Abstract
The multi host tick, Hyalomma anatolicum, is the commonest Hyalomma species in India and cattle serves as the main host of this species. A study to evaluate the acaricide resistance of H. anatolicum to deltamethrin, cypermethrin and diazinon was conducted in 20 areas located in three agro climatic regions known to have abundance of the species. Results obtained by the “larval packet test” (LPT) showed a low grade resistance (level-I, RF <5) in the tick species to both deltamethrin and cypermethrin in 10 areas and higher grade resistance (level-II, RF <25) to deltamethrin in one area, where intensive use of synthetic pyrethroids are practiced for tick control. Low grade resistance to diazinon (level I) was recorded in six areas where organophosphates compounds are extensively used for agricultural practices allowing increased exposure of the moulting instars of the ticks to these chemicals. Biochemical analysis of the samples suggested involvement of esterase and alterations of acetylcholinesterase in the resistance mechanisms.
Similar content being viewed by others
References
Alvarez V, Bonilla R, Chacon I (1999) Situacion de la resistancia de la garrapata Boophilus microplus (Canestrini, 1887) a organofosforados y piretriides en Costa Rica. Rev. Ciencias Vet 22:41–60
Baxter GD, Barker SC (1998) Acetylcholinesterase cDNA of the cattle tick, Boophilus microplus: characterization and role in organophosphate resistance. Insect Biochem Mol Biol 28:581–589
Baxter GD, Green P, Stutten M, Barker SC (1999) Detecting resistance to organophosphates and carbamates in a cattle tick Boophilus microplus, with a propoxur based biochemical test. Exp Appl Acarol 23:907–914
Bhattacharyulu Y, Chaudhri RP, Gill BS (1975) Transtadial transmission of Theileria annulata through common ixodid ticks infesting Indian cattle. Parasitology 71:1–7
Biswas S (2003) Effects of ticks on animal production system. In: proceedings the natural seminar Leather Industry in today’s perspective. 14–15 November, Kolkata, India
Bradford MM (1976) A rapid and sensitive method for quantification of microgram quantities of protein utilizing the principles of protein dye binding. Anal Biochem 72:248–254
Castro-Janer E, Rifran L, Piaggio J, Gil A, Miller RJ, Schumaker TTS (2009) In vitro tests to establish LC50 and discriminating concentrations for fipronil against Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) and their standardization. Vet Parasitol 162:120–128
Castro-Janer E, Martins JR, Mendes MC, Namindome A, Klafke GM, Schumaker TT (2010) Diagnoses of fipronil resistance in Brazilian cattle ticks [Rhipicephalus (Boophilus) microplus] using in vitro larval bioassays. Vet Parasitol 173:300–306
FAO (1971) Recommended methods for the detection and measurement of resistance of agricultural pests to pesticides. Tendative method for larvae of cattle ticks, Boophilus spp. FAO method No. 7. FAO Plant Prot Bull 19:15
FAO (1984) Acaricide resistance: Test kit Instructions for use. World Acaricide Resistance Reference Centre, WARCC, Berlin
Finney DJ (1971) Probit analysis, 3rd edn. Cambridge University Press, Cambridge, UK
Foil LD, Coleman- Eisler M, Fragoso-Sanches H, Garcia-Vanquez Z, Guerrero FD, Jonsson NN, Langstaff IC, Li AY, Machila N, Miller RJ, Pruett JH, Torr S (2004) Factors that influence the prevalence of acaricide resistance and tick borne diseases. Vet Parasitol 125:163–181
Furlong J (1999) diagnostico de la susceptibilidad de la garrapata del Ganado Boophilus microplus a los acaricides en el estado de Minas Gerais, Brasil. In: IV seminario internacional de parasitologia Animal, Puerto Vallarta, Jalisco, Mexico, 20–22 de octubre de, 41–46
Geeverghese G, Dhanda V (1987) The Indian hyalomma ticks (Ixodoidea: Ixodidae). ICAR, New Delhi, pp 31–59
Ghosh S, Azhahianambi P (2007) Laboratory rearing of Theileria annulata free Hyalomma anatolicum anatolicum ticks. Exp Appl Acarol 43:137–146
Gordon SW, Linthicum KJ, Moulton JR (1993) Transmission of crimean-congo haemorrhagic fever virus in two species of Hyalomma ticks from infected adults to co feeding immature forms. Am J Trop Med Hyg 48:576–580
Harley KLS (1966) Studies on the survival of the nonparasitic stages of the cattle tick Boophilus microplus in three climatically dissimilar districts of north Queensland. Aust J Agric Res 17:387–410
Hemingway J (1998) Techniques to detect insecticide resistance mechanism (field and laboratory manual). Document WHO/CTD/CPC/MAL/98.6. World Health Organization, Geneva
Hemingway J, Ranson H (2000) Insecticide resistance in insect vectors of human diseases. Annu Rev Entomol 45:369–389
Jamroz RC, Guerrero FD, Pruett JH, Oehler DD, Miller RJ (2000) Molecular and biochemical survey of acaricide resistance mechanisms in larvae from Mexican strains of the southern cattle tick, Boophilus microplus. J Insect Physiol 46:685–695
Jonsson NN, Miller RJ, Robertson JL (2007) Critical evaluation of the modified-adult immersion test with discriminating dose bioassay for Boophilus microplus using American and Australian isolates. Vet Parasitol 146:307–315
Karti SS, Odabasi Z, Korten V, Yilmaz M, Sonmez M, Caylan R, Akdogan E, Eren N, Koksal I, Ovali E, Erickson BR, Vincent MJ, Nichol ST, Comer JA, Rollin PE, Ksiazek KG (2004) Crimean-congo hemorrhagic fever in Turkey. Emerg Infect Dis 10:1379–1384
Kumar S, Paul S, Sharma AK, Kumar R, Tewari SS, Chaudhuri P, Ray DD, Rawat AKS, Ghosh S (2011) Diazinon resistant status in Rhipicephalus (Boophilus) microplus collected from different agro-climatic regions of India. Vet Parasitol 181:274–281
Luguru SM, Banda DS, Pegram RG (1984) Susceptibility of ticks to acaricides in Zambia. Trop Anim Health Prod 16:21–26
Mekonnen S, Bryson NR, Fourie LJ, Peter RJ, Spickett AM, Taylor RJ, Strydom T, Horak IG (2002) Acaricide resistance profiles of single-and multi-host ticks from communal and commercial farming areas in the Eastern Cape and North-West Provinces of South Africa. Onderstepoort J Vet Res 69:99–105
Miller TA (1988) Mechanisms of resistance to pyrethroid insecticides. Parasitol Today 4:S8–S12
Miller RJ, Davey RB, George JE (2007) First report of permethrin resistant Boophilus microplus (Acari: Ixodidae) collected within the USA. J Med Entomol 44:308–315
Mohammed YO (2003) Base-line data on susceptibility of some ixodid tick species to Lindane in the Sudan. The Sudan J Vet Res 18:93–98
Morales SG, Fragoso SH, Garcia VZ (1999) Proceedings IV seminario internacional de parasitologia animal; control de la resistencia en garrapatas y moscas deimportancia veterinaria y enfermedades que transmiten, 20–22 October. Puerto Vallarta, Jalisco,México, p 264
Nolan J (1985) Mechanisms of resistance to chemicals in arthopods parasites of veterinary importance. Vet Parasitol 18:155–166
Nolan J (1990) Acaricide resistance in single and multi-host ticks and strategies for control. Parasitologia 32(1):145–153
Rodriguez Vivas RI, Rivas AL, Chowell G, Fragoso SH, Rosario CR, Garcıǐa VZ, Smith SD, Williams JJ, Schwager SJ (2007) Spatial distribution of acaricide profiles (Boophilus microplus strains susceptible or resistant to acaricides) in south-eastern Mexico. Vet Parasitol 146:158–169
Rosario-Cruz R, Almazan C, Miller RJ, Dominguez-Gracia DI, Hernandez-Ortiz R, de la Fuente J (2009) Genetic basis and impact of tick acaricide resistance. Front Biosci 14:2657–2665
Sangwan AK, Chhabra MB, Singh S (1993) Acaricide resistance status of common livestock ticks of Haryana. Indian Vet J 70:20–24
Sen SK, Fletcher TB (1962) Veterinary entomology and acarology for India. Indian Council of Agricultural Research, New Delhi, p 668
Shah-Fisher M, Raph-Say R (1989) Manual of tropical veterinary parasitology. CAB International, Wallingford, UK
Sharma Anil Kumar, Rinesh Kumar, Sachin Kumar, Gaurav Nagar, Nirbhay Singh, Sumer Singh Rawat, M L Dhakad, Rawat AKS, Ray DD, Srikant Ghosh (2012) Deltamethrin and cypermethrin resistance status of Rhipicephalus (Boophilus) microplus collected from six agro-climatic regions of India. Vet Parasitol (in press)
Springell PH (1974) The cattle tick in relation to animal production in Australia. Wild Anim Rev (FAO) 10:19–23
Villarino MA, Waghela SD, Wagner GG (2003) Biochemical detection of esterases in the adult female integument of organopsosphate resistant Boophilus microplus (Acari: Ixodidae). J Med Entomol 40:52–57
Wharton RH, Roulston WJ (1970) Resistance to ticks to chemicals. Ann Rev Entomol 15:381–404
Acknowledgments
The authors are grateful to Indian Council of Agricultural Research, New Delhi for funding through World Bank funded National Agricultural Innovation Project No. NAIP/Comp-4/C2066/2008. Senior author is thankful to Indian Veterinary Research Institute for providing research fellowship.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Shyma, K.P., Kumar, S., Sharma, A.K. et al. Acaricide resistance status in Indian isolates of Hyalomma anatolicum . Exp Appl Acarol 58, 471–481 (2012). https://doi.org/10.1007/s10493-012-9592-3
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10493-012-9592-3