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Effect of hypoxia on immunological, physiological response, and hepatopancreatic metabolism of juvenile Chinese mitten crab Eriocheir sinensis

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Abstract

The juvenile Chinese mitten crabs Eriocheir sinensis (1.40 ± 0.43 g) were cultured in water of 5.34 ± 0.43 (hypoxia, treatment group) and 21.02 ± 0.06 kPa (high dissolved oxygen (DO), control group) DO for 24 h, respectively. The total hemocyte counts (THC), the hyaline hemocyte counts were measured at 0 and 24 h in the treatment group, and superoxide dismutase (SOD) activity, concentrations of lactic acid and hemocyanin (Hc) in hemolymph and the metabolism of hepatopancreas (concentrations of glucose, total cholesterol, uric acid, total protein, urea, triglyceride in hepatopancreas) were assayed at 0, 2, 8, and 24 h in both treatment and control groups. The hyaline hemocyte counts and THC decreased significantly by 66 and 49% after exposure to hypoxic water for 24 h, respectively. SOD activity, Hc and lactic acid content were significantly affected in treatment group, and there were significant differences between treatment and control groups. In the treatment group, the concentration of glucose, cholesterol and uric acid in hepatopancreas had a significant variation for 24 h. The concentration of total protein, urea and triglyceride between treatment and control groups was not significantly different after 0, 2, 8, 24 h. However, there were significant differences between treatment and control groups in terms of total protein, glucose, and uric acid concentration. Taken together, the effect of hypoxia is comprehensive in juvenile Chinese mitten crab Eriocheir sinensis.

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Abbreviations

DO:

Dissolved oxygen

THC:

Total hemocyte counts

SOD:

Superoxide dismutase

Hc:

Hemocyanin

TC:

Total cholesterol

TG:

Triglyceride

Glu:

Glucose

UA:

Uric acid

ROIs:

Reactive oxygen intermediates

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Acknowledgments

This work was supported by the innovation research group developing project in the universities of Shanghai (nutrition, feed and environment of animal aquaculture, the second batch) and National Natural Sciences Foundation of China (Grant: 30871927). The authors would like to thank Que Youqing, Shao Luchang, Shen Tong, Wei Chengkai and Ji Ruijing in my laboratory for their supports in sampling, and thank Mr. Shen Hong (the manager of Muyu crab farm) for the help of sample supply. The experiments comply with the current Chinese animal care and manipulation legislation.

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  1. Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University, No 999 Huchenghuan Road, Lingang New District, Shanghai, 201306, People’s Republic of China

    Renjie Qiu, Yongxu Cheng, Xuxiong Huang, Xugan Wu, Xiaozhen Yang & Rui Tong

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  1. Renjie Qiu
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  2. Yongxu Cheng
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  3. Xuxiong Huang
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Correspondence to Yongxu Cheng.

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Qiu, R., Cheng, Y., Huang, X. et al. Effect of hypoxia on immunological, physiological response, and hepatopancreatic metabolism of juvenile Chinese mitten crab Eriocheir sinensis . Aquacult Int 19, 283–299 (2011). https://doi.org/10.1007/s10499-010-9390-z

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