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Magnetic Resonance Materials in Physics, Biology and Medicine

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01.02.2014 | Short Communication

Visualization of immune cell infiltration in experimental viral myocarditis by ¹⁹F MRI in vivo

verfasst von: Christoph Jacoby, Nadine Borg, Philipp Heusch, Martina Sauter, Florian Bönner, Reinhard Kandolf, Karin Klingel, Jürgen Schrader, Ulrich Flögel

Erschienen in: Magnetic Resonance Materials in Physics, Biology and Medicine | Ausgabe 1/2014

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Abstract

Objective

This paper introduces a new approach permitting for the first time a specific, non-invasive diagnosis of myocarditis by visualizing the infiltration of immune cells into the myocardium.

Materials and methods

The feasibility of this approach is shown in a murine model of viral myocarditis. Our study uses biochemically inert perfluorocarbons (PFCs) known to be taken up by circulating monocytes/macrophages after intravenous injection.

Results

In vivo ¹⁹F MRI at 9.4 T demonstrated that PFC-loaded immune cells infiltrate into inflamed myocardial areas. Because of the lack of any fluorine background in the body, detected ¹⁹F signals of PFCs are highly specific as confirmed ex vivo by flow cytometry and histology.

Conclusion

Since PFCs are a family of compounds previously used clinically as blood substitutes, the technique described in our paper holds the potential as a new imaging modality for the diagnosis of myocarditis in man.

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Titel: Visualization of immune cell infiltration in experimental viral myocarditis by 19F MRI in vivo
verfasst von: Christoph Jacoby
Nadine Borg
Philipp Heusch
Martina Sauter
Florian Bönner
Reinhard Kandolf
Karin Klingel
Jürgen Schrader
Ulrich Flögel
Publikationsdatum: 01.02.2014
Verlag: Springer Berlin Heidelberg
Erschienen in: Magnetic Resonance Materials in Physics, Biology and Medicine / Ausgabe 1/2014
Print ISSN: 0968-5243
Elektronische ISSN: 1352-8661
DOI: https://doi.org/10.1007/s10334-013-0391-6

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Visualization of immune cell infiltration in experimental viral myocarditis by ¹⁹F MRI in vivo

Abstract

Objective

Materials and methods

Results

Conclusion

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Abstract

Objective

Materials and methods

Results

Conclusion

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