Skip to main content

Advertisement

SpringerLink
Log in

Detection of Brevipalpus-transmitted viruses in their mite vectors by RT–PCR

  • Published:
Experimental and Applied Acarology Aims and scope Submit manuscript

Abstract

The diagnosis of plant diseases caused by Brevipalpus-transmitted viruses (BrTVs) has been done through the analyses of symptoms, transmission electron microscopy, and RT–PCR of infected plant tissues. Here, we report the detection of Citrus leprosis virus C, Orchid fleck virus, Clerodendrum chlorotic spot virus and Solanum violaefolium ringspot virus in their viruliferous vectors Brevipalpus spp. using specific primer pairs for each of the viruses. The efficiency of virus transmission by Brevipalpus mites is low, so the detection of these pathogens in their vectors could constitute an important tool for studies involving virus-vector relationships, transmission, and monitoring the pathogen prior to the appearance of symptoms in the field.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Bassanezi RB, Laranjeira FF (2007) Spatial patterns of leprosis and its mite vector in commercial citrus groves in Brazil. Plant Pathol 56:97–106

    Article  Google Scholar 

  • Bastianel M, Novelli VM, Kitajima EW, Kubo KS, Bassanezi RB, Machado MA, Freitas-Astúa J (2010) Citrus Leprosis: Centennial of an Unusual Mite Virus Pathosystem. Plant Dis 94:284–292

    Article  Google Scholar 

  • Blanchfield AL, Mackenzie AM, Gibbs A, Kondo H, Tamada T, Wilson CR (2001) Identification of Orchid fleck virus by reverse transcriptase-polymerase chain reaction and analysis of isolate relationships. J Phytopathol 149:713–718

    Article  CAS  Google Scholar 

  • Boaretto MAC, Chiavegato LG (1994) Transmissão da leprose por ácaros Brevipalpus phoenicis (Geijskes, 1939) (Acari: Tenuipalpidae) temporariamente mantido sem hospedeirosintermediários em condições de laboratório. Científica 22:81–83

    Google Scholar 

  • Boaretto MAC, Chiavegato LG, Silva CAD (1993) Transmissão da leprose através de fêmeas de Brevipalpus phoenicis (GEIJSKES, 1939) (Acari: Tenuipalpidae) e de seus descendentes, em condições de laboratório. Científica 21:245–253

    Google Scholar 

  • Boari AJ, Freitas-Astúa J, Ferreira PTO, Neder DG, Nogueira NL, Rossi ML, Kitajima EW (2004) Purification and serology of the Coffee ringspot virus. Summa Phytopathol 30:453–458

    CAS  Google Scholar 

  • Carstens EB (2010) Ratification vote on taxonomic proposals to the International Committee on Taxonomy of Viruses (2009). Arch Virol 155:133–146

    Article  PubMed  CAS  Google Scholar 

  • Ferreira PTO, Locali EC, Freitas-Astúa J, Antonioli-Luizon R, Gomes RT, Machado MA, Kitajima EW (2007) Caracterização de um vírus baciliforme isolado de Solanum violaefolium transmitido pelos ácaros Brevipalpus phoenicis e Brevipalpus obovatus (Acari: Tenuipalpidae). Summa Phytopathol 33:264–269

    Article  CAS  Google Scholar 

  • Gibbs A, Mackenzie A (1997) A primer pair for amplifying part of the genome of all potyvirids by RT-PCR. J Virol Methods 63:9–16

    Article  PubMed  CAS  Google Scholar 

  • Kitajima EW, Chagas CM, Rodrigues JCV (2003) Brevipalpus-transmitted plant virus and virus-like diseases: cytopathology and some recent cases. Exp Appl Acarol 30:135–160

    Article  PubMed  CAS  Google Scholar 

  • Kitajima EW, Kubo KS, Ferreira PTO, Alcantara BK, Boari AJ, Gomes RT, Freitas-Astúa J, Rezende JAM, Moraes GJ, Salaroli RB (2008) Clerodendron chlorotic spot: a disease caused by a nuclear type of Brevipalpus (Acari: Tenuipalpidae)-transmitted virus. Sci Agric 65:36–49

    Article  Google Scholar 

  • Kitajma EW, Rodrigues JCV, Freitas-Astúa J (2010) An annotated list of ornamentals naturally found infected by Brevipalpus mite transmitted viruses. Sci Agric 67:348–371

    Google Scholar 

  • Knorr LC (1968) Studies on the etiology of leprosis in citrus. Proc. IV Int. Conf. Org. Citrus Virol. 4:332–340. Riverside, CA

    Google Scholar 

  • Kondo K, Maeda T, Shirako Y, Tamada T (2006) Orchid fleck virus is a rhabdovirus with an unusual bipartite genome. J Gen Virol 87:2413–2421

    Article  PubMed  CAS  Google Scholar 

  • Kondo H, Maeda T, Tamada T (2003) Orchid fleck virus: Brevipalpus californicus mite transmission, biological properties and genome structure. Exp Appl Acarol 30:215–223

    Article  PubMed  Google Scholar 

  • Kubo KS, Stuart RM, Freitas-Astúa J, Antonioli-Luizon R, Locali-Fabris EC, Coletta-Filho HD, Machado MA, Kitajima EW (2009) Evaluation of the genetic variability of Orchid fleck virus by single-strand conformational polymorphism analysis and nucleotide sequencing of a fragment from the coat protein gene. Arch Virol 154:1009–1014

    Article  PubMed  CAS  Google Scholar 

  • Locali EC, Freitas-Astúa J, Souza AA, Takita MA, Astúa-Monge G, Antonioli R, Kitajima EW, Machado MA (2003) Development of a molecular tool for the diagnosis of leprosis, a major threat to citrus production in the Americas. Plant Dis 87:1317–1321

    Article  CAS  Google Scholar 

  • Novelli VM, Freitas-Astúa J, Bastianel M, Locali-Fabris EC, Antonioli-Luizon R, Ribeiro BM, Machado MA (2007) Detecção do vírus da leprose dos citros no ácaro vetor. Laranja 28:39–46

    Google Scholar 

Download references

Acknowledgements

To FAPESP and Embrapa for partially supporting this work.

Author information

Authors and Affiliations

  1. Centro de Citricultura Sylvio Moreira/IAC, Rod. Anhanguera Km 158, CP 4, Cordeirópolis, SP, 13490-970, Brazil

    K. S. Kubo, V. M. Novelli, M. Bastianel, E. C. Locali-Fabris, R. Antonioli-Luizon, M. A. Machado & J. Freitas-Astúa

  2. Institute of Biology, CP 6109, State University of Campinas, UNICAMP, Campinas, SP, 13083-970, Brazil

    K. S. Kubo

  3. Embrapa Cassava and Tropical Fruits, Rua Embrapa s/n, Cruz das Almas, BA, 44380-000, Brazil

    J. Freitas-Astúa

Authors
  1. K. S. Kubo
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. V. M. Novelli
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. Bastianel
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. E. C. Locali-Fabris
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. R. Antonioli-Luizon
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. M. A. Machado
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. J. Freitas-Astúa
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to J. Freitas-Astúa.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kubo, K.S., Novelli, V.M., Bastianel, M. et al. Detection of Brevipalpus-transmitted viruses in their mite vectors by RT–PCR. Exp Appl Acarol 54, 33–39 (2011). https://doi.org/10.1007/s10493-011-9425-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10493-011-9425-9

Keywords

Search

Navigation