Abstract
Brevipalpus-transmitted viruses (BTV) cause chlorotic, necrotic and/or ringspot lesions in leaves and stems of orchids, citrus, coffee and several other plant species. There are two different types of BTVs, the nuclear and the cytoplasmic, based on maturation locale in the cell and particle morphology. The orchid fleck virus (OFV) is a BTV that infects orchids. Its short rodlike particles are 32–40 nm in diameter, 100–150 nm in length. OFV is found in the nucleus and is associated with intranuclear electronlucent viroplasms. In 1999, transmission electron microscopy analysis revealed a distinct type of virus causing orchid fleck symptoms. The bacilliform particles, 70–80 nm in diameter and 110–120 nm in length, induced electron-dense viroplasm inclusions in infected cells and resembled the cytoplasmic type associated with BTV, such as the citrus leprosis virus C. Our objective in the present study was to verify whether the cytoplasmic type virus found in orchids could be amplified using primers for other cytoplasmic BTVs, such as CiLV-C and Solanum violaefolium ringspot virus (SvRSV). Additionally, we aimed to differentiate the two BTVs found in orchids: the nuclear and the cytoplasmic types of OFV using microscopy and molecular and serological tools. This virus was not amplified by the CiLV-C and SvRSV primers, and neither the molecular nor the serological tools available to the OFV diagnosis reacted with it, demonstrating that they are definitely different viruses.
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References
Ali S, Lawson RH, Ishii M (1974) A bacilliform virus in white-streaked Dendrobium phalaenopsis flowers. Amer Orchid Soc Bull 43:529–533
Bastianel M, Freitas-Astúa J, Kitajima EW, Machado MA (2006) The citrus leprosis pathosystem. Summa Phytopathol 32:211–220
Blanchfield AL, Mackenzie AM, Gibbs A, Kondo H, Tamada T, Wilson CR (2001) Identification of orchid fleck virus by reverse transcriptase-polymerase chain reaction and analysis of isolate relationships. J Phytopathol 149:713–718
Chagas CM, Kitajima EW, Rodrigues JCV (2003) Coffee ringspot virus vectored by Brevipalpus phoenicis (Acari: Tenuipalpidae) in coffee. Exp Appl Acarol 30:203–213
Chang MU, Arai K, Doi Y, Yora K (1976) Morphology and intracellular appearance of orchid fleck virus. Ann Phytopathol Soc Jpn 42:156–167
Chang MU, Chun HH, Baek DH, Chung JD (1991) Studies on the viruses in orchids in Korea. 2. Dendrobium mosaic virus, odontoglossum ringspot virus, orchid fleck virus and unidentified potyvirus (in Korean with English summary). Korean J Plant Pathol 7:118–129
Childers CC, French JV, Rodrigues JCV (2003) Brevipalpus californicus, B. obovatus, B. phoenicis and B. lewisi (Acari: Tenuipalpidae): a review of their biology, feeding injury and economic importance. Exp Appl Acarol 30:5–28
Doi Y, Arai K, Yora K (1969) Ultrastructure of bacilliform virus found in Euonymus japonicum with mosaic and Cymbidium with ringspot (Abstract in Japanese). Ann Phytopathol Soc Jpn 35:388
Düvel D, Peters KR (1971) Virusähnlicke Partikel in Dendrobium antennatum (in German). Gartenwelt 3:52–54
Elliot MS, Zettler FW, Zimmerman MT, Barnett OW Jr, LeGrande MD (1996) Problems with interpretation of serological assays in a virus survey of orchid species from Puerto Rico, Ecuador, and Florida. Plant Dis 80:1160–1164
Ferreira PTO, Locali-Fabris EC, Freitas-Astúa J, Antonioli-Luizon R, Gomes RT, Machado M, Kitajima EW (2007) Characterization of a bacilliform virus isolated from Solanum violaefolium transmitted by the tenuipalpidae mites Brevipalpus phoenicis and Brevipalpus obovatus (Acari: Tenuipalpidae) (in Portuguese with English summary). Summa Phytopathol 33:264–269
Freitas-Astúa J, Rezende JAM, Kitajima EW (1999) Incidence of orchid viruses in the state of São Paulo, Brazil. Fitopatol Bras 24:125–130
Freitas-Astúa J, Moreira L, Rivera C, Rodríguez CM, Kitajima EW (2002) First report of orchid fleck virus in Costa Rica. Plant Dis 86:1402
Gibbs A, Mackenzie A (1997) A primer pair for amplifying part of the genome of all potyvirids by RT-PCR. J Virol Methods 63:9–16
Gibbs A, Mackenzie A, Blanchfield A, Cross P, Wilson C, Kitajima EW, Nightingale M, Clements M (2000) Viruses of orchids in Australia: their identification, biology and control. Aust Orchid Rev 65:10–21
Kitajima EW, Blumenschein A, Costa AS (1974) Rodlike particles associated with ringspot symptoms in several orchid species in Brazil. J Phytopathol 81:280–286
Kitajima EW, Kondo H, Mackenzie A, Rezende JAM, Gioria R, Gibbs A, Tamada T (2001) Comparative cytopathology and immunocytochemistry of Japanese, Australian and Brazilian isolates of orchid fleck virus. J Gen Plant Pathol 67:231–237
Kitajima EW, Chagas CM, Rodrigues JCV (2002) Há dois tipos morfológicos distintos de vírus, transmitidos pelo ácaro Brevipalpus, causando a leprose dos citros (Abstract in Portuguese). Anais do Semin Intern Citros-Melhoramento 7:119
Kitajima EW, Chagas CM, Rodrigues JCV (2003) Brevipalpus-transmitted plant virus and virus-like diseases: cytopathology and some recent cases. Exp Appl Acarol 30:135–160
Kitajima EW, Moraes GJ, Rodrigues JCV, Freitas-Astúa J (2006) Plantas infetadas naturalmente por vírus transmitidos pelo ácaro Brevipalpus (Acari: Tenuipalpidae) (Abstract in Portuguese). Anais Simp Bras Acarologia (Viçosa) 1:251
Kondo H, Matsumoto J, Maeda T, Inouye N (1995) Host range and some properties of orchid fleck virus isolated from oriental Cymbidium in Japan (in Japanese with English summary). Bull Res Inst Bioresour Okayama Univ 3:151–161
Kondo H, Maeda T, Tamada T (2003) Orchid fleck virus: Brevipalpus californicus mite transmission, biological properties, and genome structure. Exp Appl Acarol 30:215–223
Kondo H, Maeda T, Shirako Y, Tamada T (2006) Orchid fleck virus is a rhabdovirus with an unusual bipartite genome. J Gen Virol 87:2413–2421
Kubo KS, Antonioli-Luizon R, Freitas-Astúa J, Locali EC, Stuart RM, Machado MA, Kitajima EW (2006a) Estudo da variabilidade genética do orchid fleck virus (OFV) por SSCP (Abstract in Portuguese). Summa Phytopathol 32:S29
Kubo KS, Antonioli-Luizon R, Freitas-Astúa J, Machado MA, Kitajima EW (2006b) Otimização da diagnose molecular da mancha anular da orquídea (Abstract in Portuguese). Summa Phytopathol 32(Suppl):S30
Lesemann D, Begtrup J (1971) Electron microscopic demonstration of bacilliform virus in Phalaenopsis (in German with English summary). J Phytopathol 71:257–269
Locali EC, Freitas-Astúa J, Souza AA, Takita MA, Astúa-Monge G, Antonioli R, Kitajima EW, Machado MA (2003) Development of a molecular tool for the diagnosis of leprosis, a major threat to citrus production in the Americas. Plant Dis 87:1317–1321
Locali-Fabris EC, Freitas-Astúa J, Souza AA, Takita MA, Astúa-Monge G, Antonioli-Luizon R, Rodrigues V, Targon MLDN, Machado MA (2006) Complete nucleotide sequence, genomic organization and phylogenetic analysis of Citrus leprosis virus cytoplasmic type. J Gen Virol 87:2721–2729
Locali-Fabris EC, Freitas-Astúa J, Machado MA (2008) Citrus leprosis virus C (CiLV-C): Membro-tipo de um novo gênero de vírus de plantas. Revis Anu Patol Plantas 16:185–206
Maeda T, Kondo H, Misuhata K, Tamada T (1998) Evidence that orchid fleck virus is efficiently transmitted in a persistent manner by the mite Brevipalpus californicus (Abstract). Proc Int Cong Plant Pathol 7:13–18
Mowat WP, Dawson S (1987) Detection and identification of plant viruses by ELISA using crude sap extracts and unfractionated antisera. J Virol Methods 15:233–247
Pascon RC, Kitajima JP, Breton MC, Assumpção L, Greggio C, Zanca AS, Okura VK, Alegria MC, Camargo ME, Silva GGC, Cardozo JC, Vallim MA, Franco SF, Silva VH, Jordão H Jr, Oliveira F, Giachetto PF, Ferrari F, Aguilar-Vildoso CI, Franchiscini FJB, Silva JMF, Arruda P, Ferro JA, Reinach F, da Silva ACR (2006) The complete nucleotide sequence and genomic organization of citrus leprosis associated virus, cytoplasmatic type (CiLV-C). Virus Genes 32:289–298
Petzold H (1971) Electron microscopic demonstration of bacilliform virus in Dendrobium (in German with English summary). J Phytopathol 70:43–52
Rodrigues JCV, Kitajima EW, Childers CC, Chagas CM (2003) Citrus leprosis virus vectored by Brevipalpus phoenicis (Acari: Tenuipalpidae) on citrus in Brazil. Exp Appl Acarol 30:161–179
Sambrook J, Fritsch EF, Maniatis T (eds) (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor
Valverde RA, Nameth ST, Jordan RL (1990) Analysis of double-stranded RNA for plant virus diagnosis. Plant Dis 74:255–258
Acknowledgments
This work was part of the MSc thesis (Plant Pathology, Universidade de São Paulo) of the senior author. The authors would like to express their gratitude to Dr. Hideki Kondo (Research Institute for Bioresources, Okayama University, Japan) for providing OFV antiserum to FAPESP for a scholarship to the first author and to FAPESP and EMBRAPA for partially funding this work.
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Kubo, K.S., Freitas-Astúa, J., Machado, M.A. et al. Orchid fleck symptoms may be caused naturally by two different viruses transmitted by Brevipalpus . J Gen Plant Pathol 75, 250–255 (2009). https://doi.org/10.1007/s10327-009-0167-z
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DOI: https://doi.org/10.1007/s10327-009-0167-z