., Okada, K. et al. Survey of mutations of a histidine kinase gene BcOS1 in dicarboximide-resistant field isolates of Botrytis cinerea. J Gen Plant Pathol 72, 65–73 (2006). https://doi.org/10.1007/s10327-005-0247-7

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10327-005-0247-7

Key words

Navigation